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Citations for Becton, Dickinson and Company :
2201 - 2250 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Synthetic Biology 2023Quote: ... The exconjugants were picked with Pickolo colonypicker and inoculated into 96 deep-well plates containing 1.5 mL of TSB medium (Tryptic soy broth, BD 211825, Thermo Fisher Scientific) supplemented with apramycin (50 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: Blood samples were collected from each participant using 8.5 mL purple-cap Vacutainer EDTA tubes (Becton, Dickinson and Company, NJ, USA, Catalog #367861). The tubes were gently inverted 8–10 times post-collection for anticoagulant mixing ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Cell Biology 2023Quote: ... aeruginosa PAO1 was diluted 100-fold into 300 mL of M9 medium (1% w/v 5X M9 Salts (BD Difco, Franklin Lakes, NJ), 1.5% w/v Bacto Casamino Acids with low iron and salt content (BD Difco) ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ cells were cultured in 24-well round-bottom plates (1×106 cells/well) with plate-bound anti-CD3e (2.5 μg/ml, clone 2C11, BD Biosciences, San Diego, CA), soluble anti-CD28 (2 µg/ml ...
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bioRxiv - Systems Biology 2019Quote: ... three aliquots of the previously generated pool of random Agrobacterium tumefaciens insertional mutants were thawed on ice then inoculated at OD (600 nm) 0.2 in 100 mL YPD (BD Biosciences, BD242820, San Jose, CA) in a 250 mL baffled flask and incubated 8 hours at 30 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Chromosomal DNA was then stained by 4’,6-diamidine-2’-phenylindole (DAPI) at final concentration 2 μg/ml and the chromosome suspensions were analysed by FACSAria SORP II flow sorter (BD Biosciences, San Jose, USA) at rates of 1000-2000 particles/sec ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... was sprayed on the plantar surface of the hind right paw using a syringe of 1 ml (Tuberculin slip tip, BD, Franklin Lake, NJ, USA). Paw nociceptive responses ...
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bioRxiv - Microbiology 2020Quote: ... or 0 to 10−2 fluorescent intensity (AF647-negative) were captured and segregated in 2 separate 5 ml round-bottom tubes (BD Biosciences, San Jose, CA) containing 1% BSA/PBS ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated as indicated and stained with fluorescent conjugates of annexin-V and PI (1 µg/ml final concentration) and analyzed on a FACSCanto machine (BD, Franklin Lakes, NJ, USA). Annexin V was prepared as previously described (Brumatti et al. ...
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bioRxiv - Biophysics 2022Quote: ... Cell suspensions were homogenized by passing 10 times through a precooled 1-ml syringe with a 27-gauge stainless steel needle (BD Biosciences, Franklin Lakes, NJ). The homogenate was adjusted to about 40% sucrose by the addition of 700μl of 60% sucrose prepared in TNE and placed at the bottom of an ultracentrifuge tube ...
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bioRxiv - Microbiology 2021Quote: ... near-coral seawater was sampled 2-5 cm away from each coral colony prior to tissue sampling via negative pressure with a 60 ml Luer-lock syringe (BD, Franklin Lakes, NJ, USA). Two seawater samples were collected over each colony displaying SCTLD lesions ...
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bioRxiv - Immunology 2021Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlugTM, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Physiology 2020Quote: A 10 mL blood sample was drawn from an antecubital vein in the forearm and collected into a serum vacutainer (BD Vacutainer systems, Plymouth, UK). The blood samples were obtained at each time point and left at 22-24°C for 30 min to allow clotting ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... IPF-HLFs or nHLFs were cultured in 24-well plates previously coated with 10 mg/mL growth factor-reduced (GFR) Matrigel (BD Biosciences, San Jose, California). Following 48 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Propidium iodide (PI) (1.4 μg/mL) was added five minutes before cells were analyzed using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA). Cells negative for both annexin-V and PI staining were considered viable.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) strain harboring pCOLD-1-EhPTEN1 was grown at 37°C in 50 ml of LB medium (BD Difco, New Jersey, USA) in the presence of 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... coli was suspended in 1 ml of spent supernatant to 0.05 OD600 and inoculated atop solid LB agar in a 6-well plate (BD Biosciences Inc., Franklin Lakes, NJ).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole blood was collected from human volunteer donors with informed consent in 8 to 10 BD Vacutainer CPT 8 ml tubes (BD Biosciences, San Jose, CA). The blood samples were mixed immediately prior to centrifugation by gently inverting tubes 8-10 times ...
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bioRxiv - Microbiology 2022Quote: ... a loopful from frozen glycerol stock will be aseptically transferred into 10 ml Tryptic Soy Broth plus 0.6% yeast extract (TSBYE) (Difco, Becton, Dickinson and Company, Sparks, Md.), (BeanTown Chemical) ...
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bioRxiv - Immunology 2023Quote: ... 100 µg/ml streptomycin, and 10% fetal bovine serum before stimulation with 1X stimulation cocktail (eBiosciences, Waltham MA) with Golgi Plug (BD Biosciences, Franklin Lakes, NJ) before flow cytometry for 4-6 hours ...
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bioRxiv - Immunology 2023Quote: ... a total of 50 ml of whole human blood was collected by a trained phlebotomist from healthy donors in BD Vacutainer® EDTA tubes (BD Vacutainer®, USA). This was previously approved by the RVC’s Ethics and Welfare Committee (URN 2019 1916-3).
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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bioRxiv - Cell Biology 2023Quote: ... Blood samples (10 ml) were collected every two hours from each of the four participants into lithium heparin Vacutainers (BD Biosciences, Franklin Lakes, NJ) and separated by centrifugation ...
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bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were counter-stained with DAPI at a final concentration of 1 ng/mL and analyzed on a BD LSR Fortessa instrument (BD Biosciences, Franklin Lakes, NJ). The resulting data was processed using the software FlowJo (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: Invasion assays were done in 8 μm pore size of the Chemotaxicell chamber coated with 100 μg/ml Growth factor reduced Matrigel (BD Biosciences, San Jose CA) for 3 h at 37°C and blocked with 0.1% bovine serum albumin in PBS ...
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bioRxiv - Genetics 2023Quote: ... containing penicillin/streptomycin (50U/ml) and 10% FBS and plated onto 12mm Poly-D-Lysine/Laminin pre-coated coverslips (BD Biosciences, Franklin Lakes, NJ). Cells were incubated overnight at 37°C and imaged the following day (sixteen to twenty-four hours after plating cells ...
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bioRxiv - Immunology 2024Quote: ... 100 µg/ml streptomycin, and 10% fetal bovine serum before stimulation with 1X stimulation cocktail (eBiosciences, Waltham MA) with Golgi Plug (BD Biosciences, Franklin Lakes, NJ) before flow cytometry for 4-6 hours ...
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bioRxiv - Microbiology 2023Quote: ... A 50 µL inoculum was given via the last left proleg with a 0.5 ml 29G Micro-Fine U-100 insulin injection unit (BD Medical, Franklin Lakes, NJ, USA).
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml TNF-α or exposed to hypoxia by placing the plate in a BD GasPak™ EZ Anaerobe Pouch System (BD Biosciences, 260683). After 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting single cells were then diluted to 106 cells/ml in PBS and directly analyzed for EGFP fluorescence on a FACSCalibur instrument using CellQuest software (BD Biosciences, San Jose, CA, USA). EGFP-positive cells were identified by comparison with single-cell suspensions obtained from the testes of wild-type chicks.
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bioRxiv - Neuroscience 2020Quote: ... Explants were grown on 12-mm diameter glass coverslips coated with 20 μg/mL laminin for 2D cultures used for time-lapse imaging or coated with 7 μl of Matrigel™ (BD Biosciences, Mississauga, ON, Canada) for 3D cultures used for morphological analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... treated with 50 ng/ml cytokines or mixed with MSCs in the upper part of Matrigel coated Boyden chambers (8 μM, BD Biosciences, San Jose, CA, USA). Cell invasion was determined by analysing the number of labelled cells that migrated through the Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µL of PI (from 50 µg/ml stock solution) was then added and the cell cycle was analyzed by flow cytometry (BD FACSCanto™ II, BD Bioscience) using untreated cells as negative control for gating ...
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bioRxiv - Cell Biology 2022Quote: ... 12 mL of blood was collected (one drawing) from each individual into two BD Vacutainer® K3-EDTA-coated collection tubes (BD Biosciences, San Jose, USA) (Figure 10 – step I) ...