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Citations for Becton, Dickinson and Company :
2101 - 2150 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Immunology 2024Quote: ... Stimulated and unstimulated cells were harvested and washed with 2 mL of FACS buffer and then cells were fixed with BD transcription factor fixation/perm buffer (cat #562574, BD Bioscience) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The suspension was filtered once more through a 70 μM cell strainer and was resuspended at ∼1x107 cells/mL in 1XPBS for FACS (BD FACS Aria). The FACS gating scheme is represented in Figure 2.
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bioRxiv - Immunology 2022Quote: ... distasonis D13 strain and Bacteroides fragilis (ATCC® 25285) were cultured in anaerobic culture broth (Tryptic Soy Broth supplemented with 5 µg/ml Hemin (BD Biosciences) and 1µg/ml Vitamin K1 (VWR ...
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bioRxiv - Immunology 2022Quote: ... were picked from a selective plate and inoculated in 1 mL SD-UT medium [yeast nitrogen base-casamino acids (YNB-CAA) (BD Biosciences 223120) + 2% D-(+)-Glucose (Sigma G5767-500G ...
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bioRxiv - Developmental Biology 2019Quote: ... Passages were performed by resuspending Matrigel-embedded organoids in cold DPBS and transferring them to a Falcon tube using a 2 mL syringe with a 27 G ½ needle (BD Microlance #300635) to break them up ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were stained with 50 μg/ml propidium iodide and then analyzed by the flow cytometer (BD FACSCanto. San Jose, CA) using Flowing Software 2.5.0.
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bioRxiv - Biophysics 2019Quote: Fresh peripheral blood samples were collected by venipuncture into 6 ml lithium-heparinized Vacutainer® tubes (BD Vacutainer™, Franklin Lakes, NJ) from healthy female and male donors with informed consent as approved by the Columbia University Medical Center Institutional Review Board (IRB protocol no ...
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bioRxiv - Pathology 2020Quote: ... Approximately 9 mL of cord blood from gravidas chosen for this study was collected into anti-coagulant K2-EDTA tubes (BD Vacutainer 366643) containing a proprietary preservative.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Rats were sedated under isoflurane anesthesia and approximately 0.5 mL of blood was collected from a tail vein into a microtainer tubes with lithium heparin (BD, Franklin Lakes, NJ). Tubes were immediately centrifuged at 1000g for 10 min and the plasma supernatant was transferred into a labeled storage tube and frozen at −80°C until analyzed ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... rats were sedated under isoflurane anesthesia and tail-vein blood was collected in a volume of approximately 0.5 mL into microtainer tubes without additive (BD, Franklin Lakes, NJ). Tubes were immediately centrifuged at 1000g for 10 min and the serum supernatant was transferred into a labeled storage tube and frozen at −80°C until analyzed for heroin mid-point titer levels as described previously (Bremer et al. ...
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bioRxiv - Immunology 2021Quote: ... 106 cells were stimulated with 1µg/mL CMVpp65 overlapping peptides (JPT Peptide Technologies, Germany) in the presence of Brefeldin A (GolgiPlug (final concentration 5 µL/mL, BD Biosciences, USA) and Monensin (GolgiStop (final concentration 3.25 µl/ml ...
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bioRxiv - Immunology 2021Quote: Mouse splenocytes were cultured in RPMI-10 and stimulated with the S peptide pool at 1 µg/ml/peptide in the presence of GolgiStop™ and GolgiPlug™ (BD) for 6 hours ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... For start of the migration fresh cell culture medium was added with or without 10 μg/ml 9EG7 antibody (BD Biosciences, #553715). The progress of migration was recorded every minute with temperature and CO2 controlled Leica DM IL LED microscopes with Leica HI PLAN I 10x/0.22 air objectives ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency in presence or absence of 10 μg/ml 9EG7 antibody (BD Biosciences, #553715) for 24 h and scraped off manually with a rubber Corning Cell Lifter (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... and RNase A (80 μg/mL) for 30 minutes and then analyzed using a BD Accuri™ C6 flow cytometer (BD Biosciences). For quantitation of the mitotic fraction ...
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bioRxiv - Neuroscience 2019Quote: ... Drug solutions were IP injected in 0.1 ml solution/10 g of mouse using a fine needle insulin syringe (BD 324911, BD Bioscience). On average ...
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bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
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bioRxiv - Neuroscience 2020Quote: ... was drawn from a participant through venipuncture into a plastic whole-blood tube with spray-coated K2EDTA (16 x 100 mm x 10.0 mL BD Vacutainer® Plus). Whole blood was carefully layered on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... was plucked from each MOX plate and added to 5 mL of Brain Heart Infusion (BHI; BD Bacto, Franklin Lakes, New Jersey) broth and incubated at 25°C in a shaking water bath for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µL of PI (from 50 µg/ml stock solution) was then added and the cell cycle was analyzed by flow cytometry (BD FACSCanto™ II, BD Bioscience) using untreated cells as negative control for gating ...
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bioRxiv - Systems Biology 2022Quote: Approximately 6 mL of whole blood was collected at arrival into K3-EDTA glass blood tubes (BD Vacutainer; Franklin Lakes, NJ, USA) via jugular venipuncture ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA extraction and BrdU-labelled DNA immunoprecipitation were performed with anti-BrdU antibody (40 μL of 25 μg mL−1, BD Pharmingen, #555627). Validation of BrdU immunoprecipitation was carried out using qRT-PCR on known Early (BMP1 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Each culture contained a 10 μL 4.0 McFarland (McF) bacterial cell suspension (WHO-F or WHO-X) in 12 mL gonococcal (GC) broth supplemented with 1% IsoVitaleX (BD BBL™) and vancomycin ...
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bioRxiv - Microbiology 2021Quote: ... pseudintermedius ST71 in PBS was intradermally injected into the dorsal skin using 0.3 mL/31-gauge insulin syringe (BD, Franklin Lakes, NJ). At 1 h post-infection ...
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed the pellet was resuspended with 1 ml PBS and analyzed in a Flow Cytometer LSRFortessa (BD Biosciences, NJ, USA). Analysis of the data output was preformed via FlowJo v10.8 software using Watson model to calculate the percent of cells in each stage of the cell cycle.
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bioRxiv - Microbiology 2022Quote: ... HFK were maintained in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 µM Rho kinase inhibitor (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... HFKs were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 μM Rho kinase (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... These cells were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and with NIH 3T3 J2 fibroblast added as feeders ...
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bioRxiv - Developmental Biology 2019Quote: ... Then the cells were washed twice in 1 ml PBS with 10% FBS and examined on a BD FACSAria™ III cell sorter (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... Samples were resuspended to a volume 2.5 mL with PBS with 2% FBS and 0.4% EDTA and placed on an Imag (BD Biosciences, San Diego, CA) for 5 min ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... were pre-wetted with 15 μl of 35% ethanol for 1min prior to being coated with 50 μl of 10 μg/ml anti-porcine IFN-γ antibody (BD Biosciences) at 4ºC overnight ...
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged on plates pre-treated with 0.01% w/v poly-L-ornithine and coated with 10 ng/ml laminin (BD Biosciences, cat. 354232).
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bioRxiv - Cell Biology 2020Quote: ... 8 ml Vacutainer Cell Processing Tubes (both sodium citrate and sodium heparin-based tubes are acceptable; BD Biosciences; Franklin Lakes, NJ USA). Samples were processed within 24 hours after blood collection by collecting the PBMC-containing upper phase and washed with ice-cold PBS ...
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bioRxiv - Microbiology 2020Quote: RNA and virus injections of 5 and 10 μl were performed with insulin syringes (BD Micro Fine Plus, 1 ml, 30 G) into honey bee and bumble bee pupae respectively ...
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bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... onto a 40 µM nylon cell strainer and softly mashing the organ through the cell strainer into a petri dish using the plunger tip of a 1 ml syringe (BD Plastipak 303172). Cell suspensions were filtered several times and filters were rinsed with fresh buffer to obtain a homogeneous single-cell suspension ...
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bioRxiv - Molecular Biology 2022Quote: ... using a 23 G needle and a 1 ml syringe.Blood was collected into a serum separator tube (365967, BD, Franklin Lakes NJ), allowed to clot at room temperature for at least 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...