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Citations for Becton, Dickinson and Company :
1801 - 1850 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells (1 x 106/mL) were then recovered and activated for 3 days on plate-bound anti-CD3 (BD, #553058) and soluble anti-CD28 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... 1µm and 2µm) were added to 1 ml of PBS in 5mL round bottom polystyrene test tubes (BD Biosciences, UK) for flow cytometry and were run on a BD FACSVerse™ (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: Overnight cultures were grown with shaking at 37° C in 5 mL of BHI (BD Difco Bacto Brain Heart Infusion) broth ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then stained with PI (20µg/ml) for 10 minutes and were immediately analyzed on an Accuri C6 (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained daily at 1 x 106 cells ml-1 and collected for flow cytometry after staining for IgG1 and B220 (FITC anti-IgG1, BD Pharmingen ...
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bioRxiv - Physiology 2020Quote: ... stained with propidium iodide (PI, 10 µg/mL) and the number of PI negative (PI -ve) cells were determined by flow cytometry (BD FACSCanto II ...
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bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was transferred into a pre-coated 5 mL polystyrene flow cytometry tube and sorted using a 100uM nozzle in a FACSAria III Cell Sorter (BD Biosciences Inc. ...
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bioRxiv - Genomics 2022Quote: Approximately 3 ml of whole blood were collected into heparin-coated Vacutainer® blood collection tubes (BD Diagnostics, Oxford, UK) from all ten non-vaccinated control animals at -1 wk pre-infection ...
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bioRxiv - Pathology 2022Quote: ... Gas samples were collected and stored in 5-mL additive-free glass vacutainers® (BD Vacutainer® Z. ref: 367624) and analyzed by gas chromatography within two weeks of sample collection ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 12 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 24 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Biochemistry 2019Quote: ... shRNA expression was induced by doxycycline (2 μg/ml) and sorted using a BD FACSAria™ III (BD Biosciences-US) cell sorter to select for the high TurboRFP expressing cells ...
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bioRxiv - Immunology 2019Quote: Fresh breast tumor samples were cut into small pieces and gently triturated with a 5 mL syringe plunger on a 70 μm Cell-Strainer (BD) in RPMI-1640 medium (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: Single-cell suspensions of mouse mammary epithelial cells were blocked in Fc block cocktail (FACS buffer, 6.25 μg/mL Mouse BD Fc block (BD Bioscience) and 200 μg/mL Rat Gamma Globulin (Jackson ImmunoResearch) ...
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bioRxiv - Bioengineering 2019Quote: Blood was gently drawn from healthy volunteers who had not been subjected to any anticoagulant treatments for at least 2 weeks by standard venepuncture into sodium citrate vacutainers (2.7 mL, 3.2 % v/v sodium citrate; BD, NJ, USA). The informed consent was provided by each volunteer ...
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bioRxiv - Immunology 2019Quote: ... and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Microbiology 2019Quote: ... starter cultures for each Mtb strain were prepared by recovering a 20 μL aliquot from frozen stocks into a 10 mL volume of Middlebrook 7H9 broth (BD), supplemented with an albumin (Fraction V ...
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bioRxiv - Molecular Biology 2019Quote: ... that were either uncoated (unstimulated cells) or coated with anti-human CD3 (1µg/ml, clone UCHT1, no azide/low endotoxin, BD Bioscience) and anti-CD28 (10µg/ml ...
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bioRxiv - Genetics 2021Quote: ... and 10 μg ml−1 propidium iodide (Fluka) at 37°C for 30 min and analysed with a FACSCalibur (BD). Cell cycle distribution data were further analysed using ModFit LT 3.0 software (Verity Software House Inc).
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin) in a 48-well plate (Costar) coated with CD3 Abs (1 μg/ml; BD Biosciences, Clone UCHT1) and in the presence of soluble CD28 Abs (1 μg/ml ...
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bioRxiv - Systems Biology 2019Quote: ... washed in PBS and 250k cells were stained with PE-LDLR at 2 µg/ml (1:100, BD Bioscience 565653) for 20 minutes at 4°C and Zoombie NIR (BioLegend 423105 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were trypsinized using standard cell culture techniques and transferred into 5 mL polystyrene round-bottom tubes (#352052, BD Falcon). After PBS-washing and centrifugation (800 rpm ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 5 hours at 37C in the presence of brefeldin A (1 μl/ml, GolgiPlug, BD Biosciences), human recombinant IL-2 (10 U/well ...
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bioRxiv - Immunology 2020Quote: ... then washed and suspended to 3 million cells per mL in RPMI/0.05% HSA followed by data acquisition on a FACSVERSE (BD Biosciences). Basal fluorescence level for each sample was determined during the first ten seconds ...
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bioRxiv - Immunology 2021Quote: ... Bone marrow (BM) from femurs and tibias were flushed with defRPMI using 28G needle and 30 mL syringe (BD Medical). Cells were passed through a 70 μm nylon filter (Corning ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells were stimulated in the presence of plate-bound stimulator OKT3 at a final concentration of 2.8 μg/ml (mouse anti-human CD3 Clone OKT3 BD Biosciences). Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). PBMCs were stimulated or left unstimulated for 16hrs ...
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... and another 3 mL of blood was collected in tubes containing sodium heparin (Sodium HeparinN Plus, BD Vacutainer®, USA). Both were then centrifuged at 4,000 rev.min−1 for 10 min at 4º C ...
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bioRxiv - Microbiology 2019Quote: ... we incubated the coverslips or plates O/N at +4 °C with 0.1 mg/ml of collagen I from rat tail (BD Biosciences) in 0.25% acetic acid ...
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bioRxiv - Microbiology 2019Quote: Mtb strains were cultured at 37 °C in a biosafety-level 3 facility in 10 mL standing culture flasks containing 7H9 (Middlebrook 7H9 Broth; BD) with glycerol (0.2%) ...
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bioRxiv - Immunology 2019Quote: ... the cells were washed twice using ice-cold PBS and resuspended in PBS containing DAPI (0.5 μg/mL) and analyzed using an LSR II Fortessa (BD Biosciences). Post-acquisition data analysis was done using FlowJo 10 software ...
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bioRxiv - Genetics 2020Quote: ... we also collected 5-7 ml of peripheral blood in a tube containing the anticoagulant K3 EDTA (#6457, BD Biosciences) which was used for flow cytometry as described below ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in PBS stained with 10 µg/ml propidium iodide (PI) and sorted using the BD FACSMelody (BD Biosciences). Logarithmically growing “no drug” controls were collected after 2 hours of growth in drug-free YPD ...
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bioRxiv - Genomics 2021Quote: Each strain was cultivated for 3-7 days in 10 mL of Tryptic Soy broth (BD Diagnostic, catalog no. 211768) with 0.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pelleted and then resuspended in 2 mL of 0.5% BSA in D-PBS for cytometer-based sorting (FACSAria IIIu cell sorter, BD Biosciences). An aliquot of 200 µL stained cells was saved as input for analysis ...
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bioRxiv - Plant Biology 2020Quote: In vitro mycelium diameter measurements were performed by depositing a 5 μl droplet of a 1.5 × 105 spores ml−1 solution onto 1.5 % granulated agar (BD Difco™) plates supplemented with homogenates of WL- or WL+FR-treated leaves (1 gFW ml−1) ...
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bioRxiv - Developmental Biology 2021Quote: ... Explants were carefully detached from the cellulose membrane using a pair of forceps and a 1 mL syringe (329654, BD Vacutainer Labware Medical ...
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bioRxiv - Cancer Biology 2021Quote: ... After a wash and centrifuge the cells were suspended in DAPI (2.5 µg/mL) and subjected to FACS analysis (BD FACSAria™ Fusion flow cytometer, BD). Debris (by morphology plot ...
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bioRxiv - Immunology 2021Quote: ... at a final concentration of 2 μg/ml in the presence of monensin and costimulatory antibodies anti-CD28 and -49d (clones CD28.2 and 9F10, BD Biosciences) for 6 hours ...
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bioRxiv - Pathology 2021Quote: ... Blood was collected from the cranial vena cava via a 20 gauge needle and a 10 mL serum vacutainer tube (BD, with clot activator and gel for serum separation) ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... of the overnight cultures were transferred into 1.5 mL of LB media containing antibiotics in a 24-well microplate (cat# 353224, BD Falcon). The cultures were next grown at 37°C and 400 rpm using an incubating microplate shaker ...
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bioRxiv - Genomics 2020Quote: ... and a panel of 5 FITC-conjugated lineage antibodies (anti-CD41, anti-CD45R, anti-CD3e, anti-CD11b and anti-Ly-6G/6C, all at 1 μg/ml; BD 553848 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked with 3% (w/v) bovine serum albumin (BSA, ChemCruz) in PBS with 0.5 g/mL Fc Block (BD Biosciences) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... OD600 was taken and cultures were diluted to a concentration of 1⨯106 CFU/mL in cation-adjusted Mueller-Hinton Broth (caMHB) (BD). 0.05mL of diluted cultures were used to inoculate 96-well plates.