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Citations for Becton, Dickinson and Company :
1901 - 1950 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed by adding 200 μl of DNase-free water and resuspending them up and down using a 3 ml syringe and 0.5 mm needle (BD Biosciences). An equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in PBS containing 0.1 % BSA and 0.05 µg/mL propidium iodide (PI) and analyzed using a BD-FACS (BD Aria III). TelC-Alexa-488 PNA probe and PI nuclear staining was used in all flow-FISH analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS and resuspended in 200 µL of PBS with propidium iodide (2µg/ml) and analysed using flow cytometry (BD LSRFortessa, BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... A tissue suspension was constituted by adding 5 mL of Tumor & Tissue Dissociation Regent (TTDR, BD Bioscience, San Jose, CA), and then incubating the mixture at 37 °C for 30 min with frequent agitation ...
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bioRxiv - Genomics 2023Quote: ... The fresh tumor was minced into tiny cubes < 0.5 mm3 and transferred into a 15-mL conical tube (BD Falcon) containing 8 mL prewarmed HBSS ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were collected from a single soiled diaper and immediately placed in a tube completely submerged in 10 ml of thioglycolate broth (BD Difco™ Fluid Thioglycollate Medium Catalog # 225650) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no. I0634-1MG], and 2µg/mL GolgiPlug [BD Biosciences ...
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bioRxiv - Genetics 2023Quote: ... filter-sterilized YEPD was loaded into the AD-Chip channels at 20 µL/min using 20-mL syringes (BD Biosciences) driven by a KDS-230 pump (KD Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... 1x105 CFU bacteria were incubated for 48h at 37°C in 0.2 mL cation-adjusted Mueller-Hinton broth (CAMHB) (BD Biosciences) containing increasing concentrations of antibiotic (0.25 mg/L to 256 mg/L for Nafcillin and Oxacillin ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a serial dilution from 10 to 0.0006 µg/ml of either anti-CD81 (JS-81; 555675, BD Pharmingen), mouse IgG1 κ isotype control (107.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... were infected by administering 20 µl of the bacterial suspension (106 bacteria/injection) subcutaneously into the hind-leg footpad (Microfine Demi 0.3 ml syringes with a 30-G needle, BD #324826). As a vehicle control ...
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bioRxiv - Microbiology 2023Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Immunology 2023Quote: ... cells were cultured in complete media (RPMI 1640, 10% FBS, 1% Pen/Strep, Glutamax) supplemented with 1 μL/mL brefeldin A (Cat# 555029; Golgiplug, BD), 50 ng/mL phorbol myristate acetate (Cat# P1585 ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellets from each isolate were then resuspended in 1 ml of T-R minimal medium containing no carbon source and injected into the IsoCaRB system simultaneously using a 3 ml syringe (BD Biosciences #309657 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (3 × 105) in 300 mL of serum-free medium were seeded into FluoroBlock TM Cell Culture inserts (BD Falcon). Serum free media with 100 ng/mL LOX ...
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bioRxiv - Cell Biology 2023Quote: ... Blood was collected via venipuncture with a 21-gauge needle into four 10 ml Acid Citrate Dextrose-A (ACD-A) vacutainers (BD Vacutainer ...
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet was resuspended in 1 ml of 1X PBS and 1 μl of Fixable Viability Stain 780 (BD Horizon) and incubated for 5 min at 37°C protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with 4 µg/ml propidium iodide (PI) and viability was analyzed with BD FACSCanto II (BD Biosciences) as a percentage of propidium iodide-positive CFSE-positive cells.
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bioRxiv - Biochemistry 2024Quote: ... Cells were blocked with 0.1 mg/ml mouse IgG prior to staining with BV-421 labeled anti-human CCR6 antibody (Clone 11A9, BD Biosciences) for 1 h at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Microbiology 2024Quote: ... and 50 μg/mL pantothenate (Beantown Chemical, Hudson, NH) either in a rotator (10 rpm) or on plates with 7H10 agar (BD) and the above additives ...
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bioRxiv - Microbiology 2024Quote: ... BALF was collected by pumping 1 ml of sterile PBS into the lungs and recovered with a syringe (BD #309659). This process was repeated once to collect 2 mL of BALF.
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 5 hr with and without 50ng/mL phorbol 12-myristate 13 acetate (PMA) and 1μg/mL ionomycin to stimulate cytokine production in T cells as well as GolgiPlug (BD Biosciences) to retain cytokines in the cytoplasm ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of bacterial suspension in PS (1 x 105 bacteria) were injected in the last left proleg of the larvae using 0.3 ml insulin syringes (BD MicroFine). One group of 10 larvae was inoculated per condition ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained for 30Lmin on ice using the following antibodies (all at 5Lμg/mL): anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-CD11c-PerCP-Cy5.5 (clone N418 ...
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bioRxiv - Immunology 2024Quote: Splentocyte samples were either aliquoted in mFACS Buffer for staining or resuspended in mR10 and stimulated ex vivo with LCMV peptide DbGP33 (0.2 μg/mL) in the presence of Golgi Plug and Golgi Stop (BD Bioscience) for 5 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and resuspended the cells in 0.3 ml of cold PBS + RNase inhibitor for sorting in a FACS Melody cell sorter (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... 5×104 alive GAFs or glioma cells were incubated with PE Mouse anti-Human CD105 (2µg/mL, BD Biosciences #560839) and APC Mouse anti-Human CD90 (2µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: Female 6-week-old nu/nu mice were injected subcutaneously into the flank with 106 breast cancer cell lines (HS578T or ZR-751) in 0.1 ml 30% Matrigel (BD Biosciences)/PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions (1.5 x 104 cells per ml) were plated in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 100μl of this mix were added per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... and cells collected from the wells were washed and stained with propidium iodide (2 µg/ml) to distinguish live from dead and run on a flow cytometer (BD FACSCelesta).
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... Both ends of the femur were cut and 10 mL RPMI media was passed through the bone with a 25G needle (BD Biosciences). The supernatant was centrifuge at 600 x g for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in staining buffer containing 0.5 µg/mL propidium iodide and sorted into CD90lo and CD90hi fractions using a FACSAria™ Fusion cell sorter (BD Biosciences). Sorted cells were collected in the complete medium and processed for total RNA isolation immediately after sorting.
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bioRxiv - Cell Biology 2022Quote: Transduced cells were selected with 10 µg/mL Blasticidin (Thermo) and thereafter sorted in 96 well-plates with a BD FACS Aria III (BD Biosciences) (Imaging Facility ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells were stimulated with plate-bound anti-CD3 (LOT: 4310627, eBioscience) and 1.5 μg/ml soluble CD28 monoclonal antibodies (Cat: 557393, BD biosciences pharmingen) in DMEM medium and cytokines (IL-6 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... then cultured in complete RPMI media (3×106 cells/mL) plated on anti-CD3 (145-2C11; BD Pharmingen, San Diego, CA) antibody-coated tissue culture plates ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences). For subsequent limiting dilution cloning ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACSAria2 or FACSAria3; BD Biosciences).
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bioRxiv - Genomics 2019Quote: ... one aliquot of cells was resuspended in 2 ng/mL propidium iodide for flow cytometry analysis using a FACSCalibur flow cytometer (BD Biosciences). For breakpoint detection ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...