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Citations for Becton, Dickinson and Company :
2051 - 2100 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... purified NK cells were incubated for 10 minutes at room temperature with 2.5µg of Human BD Fc Block followed by addition of 10μg/mL of anti-NKG2D purified antibody (BD Biosciences, clone 1D11) or isotype control antibodies prior to addition of SVG-A cells ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Systems Biology 2023Quote: ... all transformants were cultured individually in 96-well plates in 1 ml of the appropriate minimal liquid media (AD: -Leu; BD: -Trp) at 30°C until saturated (∼36-48 hours) ...
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bioRxiv - Neuroscience 2023Quote: Cells were directly sorted into 1.5 ml tubes containing 100 µl of extraction buffer (XB, Arcturus PicoPure RNA isolation kit) with a FACSAria III SORP (BD Biosciences) flow cytometer equipped with a 90 µm-diameter nozzle ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Immunology 2023Quote: ... and the 2D effective affinity AcKa was estimated by normalizing
against mr and ml that were measured using PE-labeled monoclonal antibody together with QuantiBRITE PE standard beads (BD Biosciences): -
bioRxiv - Immunology 2023Quote: ... and CD4+FYP+ Treg cells at indicated Treg : Tresp ratios in the presence of 1 μg/ml anti-CD3 antibody (BD Pharmingen). After 3 days ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then the resulting 350 μL volume was drawn twice into a 1 mL luer slip tip tuberculin syringe (BD Catalog # 309659) fitted with a 27G-½” needle ...
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bioRxiv - Immunology 2023Quote: ... Cell viability was measured via PI (1-2 μg/mL) uptake and flow cytometric analysis on an BD LSR-Fortessa X20 or BD FACSCanto instrument using FACSDiva software (BD Biosciences). Data were analyzed using FlowJo software version 10.6.1 or WEASEL software.
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were then harvested and incubated in a blocking buffer containing 10%FBS and 1μg/mL Human Fc block (BD Biosciences, USA) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were stimulated with S peptides (5μg/ml) or medium as negative control for 12h at 37℃ before a 6h treatment with brefeldin A (BD Pharmingen, 554724). Cells were collected and stained with antibodies to mouse CD45 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells from 2-month-old C57BL/6 WT mice were sorted using FACS and resuspended in culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with EGF (10 ng/ mL, BD Bioscience), Rspo1(250 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells were collected from FACS and cultured in 200 μL culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with 10 ng/ mL EGF (BD Bioscience), 250 ng/ mL Rspo1(R&D) ...
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bioRxiv - Plant Biology 2023Quote: Bacterial activation of Pseudomonas fluorescens strain UM270 was carried out by removing a hoe from the bacteria and placing it in a flask with 500 mL of Nutrient Broth (BD BIOXON), keeping it under constant agitation at 120 rpm at 28 °C for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... and overlaid on a base layer containing 1.5 ml RPMI growth medium with 0.5% Difco Agar Noble (Becton, Dickinson and Company) in six-well plate wells ...
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bioRxiv - Microbiology 2023Quote: ... The resulting colonies were inoculated into 5 mL of marine broth medium (BD Difco Marine Broth 2216; Becton, Dickinson and Company), which was shaken at 300 rpm and 25°C until bacterial growth was confirmed ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered using 5 mL polystyrene round-bottom tubes with cell-strainer caps (Falcon) before sorting and cytometry on FACSAria III Cell Sorter (BD Biosciences). For sorting ...
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Microbiology 2023Quote: ... and then 10 mL was dispensed into each sterile tissue culture dish (BD Falcon, 100 mm diameter x 20 mm depth) and incubated at 37°C with 5 % CO2 for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... at 9:00 am 5 mL of fasted blood from the antecubital vein was collected into an EDTA Vacutainer tube (BD, 366643), then centrifuged at 800 x g for 10 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The cOBBs were resuspended in 1:1 v/v ECM gel to cOBBs and transferred to a 1 mL disposable syringe (BD Biosciences). The cOBBs were centrifuged at 100g for 3 min and the supernatant removed ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was drawn from healthy volunteers (IRB study #BMB-08-012) into 10 mL CTAD tubes (citrate-based anticoagulant containing the platelet inhibitors theophylline, adenosine, and bipyridamole, BD Vacutainer). Samples were maintained at 4°C and processed within 72 h of collection.
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bioRxiv - Bioengineering 2023Quote: ... Cells were then selected in puromycin (1μg/ml) 2 days after transduction and sorted for their EGFP fluorescence after 6 additional days using FACS ARIA III (BD Biosciences). Cells were grown for recovery and collected for genomic DNA extraction with DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Immunology 2023Quote: ... 1 μg/ml each of ESAT-6 and CFP-10 peptide pools (provided by Aeras, Rockville, MD) or R10 media for 2 h before adding 10 μg/ml BD GolgiPlug (BD Biosciences) for a further 12 hours ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the cells in 0.6 ml of cold PBS + RNase inhibitor and then filtered the cells with 100-μm cell strainers (Falcon brand, BD Biosciences). Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were inoculated subcutaneously in the right hind flank with 0.1 mL of a 50% RPMI-1640 / 50% Matrigel™ (BD Biosciences) mixture containing a suspension of A549 tumor cells (approximately 5×106 cells/mouse) ...
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bioRxiv - Microbiology 2023Quote: Giardia lamblia trophozoites (strain WBC6, ATCC 50803) were grown at 37°C without shaking in 16-ml screw-cap plastic tubes (BD Falcon) containing 12 ml modified TYI-S-33 medium supplemented with bovine bile ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were supplemented with FACS buffer containing DAPI in a final concentration of 50 ng/mL and analyzed using a LSRFortessa™ SORP (BD). Data was analyzed using FlowJo (BD) ...
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bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... The PDMS chip (soaked in PBS) and the culture medium (filled in 10 mL syringes; BD Biosciences, 300912, diameter 14.5 mm) were degassed before use for at least one hour in a vacuum desiccator chamber.
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Genomics 2024Quote: ... Blood of three healthy donors with available whole genome sequencing data was collected in 10 ml K2EDTA blood collection tubes (BD Vacutainer), and plasma was isolated by two centrifuge steps as described in (Marcozzi et al ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-MPO-FITC (clone M1/70, catalog no. 553310; BD Biosciences), Fc Block ...
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bioRxiv - Immunology 2023Quote: ... The PBMC layer was transferred to a 50 mL conical tube and red blood cells lysed with Pharm Lyse buffer (BD 555899) for 10 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...