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Citations for Becton, Dickinson and Company :
2001 - 2050 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: One day before VBP re-seeding (Day 9), collagen constructs were prepared (15, 41) by combining high-concentration rat tail collagen (4mg/ml; 80% final volume BD Biosciences) and 10xDMEM (10% final volume ...
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bioRxiv - Bioengineering 2020Quote: ... 0.8-1 ml of the diluted bacterial cells were slowly injected into the apical channel through the injection port (Figure 1) using a 1-ml syringe (309659, BD Biosciences) with a needle (305127 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Cell Biology 2020Quote: ... the cell invasion assay was performed using Transwells (8 μm pore size, millipore) with inserts coated with Matrigel (50 mg/ mL, BD Biosciences). 786-O and ACHN cells (1.0 × 105 cells/well ...
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bioRxiv - Immunology 2020Quote: ... cells were prepared as described for flow cytometry and then sorted into FBS containing media (RPMI 1640, 20% FBS, 1% HEPES, 100 mg/mL penicillin/streptomycin) on a FACSAria II (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Immunology 2019Quote: Cell populations of interest were sorted into 1.5 ml reaction tubes containing 200 μl FACS-buffer using a BD FACSARIA III (BD BioSciences, USA). The cell suspension was centrifuged onto SuperFrost Plus glass slides (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... To measure IFNγ secretion cells were incubated overnight in complete RPMI supplemented with 100 ng/ml SIINFEKL with and treated with Golgi Stop (BD Biosciences) 4 hours before intracellular staining and flow cytometry analysis ...
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bioRxiv - Immunology 2019Quote: ... For anti-Fas-induced death cells were plated similarly except in the presence of anti-Fas (2μg/ml) (BD cat # 554254) and protein G (2μg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... This strain was grown overnight from a frozen stock (25% glycerol) in 3 mL MRS medium (DifcoTM Lactobacilli MRS Broth, BD #288110) containing 10 µg/mL chloramphenicol ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Immunology 2020Quote: ... for 30 min prior to the addition of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences), brefeldin A (1 μl/ml ...
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bioRxiv - Immunology 2020Quote: ... in the presence of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences) and IL-2 (10 IU/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were passed through a 40 μm filter and stained with 2 μg/mL DAPI before sorting on a BD FACSARIA III (BD Biosciences). Aliquots of 40,000 nuclei were collected in 10X binding buffer (200 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Plant Biology 2020Quote: ... and diluted to a final concentration of 1.5 × 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to inoculation ...
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bioRxiv - Systems Biology 2021Quote: ... vaginal swabs and 10 ml blood samples were collected in red-top vacutainer tubes (Becton, Dickinson and Company, New Jersey, USA) from the dam(s ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of the cell suspension (more than 1×105 cells) was transferred to a 5 mL FACS tube (BD Biosciences) and stained in duplicate with 10 μL of FITC Annexin V conjugate and 10 μL of propidium iodide (PI ...
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bioRxiv - Immunology 2021Quote: ... cells were previously stimulated with 100 ng/ml of PMA and 1 μg/ml of Ionomycin for 1h at 37°C and then incubated in GolgiStop and GolgiPlug (both from BD PharMingen) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The suspended cell lysates were pipetted 6 times gently and passed through a syringe 20 times (1 ml TB Syringe, BD, USA), and avoid any bubble in the procedures ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with 2 × 2 ml Pharmingen stain buffer and fixed with 4.21% (w/w) formaldehyde (BD Biosciences, #554655). Cells were analyzed on LSRFortessa X-20 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Biophysics 2020Quote: ... and a strain with a known binding site and repressor copy number (R) - were inoculated into individual tubes with 2 mL of Lysogeny Broth (LB Miller Powder, BD Medical) with 20 µg/mL of chloramphenicol and 30 µg/mL of kanamycin ...
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bioRxiv - Molecular Biology 2021Quote: ... coli cells (100 μl) were thawed on ice and transferred into a chilled 14 ml polypropylene round-bottom tube (BD Biosciences). Then 50ng of the desired plasmid DNA was added to the E ...
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bioRxiv - Immunology 2020Quote: For data acquisition on the BD FACSymphony the following amounts of 14 different fluorescent streptavidin conjugates were added to 10 μl of pMHC monomer (100 μg/ml): 1 μl of SA-BB790 (BD, custom), 1 μl of SA-BB630 (BD ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Immunology 2020Quote: ... stimulated with 1 μg/ml LCMV NP118-126 peptide for 1 hour and then in the presence of GolgiPlug (BD Biosciences) and peptide for an additional 4 hours ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2022Quote: Single cells suspensions were incubated a mixture containing 50 µg/ml rat anti-mouse CD16/CD32 (BD Biosciences, Franklin Lakes, USA), normal Syrian hamster serum (1:100 ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... was coated with Cell-Tak® (22.4 μg/ml in 0.1 M NaHCO3; #734-1081; BD Biosciences, San Jose, CA, USA) at 37°C (not CO2 corrected ...
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bioRxiv - Immunology 2022Quote: ... OD values were converted to ng/ml of IgA by comparison with a purified IgA standard (BD Biosciences, San Jose, CA). Fecal extracts from individual mice were obtained as previously described (41 ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Microbiology 2022Quote: ... 229E and uninduced sample as a control) were grown to 80% confluency then induced with 1ug/ml of Doxycycline (BD Biosciences). After 24hr cells were collected in TRIzol Reagent and RNA was harvested following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
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bioRxiv - Microbiology 2022Quote: ... Colonies from each strain were suspended in 9 ml of sterile 0.1% peptone water (BD, cat. no. 218071, Franklin Lakes, NJ) and adjusted to an optical density at 600 nm of 0.13 (∼108 CFU/ml) ...
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bioRxiv - Immunology 2022Quote: ... Protein transport inhibitor containing Monensin and protein transport inhibitor containing Brefeldin A were added to cells (both 10 μg/ml, BD Biosciences) and cells cultured for an additional 4 hours ...
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bioRxiv - Immunology 2022Quote: ... human cells were stimulated with PMA and ionomycin (500 ng/mL, eBioscience) in the presence of GolgiPlus and GolgiStop (BD Biosciences) for 4 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or the TAPI (prepared as described above) was administered directly into the pocket via a 1 mL syringe (BD, cat# 309628). The implants ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
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bioRxiv - Immunology 2023Quote: ... and the 2D effective affinity AcKa was estimated by normalizing
against mr and ml that were measured using PE-labeled monoclonal antibody together with QuantiBRITE PE standard beads (BD Biosciences): -
bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...