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Citations for Becton, Dickinson and Company :
2151 - 2200 of 2276 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2022Quote: ... cells were spun at 500xG and resuspended in Flt3L DC Buffer (T-cell buffer + 10% B16-Flt3L conditioned RP10G medium + 1000U/mL Mouse GM-CSF [BD Biosciences, #554586]). Dendritic cells were plated in a total volume of 75 μL and rested overnight ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Immunology 2023Quote: ... cells were seeded in 24-well plates or CELLview Cell Culture Dish with four compartments) (USA Scientific, 5662-7870) in culture media with 1 μg/ml propidium iodide (PI, BD Bioscience, 556463) followed by calculating PI positivity on either an Inverted Nikon Ti2 fluorescence microscope with a 40X objective (N.A ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS and resuspended in 200 µL of PBS with propidium iodide (2µg/ml) and analysed using flow cytometry (BD LSRFortessa, BD Biosciences).
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bioRxiv - Genetics 2023Quote: ... Primed H9 cells were maintained in HFF-conditioned media (HFF-CM) supplemented with 8 ng/ml of β-FGF (MILTENYI BIOTE) on Matrigel (BD, Biosciences) coated surfaces cell lines in a humidified 5% CO2 incubator at 37ºC ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in Tfh conditioning media (RPMI complete media with 10% fetal bovine serum, 10μg/ml anti-IL4 (BD Biosciences, clone 11B11), 10μg/ml anti-IFNγ (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ produced upon re-activation of peptide-specific T cells and captured on anti-IFNγ capture antibody was visualized via sequential addition of 2 μg/mL biotinylated anti-IFNy detection antibody (BD #51-1818KA), Streptavidin-Horseradish Peroxidase (HRP ...
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bioRxiv - Immunology 2023Quote: ... relatives and healthy donors was collected by venipuncture at the antecubital area into 9 mL Vacutainer ACD-A collection tubes (BD; cat # 455055) and processed within 24 hr of collection ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Immunology 2023Quote: Neutrophilic airway inflammation was induced by subcutaneous injection of 5μg of CA emulsidied in 100μl of CFA containing 5mg/ml H37Ra (BD Difco, Franklin Lakes, NJ). Intranasal CA challenge was performed as described above ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa was maintained on TSA plates at 4°C and an inoculum was prepared by transferring a touch from this plate into 10 mL of tryptic soy broth (BD Bacto™) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-CD28 antibodies (eBioscience, clone 37.51, 1 µg/ml) on TC plates pre-coated with anti-hamster antibody (BD Pharmingen, G94-56), and IL-2 (Immunotools ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Cell Biology 2023Quote: ... 25,000 cells were suspended in 1.5 ml RPMI growth medium with 0.35% Difco Agar Noble (Becton, Dickinson and Company, Franklin Lakes, NJ) and overlaid on a base layer containing 1.5 ml RPMI growth medium with 0.5% Difco Agar Noble (Becton ...
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bioRxiv - Cancer Biology 2024Quote: ... and with either CSF-1R blocking antibody or isotype control antibody at 50 ng/mL (cat# NBP1-43363, clone AFS98, Novus Biologicals; IgG K isotype control cat# 554682, BD Pharmingen). After 24 hours incubation ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
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bioRxiv - Neuroscience 2020Quote: ... and then were stamped with a 1:1 mixture of rattail collagen (final concentration 1.0 mg/mL, BD Biosciences, San Jose, CA, USA) and poly-D-lysine (final concentration 0.25 mg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The seeded cells (5 x 104 cells/mL) were pre-stained with 1 μL Orange NO probe (BD Biosciences, San Jose, CA, USA) per 500 μL cell suspension for 30 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... the embryos were washed three times in PBS and then about 200 embryos were transferred into a 15 mL centrifuge tube (BD Falcon™ Tube) with 10mL 0.25% trypsin ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μl of the bacterial inoculum was transferred from a Hungate tube to under oil in the eppendorf tube using a syringe (1 ml, Luer-lok Tip, BD Biosciences, REF 309628). Bacteria were added to the media above the colon monolayer by pipette at 1:20 v/v ratio (2 μl bacteria:40 μl media ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were stained with 10 μg/ml propidium iodide (PI) staining solution from FITC Annexin V Apoptosis Detection Kit (BD Pharmingen; Cat#: 556547) and 0.2 mg/mL RNase A (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 mL of each milt sample was diluted 1:9 in Storfish (IMV Technologies, France; an inactivating medium) in 15 mL tubes (Falcon, BD Biosciences, Allschwil, Switzerland). These tubes were then placed on ice and are referred to as ‘diluted milt’.
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bioRxiv - Developmental Biology 2020Quote: Single-cell suspensions of mouse mammary epithelial cells were blocked in Fc block cocktail (FACS buffer, 6.25 μg/mL Mouse BD Fc block (BD Bioscience) and 200 μg/mL Rat Gamma Globulin (Jackson ImmunoResearch) ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Cancer Biology 2020Quote: ... Infected cells were selected with 1 μg/ml puromycin and isolated by FACS using a FACS-Aria II cell sorter (Becton Dickinson, BD, Franklin Lakes, USA) based on TurboRFP expression after the induction with 1 µg/ml of Doxycycline
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... A further 8 mL of blood was collected in tubes containing Clot activator and gel for serum separation (SST II Plus, BD Vacutainer®, USA), and another 3 mL of blood was collected in tubes containing sodium heparin (Sodium HeparinN Plus ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... approximately 200 μL cells were added into the flow tube containing 2 mL PBS and tested by the flow cytometry (BD, New Jersey, USA). Three independent assays were performed.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... segments of the aorta using a PE-50 heparinized saline-filled (50 IU/mL) cannula (ID 0.58 mm, OD 0.965 mm; BD Diagnostics, Sparks Glencoe, MD, USA). Both arterial pressure signals were simultaneously monitored and recorded via an attached pressure transducer (Transonic SP200 Pressure System ...
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bioRxiv - Immunology 2020Quote: ... Isolated LNMCs were incubated with SIV peptide pools covering gag and env in the presence of co-stimulatory αCD28 and αCD49d (BD, 10 μg/mL) on goat-α-mouse IgG F(ab)2-coated (KPL ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Immunology 2020Quote: ... cells were exposed to IFNγ (20ng/mL for 24 hours) and sorted to >99% knockout purity (PD-L1 negative) using fluorescence-activated cell sorting (BD Aria III sorter).
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bioRxiv - Immunology 2020Quote: ... and subsequently PBMCs were stimulated in R10 media with specific peptide pools or medium alone (DMSO control) in the presence of 1 μg/ml of α-CD28 (BD bioscience 555725) and α-CD49d (BD bioscience 555501 ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed in cold DPBS/BSA containing 2,000 U/mL DNase I and successively passed through 100 μm and 40 μm mesh filters (BD Biosciences, San Jose, CA) to yield a single-cell suspension.
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bioRxiv - Immunology 2022Quote: ... 8 mLs of peripheral blood was drawn into each of two BD Vacutainer Cell Preparation Tubes with sodium citrate (BD Biosciences catalog #362760). The tubes were centrifuged at 1,800 x g for 30 minutes at room temperature (RT) ...