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Citations for NanoTemper :
1151 - 1200 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... After incubation the samples were quickly spun down and loaded into Monolith NT.115 Standard capillaries (NanoTemper Technologies) at room temperature (~25 °C) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fnorm measurements were obtained with NTA Analysis software (NanoTemper Technologies) and normalized (ΔFnorm ...
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bioRxiv - Synthetic Biology 2020Quote: ... MST measurements were performed using a Monolith NT.115 Green Red MST instrument (Nanotemper technologies, München, Germany) with MST grade standard treated NT.115 capillaries ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Pre-dialyzed protein and mutant samples were diluted (∼0.5 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: The melting temperature (T m) of sE2 (1mg/ml) was calculated using the Prometheus NT.48 (Nanotemper, Munich, Germany) during heating in a linear thermal ramp (1°C ...
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bioRxiv - Biophysics 2020Quote: ... Data was analyzed using the analysis program provided by Nanotemper Technologies ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Ton and Tm were determined automatically using PR.ThermControl (NanoTemper). All experiments were performed in duplicates.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: Melting profiles were acquired using a Prometheus NT.48 (NanoTemper). Experiments were performed using standard capillaries and a sample volume of 12 μl per capillary ...
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bioRxiv - Molecular Biology 2020Quote: ... L765F and L765A point mutants in NMR buffer were assessed by nanoDSF using a Prometheus NT.48 instrument (Nanotemper). Samples were heated from 15 °C to 75 °C on a gradient increasing by 2 °C per minute at 30% excitation.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microscale thermophoresis measurements were performed on the Monolith NT.115 (NanoTemper Technologies) using fluorescently labelled DVD-actin ...
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bioRxiv - Biochemistry 2020Quote: Thermal unfolding of wild-type and C88A GDAP1Δ303-358 in SEC buffer was studied by nanoDSF using a Prometheus NT.48 instrument (NanoTemper). The fluorescence of tryptophan was excited at 280◻nm and recorded at 330◻nm and 350◻nm ...
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bioRxiv - Biochemistry 2020Quote: ... Microscale thermophoresis was carried out using the Monolith NT 115 instrument from Nanotemper technologies [33] ...
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bioRxiv - Microbiology 2020Quote: ... Samples were loaded into standard glass capillaries (NanoTemper Technologies, MO-K022). Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were loaded into standard glass capillaries (NanoTemper Technologies, MO-K022). Microscale thermophoresis was completed in three independent experiments on a Monolith NT ...
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bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
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bioRxiv - Microbiology 2020Quote: ... Monolith His-Tag Labeling kit (MO-L018) was from NanoTemper Technologies.
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bioRxiv - Microbiology 2020Quote: ... 115 instrument (NanoTemper Technologies) running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
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bioRxiv - Microbiology 2020Quote: ... as recommended by the Monolith His-Tag Labeling Kit RED-tris-NTA protocol (NanoTemper Technologies, MO-L008). The binding buffer used for reactions [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Biochemistry 2020Quote: ... Data analysis was carried out using NTanalysis (Nanotemper technologies, Munchen, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Using a capillary set (Nanotemper technologies), the samples were loaded one by one into the machine ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: Binding affinities were measured using the Monolith NT.115 (NanoTemper Technologies, GmbH). Proteins were fluorescently labelled with Atto488 according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 115 Pico (NanoTemper Technologies) at 25 degrees C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Measurements were performed on a Monolith NT.115 instrument (NanoTemper Technologies GmbH) at 25°C with 3 s / 22 s / 2 s laser off/on/off times and continuous sample fluorescence recording in premium capillaries and using an excitation power of 90% and a high MST power mode ...
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bioRxiv - Biophysics 2020Quote: The thermal stability of target proteins in different buffers and the potential stabilizing effects of ligands was evaluated at 1 μM using a direct thermal shift assay using a Tycho NT6 instrument (Nanotemper Technologies), monitoring intrinsic protein fluorescence at 330 and 350 nm during a thermal ramp from 35 °C to 95 °C ...
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bioRxiv - Biophysics 2020Quote: ... MST analysis was carried out using premium-coated and standard treated capillaries (NanoTemper), and buffer matching the SPR conditions (HEPES 10 mM ...
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bioRxiv - Biophysics 2020Quote: ... were fluorescently labelled following the manufacturer protocol for free amine coupling of the dye (RED-NHS 2nd Generation (amine reactive)) (NanoTemper) to lysine residues ...
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bioRxiv - Biophysics 2020Quote: ... The protein concentration was additionally measured on ND-1000 spectrophotometer (NanoDrop®) and the protein quality was evaluated by nanoDSF using a Tycho (Nanotemper).
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bioRxiv - Biophysics 2020Quote: ... Thermophoretic experiments were conducted using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Briefly ...
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bioRxiv - Biophysics 2020Quote: The thermophoretic measurements were performed using Monolith NT.115 device with red detection channel (NanoTemper Technologies, Munich, Germany). Recombinant Spikeplug was produced in our laboratory ...
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bioRxiv - Biophysics 2020Quote: ... The assay was performed in duplicate and the values reported were generated through the usage of MO Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Cell Biology 2020Quote: Microscale thermophoresis experiments were performed using a Monolith NT.115 from Nanotemper Technologies (Munich ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Microbiology 2020Quote: Prometheus NT.48 NanoDSF instrument (NanoTemper Technologies) was used for all the Tm determination experiments ...
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bioRxiv - Microbiology 2020Quote: Thermal melting profiles were measured by using a Tycho (NanoTemper), following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... KD determination was performed using the MO.Affinity Analysis (NanoTemper, Germany) software version 2.3 ...
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bioRxiv - Microbiology 2020Quote: Thermal denaturation was performed on the Prometheus NT.48 (Nanotemper) from 20 to 95°C with 80 % excitation laser power and 2 °C/min of heating rate ...
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bioRxiv - Cancer Biology 2020Quote: ... ThermControl software (NanoTemper Technologies). Raw data was exported for plotting the thermal stability curve in GraphPad Prism.
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently loaded into the Prometheus NT.48 device (NanoTemper Technologies, Germany). Samples were heated from 20 °C to 95 °C with a slope of 1 °C/min and the unfolding transition temperatures were automatically identified by the PR ...
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bioRxiv - Biophysics 2020Quote: ... pH 8.2) was mixed with 10 µl of 300 µM NT647-N-hydroxysuccinimide fluorophore (NanoTemper Technologies) and incubated in the dark for 30 min at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then loaded into sixteen premium-coated capillaries (NanoTemper Technologies) and fluorescence was recorded for 20 s using 100% laser power and 40% MST power ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biophysics 2020Quote: ... and loaded into a Prometheus NT.48 device (Nanotemper) controlled by PR.ThermControl (version 2.1.2) ...
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bioRxiv - Molecular Biology 2020Quote: Purified SecB was randomly labelled using an NT-647-NHS labelling kit (Nanotemper Technologies). 160 nM NT-647-labelled SecB was incubated with the indicated concentrations of unlabelled AscA or AscAΔMBD in buffer (20 mM potassium HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2020Quote: ... The thermophoretic properties were of the labelled SecB were then determined with a Monolith NT.115 (Nanotemper Technologies) using MST Premium Coated Capillaries at 100% MST power ...