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Citations for NanoTemper :
951 - 1000 of 1145 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... A Monolith NT.115 instrument (NanoTemper Technologies) was used with a 50% LED power and laser pulses at both 60% and 100% power ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The thermophoresis signal was then measured on a Monolith NT.115 device (Nanotemper) with premium capillaries (MO-K025) ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Biochemistry 2020Quote: Using the Prometheus NT.48 instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... 2.3 (NanoTemper Technologies).
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bioRxiv - Biochemistry 2020Quote: The microscale thermophoresis (MST) assay was performed using a Monolith NT.115 instrument (NanoTemper Technologies). For the MST assay ...
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Biochemistry 2020Quote: ... and measured in duplicates in a Prometheus NT.48 (NanoTemper) with the following settings ...
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bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... Microscale thermophoresis assays were performed on a NanoTemper ® Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany). Samples were prepared in 20mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biochemistry 2020Quote: Microscale thermophoresis was performed using a Monolith NT.115 from Nanotemper Technologies (Munich ...
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bioRxiv - Biochemistry 2020Quote: ... Experiments were performed in triplicates and data were fitted using the Kd model with the MO.Affinity Analysis software (Nanotemper Technologies, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Biochemistry 2020Quote: ... the SpaKC sample was loaded to column B (Nanotemper MO-L011) and eluted with 450 μL of assay buffer (50mM Na2CO3/NaHCO3 ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were then loaded into Monolith NT.115 capillary (Nanotemper Technologies) and measured by using 20% (Auto-detect ...
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bioRxiv - Biochemistry 2020Quote: ... the binding affinity between SpaKC and SpaAleader was measured using Monolith NT.115 Pico (Nanotemper Technologies). SpaKC was fluorescently labelled by the labeling kit (Monolith Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive) ...
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bioRxiv - Plant Biology 2020Quote: ... The intrinsic fluorescence signal was measured as a function of increasing temperature with a Prometheus NT.48 fluorimeter (NanoTemper Technologies), with 55% excitation light intensity and 1 °C/min temperature ramp ...
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: The binding of W25 and the structural integrity of Spike RBD was verified using a label-free thermal shift assay with Tycho NT.6 (Nanotemper Technologies GmbH) using intrinsic tryptophan and tyrosine fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... W25 was fluorescently labeled with the Monolith Protein Labeling Kit RED – NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH) following the protocol established in the manual ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity measurements were conducted in Premium Capillaries Monolith NT.115 (MO-K025, NanoTemper Technologies GmbH) and repeated three times ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity Analysis v.2.3 (NanoTemper Technologies, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... and the Monolith NT.115 Premium Capillaries (NanoTemper Technologies, Germany).
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bioRxiv - Molecular Biology 2020Quote: The MST experiments were performed in a Monolith NT.115 Pico (NanoTemper Technologies, Germany) using the Monolith Protein Labeling Kit RED-NHS (Amine Reactive) ...
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bioRxiv - Microbiology 2020Quote: ... NanoDSF profiles were measured with a Tycho system (Nanotemper). The first derivative profiles were then fit as a sum of Gaussian curves in Excel (Microsoft).
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bioRxiv - Biophysics 2020Quote: ... Measurements at 80°C required capillary sealing (Capillary Sealing Paste and Capillary Sealing Applicators; NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
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Identification of glutamine synthetase as a novel contryphan-Bt binding protein by his-tag pull downbioRxiv - Pharmacology and Toxicology 2020Quote: Microscale thermophoresis (MST) experiments were conducted on a NanoTemper Monolith NT.115 system (NanoTemper, Germany). Recombinant human glutamine synthetase (GS ...
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bioRxiv - Biophysics 2020Quote: ... 115 Pico (NanoTemper Technologies) at 25°C ...
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bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biophysics 2020Quote: Binding experiments were performed using a Monolith NT.115 Microscale Thermophoresis (MST) instrument (NanoTemper) at room temperature in phosphate buffer saline with 0.1% Tween-20 (PBS-T 0.1%) ...
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bioRxiv - Biophysics 2020Quote: ... 40 nM of each labeled Plexin construct was mixed with a serial dilution of unlabeled GTPases or Plexin-B1 in 0.2 mL micro reaction tubes (NanoTemper) and then transferred to premium capillaries (NanoTemper) ...
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bioRxiv - Biophysics 2020Quote: ... and then transferred to premium capillaries (NanoTemper). Microscale thermophoresis measures the change of the diffusion of molecules in microscopic temperature gradients upon protein ligand binding/protein-protein association ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... and Monolith NT.115Pico from NanoTemper Technologies (Munich ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Microbiology 2020Quote: ... The thermal transition midpoint (Tm) and aggregation results from start to finish (Tagg) were reported and analyzed in PR.ThermControl software (NanoTemper Technologies). Three replicates are measured for each sample.
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bioRxiv - Neuroscience 2020Quote: ... The samples were measured in a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA) and MST curves obtained with titration of SNAP25 (1 nM – 125 μM range ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...
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bioRxiv - Biophysics 2020Quote: ... Hydrophobic treated capillaries were filled with the serially diluted samples (Nanotemper). The MST run was performed using a Monolith 1.115 with the LED power at 20% with a measurement time of 20 sec ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper). A 1:1 serial dilution of unlabelled zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2020Quote: ... Affinity Analysis software (NanoTemper Technologies). Figures were prepared using Prism 8 (GraphPad).
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bioRxiv - Biophysics 2020Quote: ... Standard grade nanoDSF capillaries (NanoTemper Technologies) were loaded into a Prometheus NT.48 device (NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: ... ACE2 and spike was monitored by nanoDSF (NanoTemper Technologies) at a concentration of 0.25 mg/ml in PBS (10 mM phosphate ...
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bioRxiv - Biophysics 2020Quote: ... Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies) in PBS-T (10 mM phosphate ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...