Labshake search
Citations for NanoTemper :
851 - 900 of 1145 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... and tested using a Monolith NT.115 instrument (NanoTemper Technologies). Other buffers also include PBS with potassium acetate (Research Products International ...
-
bioRxiv - Molecular Biology 2021Quote: ... Control software (v1.6, NanoTemper Technologies). Measurements were performed in triplicate and data analyses were performed on the MO.Affinity Analysis software (v2.3 ...
-
bioRxiv - Synthetic Biology 2021Quote: Samples were run in triplicate on a Prometheus NT.48 (NanoTemper Technologies) machine set at 100% excitation power ...
-
bioRxiv - Neuroscience 2021Quote: ... using nanoDSF grade standard capillaries (10μL, NanoTemper Technologies). Experiments were set up and monitored with the software PR.ThermControl ...
-
bioRxiv - Molecular Biology 2021Quote: ... The intrinsic fluorescence was measured using the NanoTemper Prometheus NT.48 (NanoTemper, München, Germany).
-
bioRxiv - Biochemistry 2021Quote: ... It was fluorescently labelled with the RED-NHS 2nd Generation kit (NanoTemper Technologies GmbH), and desalted to the reaction buffer (10 mM MES ...
-
bioRxiv - Biochemistry 2021Quote: MST analysis was performed using a Nano Temper Monolith NT.115 instrument (NanoTemper Technologies GmbH). H2A-H2B dimer was reconstituted as before41 ...
-
bioRxiv - Biochemistry 2021Quote: ... g5Rp was first labeled using the Monolith NT Protein Labeling Kit RED-NHS (NanoTemper Technologies), and the labeled protein was then diluted to 20 nM with buffer containing 50 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... The mixtures were loaded into capillaries and measured at ambient temperature by using 20% LED and medium MST power in a Monolith NT.115 system (NanoTemper Technologies). The data were analyzed using NanoTemper analysis software (v.1.2.101).
-
bioRxiv - Biochemistry 2021Quote: The stability of PfGSK3 under different buffer conditions and additives was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The protein concentration was adjusted to 1 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... MST experiments were performed with a Monolith NT.115pico (NanoTemper). A standard protocol was followed 32 ...
-
bioRxiv - Microbiology 2021Quote: ... and loaded into a Prometheus NT.48 device (Nanotemper) controlled by PR.ThermControl (version 2.1.2) ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were loaded into standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
-
bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
-
bioRxiv - Biophysics 2021Quote: The thermal unfolding experiments of the IgLC samples as a function of protein and denaturant concentration were performed with a Prometheus Panta instrument (Nanotemper, Munich, Germany). This is a microcapillary-based (10 μl sample per capillary ...
-
bioRxiv - Biophysics 2021Quote: ... Measurements were carried out in MST-buffer (20 mM phosphate, 120 mM NaCl, at pH 7.4, in 0.005% Tween-20) using premium capillaries (Nanotemper). Proteins were labeled with RED-tris-NHS dye following supplier protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
-
bioRxiv - Cell Biology 2021Quote: DLS measurements were done using the Prometheus Panta (Nanotemper, Munich, Germany). For DLS measurements with good signal-to-noise ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.5°C/min temperature ramp from 20°C to 70°C.
-
bioRxiv - Cell Biology 2021Quote: NanoDSF coupled to scattering measurements were done with the Prometheus NT.48 (Nanotemper, Munich, Germany). Ola1p was diluted to the indicated concentrations in 20 mM PIPES pH 7.0 buffer with a final concentration of 100 mM KCl and 1 mM TCEP ...
-
bioRxiv - Microbiology 2021Quote: ... Quality control during protein purification was carried out using reducing and non-reducing SDS-PAGE analysis and differential scanning fluorimetry (DSF) with a Tycho NT.6 instrument (Nanotemper). All samples were concentrated to 1 mg mL-1 ...
-
bioRxiv - Microbiology 2021Quote: ... smegmatis β clamp (5μM) in the presence and absence of GRS (15μM) were measured in UV-capillaries using the Tycho NT6 (NanoTemper Technologies) where the protein unfolding is followed by detecting the fluorescence of intrinsic tryptophan and tyrosine residues at both emission wavelengths of 350 nm and 330 nm.
-
bioRxiv - Microbiology 2021Quote: ... The results were analyzed in MO.Control and MO.Affinity Analysis software (NanoTemper Technologies GmbH). The results represent an average of at least two independent experiments.
-
bioRxiv - Molecular Biology 2021Quote: ... data of at least two independently pipetted measurements were analysed for the fraction bound (MO.Affinity Analysis software, NanoTemper Technologies). For the non-binders ...
-
bioRxiv - Molecular Biology 2021Quote: ... Data were fitted to the Kd model using MO.Affinity Analysis software (NanoTemper) and were plotted using Prism 8.0.2 (GraphPad).
-
bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: Jagged1C2-EGF3,HA in SEC buffer was labelled with NT-547 dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were transferred to Standard Treated Capillaries (NanoTemper Technologies) and run at 50 % excitation power on a Monolith NT.115 (NanoTemper Technologies ...
-
bioRxiv - Immunology 2021Quote: Thermal stability was determined by differential scanning fluorimetry nano(DSF) on a Prometheus NT.48 instrument (Nanotemper) using first derivative of 350/330nm ratio to determine the melting temperature (Tm ...
-
bioRxiv - Biophysics 2021Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
-
bioRxiv - Neuroscience 2021Quote: ... MST data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies GmbH, Munich, Germany). Statistical significance in cell survival experiments was assessed using one-way ANOVA and unpaired t test with the GraphPad Prism8 software ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.05% Tween-20 in premium coated capillaries (NanoTemper Technologies GmbH; MO-K025) using red LED source ...
-
bioRxiv - Neuroscience 2021Quote: ... Data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies GmbH).
-
bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
bioRxiv - Neuroscience 2021Quote: The microscale thermophoresis (MST) experiments were performed using Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany). Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH ...
-
bioRxiv - Biophysics 2021Quote: ... 115 series premium capillaries (NanoTemper MO-K025). Measurements were performed using 40-60% excitation power and the medium MST intensity option within the default settings in MO.Control software (NanoTemper Technologies ...
-
bioRxiv - Neuroscience 2021Quote: ... buffer at pH 7.5 using nano differential scanning fluorimetry (DSF) instrument Prometheus NT.Plex (NanoTemper Technologies). NanoDSF grade capillaries were filled with 5 µM TAGLN3 solution ...
-
bioRxiv - Neuroscience 2021Quote: ... True label-free fluorescence measurements were performed on a Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies, Munich, Germany) using nanoDSF grade standard capillaries (10μL ...
-
bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
-
bioRxiv - Microbiology 2021Quote: The experiments were performed at 20°C on a Monolith NT.115 (Nanotemper) with Premium Coated (Nanotemper ...
-
bioRxiv - Molecular Biology 2021Quote: ... Signal to noise ratio was used to evaluate the quality of the binding data according to NanoPedia instructions (NanoTemper Technologies). Only a signal-to-noise ratio of more than 5 was considered acceptable while a signal to noise of more than 12 was considered excellent.
-
bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
-
bioRxiv - Molecular Biology 2021Quote: ... experiments were performed according to the NanoTemper technologies protocols in a Monolith NT.115 Pico (Pico Red / Nano Blue - NanoTemper Technologies). His-Cdc42 affinity for the RED-tris-NTA label was not optimal therefore Alexa Fluor 647– NHS dye was used ...
-
bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
-
bioRxiv - Microbiology 2021Quote: ... the samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies). MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies) at an ambient temperature of 25°C ...
-
bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
-
bioRxiv - Microbiology 2021Quote: ... Data of three independently pipetted measurements were analyzed (MO.Affinity Analysis software version 2.3, NanoTemper Technologies).
-
bioRxiv - Plant Biology 2020Quote: MST assays were carried out using a Monolith NT.115 (NanoTemper) machine at the Chemical Genomics Facility of Purdue University ...
-
bioRxiv - Biophysics 2020Quote: ... The final reaction mixtures were loaded into premium capillary chips (Monolith Cat# MO-AK005) and measured on a Monolith NT.115 instrument (NanoTemper Technologies) at 20% excitation power and 40% MST power at 25 °C ...