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Citations for NanoTemper :
1001 - 1050 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: DLS measurements were done using the Prometheus Panta (Nanotemper, Munich, Germany). For DLS measurements with good signal-to-noise ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.5°C/min temperature ramp from 20°C to 70°C.
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bioRxiv - Cell Biology 2021Quote: NanoDSF coupled to scattering measurements were done with the Prometheus NT.48 (Nanotemper, Munich, Germany). Ola1p was diluted to the indicated concentrations in 20 mM PIPES pH 7.0 buffer with a final concentration of 100 mM KCl and 1 mM TCEP ...
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bioRxiv - Microbiology 2021Quote: ... Quality control during protein purification was carried out using reducing and non-reducing SDS-PAGE analysis and differential scanning fluorimetry (DSF) with a Tycho NT.6 instrument (Nanotemper). All samples were concentrated to 1 mg mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... smegmatis β clamp (5μM) in the presence and absence of GRS (15μM) were measured in UV-capillaries using the Tycho NT6 (NanoTemper Technologies) where the protein unfolding is followed by detecting the fluorescence of intrinsic tryptophan and tyrosine residues at both emission wavelengths of 350 nm and 330 nm.
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bioRxiv - Microbiology 2021Quote: ... The results were analyzed in MO.Control and MO.Affinity Analysis software (NanoTemper Technologies GmbH). The results represent an average of at least two independent experiments.
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bioRxiv - Molecular Biology 2021Quote: ... data of at least two independently pipetted measurements were analysed for the fraction bound (MO.Affinity Analysis software, NanoTemper Technologies). For the non-binders ...
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bioRxiv - Molecular Biology 2021Quote: ... Data were fitted to the Kd model using MO.Affinity Analysis software (NanoTemper) and were plotted using Prism 8.0.2 (GraphPad).
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Jagged1C2-EGF3,HA in SEC buffer was labelled with NT-547 dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were transferred to Standard Treated Capillaries (NanoTemper Technologies) and run at 50 % excitation power on a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Immunology 2021Quote: Thermal stability was determined by differential scanning fluorimetry nano(DSF) on a Prometheus NT.48 instrument (Nanotemper) using first derivative of 350/330nm ratio to determine the melting temperature (Tm ...
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bioRxiv - Biophysics 2021Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
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bioRxiv - Neuroscience 2021Quote: ... MST data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies GmbH, Munich, Germany). Statistical significance in cell survival experiments was assessed using one-way ANOVA and unpaired t test with the GraphPad Prism8 software ...
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bioRxiv - Neuroscience 2021Quote: ... 0.05% Tween-20 in premium coated capillaries (NanoTemper Technologies GmbH; MO-K025) using red LED source ...
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bioRxiv - Neuroscience 2021Quote: ... Data analysis was performed using MO.Affinity Analysis v2.3 software (NanoTemper Technologies GmbH).
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Neuroscience 2021Quote: The microscale thermophoresis (MST) experiments were performed using Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany). Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH ...
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bioRxiv - Biophysics 2021Quote: ... 115 series premium capillaries (NanoTemper MO-K025). Measurements were performed using 40-60% excitation power and the medium MST intensity option within the default settings in MO.Control software (NanoTemper Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... buffer at pH 7.5 using nano differential scanning fluorimetry (DSF) instrument Prometheus NT.Plex (NanoTemper Technologies). NanoDSF grade capillaries were filled with 5 µM TAGLN3 solution ...
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bioRxiv - Neuroscience 2021Quote: ... True label-free fluorescence measurements were performed on a Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies, Munich, Germany) using nanoDSF grade standard capillaries (10μL ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
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bioRxiv - Microbiology 2021Quote: The experiments were performed at 20°C on a Monolith NT.115 (Nanotemper) with Premium Coated (Nanotemper ...
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bioRxiv - Molecular Biology 2021Quote: ... Signal to noise ratio was used to evaluate the quality of the binding data according to NanoPedia instructions (NanoTemper Technologies). Only a signal-to-noise ratio of more than 5 was considered acceptable while a signal to noise of more than 12 was considered excellent.
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed according to the NanoTemper technologies protocols in a Monolith NT.115 Pico (Pico Red / Nano Blue - NanoTemper Technologies). His-Cdc42 affinity for the RED-tris-NTA label was not optimal therefore Alexa Fluor 647– NHS dye was used ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Microbiology 2021Quote: ... the samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies). MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Microbiology 2021Quote: ... MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies) at an ambient temperature of 25°C ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Data of three independently pipetted measurements were analyzed (MO.Affinity Analysis software version 2.3, NanoTemper Technologies).
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bioRxiv - Plant Biology 2020Quote: MST assays were carried out using a Monolith NT.115 (NanoTemper) machine at the Chemical Genomics Facility of Purdue University ...
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bioRxiv - Biophysics 2020Quote: ... The final reaction mixtures were loaded into premium capillary chips (Monolith Cat# MO-AK005) and measured on a Monolith NT.115 instrument (NanoTemper Technologies) at 20% excitation power and 40% MST power at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded into Monolith NT.115 Capillaries and ran on a Monolith NT.115 (NanoTemper, München, Germany). All experiments were run at 25°C with 40% laser excitation ...
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bioRxiv - Cell Biology 2020Quote: ... his-tagged TLNRD1 was diluted to 100 nM and coupled with His-tag NT-647 dye (RED-tris-NTA NanoTemper, München, Germany) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cell Biology 2020Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (Nanotemper Technologies). A red LED at 10% excitation power (red filter ...
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bioRxiv - Cell Biology 2020Quote: ... Data analysis was performed with NTAffinityAnalysis software (NanoTemper Technologies) in which the binding isotherms were derived from the raw fluorescence data and then fitted with both NanoTemper software and GraphPad Prism to determine the KD using a non-linear regression method ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The measurements were done in premium coated capillaries (NanoTemper Technologies GmbH, MO-K025) using red LED source ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: The experiments have been performed using Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany). Recombinant proteins were labeled through His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (MO-L008) ...
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bioRxiv - Molecular Biology 2020Quote: ... The results were analyzed using the MO Affinity Analysis software (NanoTemper Technologies). The dissociation constant (KD ...
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bioRxiv - Molecular Biology 2020Quote: ... the measurements were performed on a Monolith NT.115 system (NanoTemper Technologies). We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Intrinsic protein fluorescence at 330 nm and 350 nm was monitored between 15 and 90 °C in the Prometheus NT.48 instrument (Nanotemper), and the Tm values calculated within the accompanying software by taking the turning point of the first derivative of the F350:F330 ratio as a function of temperature.
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bioRxiv - Molecular Biology 2020Quote: ... were loaded into glass capillaries (Nanotemper) by capillary action ...
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bioRxiv - Molecular Biology 2020Quote: ... The aggregation temperature for each experiment was also automatically calculated by Nanotemper PR ...
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bioRxiv - Molecular Biology 2020Quote: ... The melting temperature for each experiment was automatically calculated by Nanotemper PR ...
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bioRxiv - Molecular Biology 2020Quote: Differential scanning fluorimetry experiments were performed using the Prometheus NT.48 instrument (NanoTemper Technologies). NanR was prepared at 30 μM in buffer C ...
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bioRxiv - Molecular Biology 2020Quote: ... 10-µL of undiluted rAAV of each lot was loaded into a high sensitivity capillary (Nanotemper Cat#PR-C006) and run on a Nanotemper Prometheus NT.48 nanoDSF instrument ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with Neu5Ac (20 mM) Data analysis was performed using PR.ThermControl software (NanoTemper Technologies) where an apparent melting point (Tm ...