Labshake search
Citations for NanoTemper :
751 - 800 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... All reactions were incubated for 20 minutes at 25 ºC before loading into premium glass capillaries (NanoTemper Technologies). Microscale thermophoresis (MST ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were loaded to the capillaries (NanoTemper Technologies, catalog #: MO-K025) and measured using a Monolith NT.115 instrument with the settings of 40% LED/excitation and 40% MST power ...
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bioRxiv - Cell Biology 2022Quote: ... Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies, catalog #: MO-L018) was used to label recombinant His-α1-ERD (MyBioSource ...
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bioRxiv - Cell Biology 2022Quote: ... using a Monolith NT.115 instrument (NanoTemper Technologies Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... was mixed with 16 serial dilutions of strep-NHR-85 starting at 31.5 uM and loaded into microscale thermophoresis premium coated capillaries (NanoTemper Technologies). MST measurements were recorded at 25°C using 30% excitation power and 60% MST power ...
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bioRxiv - Developmental Biology 2022Quote: Binding assays of purified NHR23 or strep-NHR85 was measured using a Monolith NT.115 Pico running MO Control version 1.6 (NanoTemper Technologies). Assays were performed in 100 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... and to liposomes was assessed by MST using a Monolith NT.115 system (Nanotemper Technologies, Munich, Germany). Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were performed in triplicates and data were fitted using the KD model of the MO.Affinity Analysis software (Nanotemper Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... Around 12 μL of protein complex at a concentration of 0.15 mg/mL in buffer B were applied into standard grade NanoDSF glass capillaries (Nanotemper). Capillaries were loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Molecular Biology 2022Quote: ... Capillaries were loaded into a Prometheus NT.48 device (Nanotemper) and excitation power was set to 80% using the PR.ThermControl v.2.3.1 software ...
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bioRxiv - Molecular Biology 2022Quote: ... Data of three independently pipetted measurements were analysed (MO.Affinity Analysis software version 2.3, NanoTemper Technologies) using the signal from an MST-on time of 2.5s.
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bioRxiv - Molecular Biology 2022Quote: ... MST was measured using a Monolith NT.115 [NT.115Pico/N.T.LabelFree] instrument (NanoTemper Technologies) at an ambient temperature of 22 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and loaded into Monolith NT.115 [Premium] Capillaries (NanoTemper Technologies). MST was measured using a Monolith NT.115 [NT.115Pico/N.T.LabelFree] instrument (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Microscale thermophoresis (MST) experiments were performed on a Monolith NT.115 Pico instrument (Nanotemper Technologies, München, Germany) to measure the binding affinity between AAT and the GR ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Data were analyzed using the MO Affinity Analysis software suite (Nanotemper Technologies, Munich, Germany).
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bioRxiv - Microbiology 2022Quote: MST experiments were carried out at 22 °C on Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany) as described in the review entitled Molecular interaction studies using microscale thermophoresis 37 ...
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bioRxiv - Microbiology 2022Quote: NanoDSF was performed using Prometheus NT.48 equipped with back reflection mode (NanoTemper Technologies, München, Germany). XccXopP ...
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bioRxiv - Microbiology 2022Quote: ... were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd generation according to the recommendations of the manufacturer (NanoTemper Technologies). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: The thermal stability of the proteins was determined using Prometheus NT.48 nanoDSF instrument from NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Raw data were processed using NT Analysis software (NanoTemper Technologies) and fit to a Hill binding model to determine the EC50 and Hill coefficient.
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bioRxiv - Molecular Biology 2022Quote: ... Thermophoresis measurements were collected at 25°C using a Monolith NT.115 (NanoTemper Technologies) set to medium LED power and 20% excitation power ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Binding assays were performed using NanoTemper NT.LabelFree instrument (NanoTemper Technologies). All experiments were carried out at room temperature in hydrophobic capillaries with 20% light-emitting diode power (fluorescence lamp intensity ...
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bioRxiv - Plant Biology 2022Quote: ... and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH, Munich, Germany) set to an LED power of 60% to 80% ...
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bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
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bioRxiv - Plant Biology 2022Quote: Protein sample preparation and measurements were performed according to the protocol from Monolith NT (NanoTemper Technologies GmbH, Munich, Germany), employing quality controls as outlined by Sedivy 202143 ...
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bioRxiv - Plant Biology 2022Quote: ... Calculation of melting temperature inflection points was performed using PR.Therm Control software (NanoTemper GmbH, Munich, Germany).
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bioRxiv - Plant Biology 2022Quote: ... MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany) at ambient temperature of 23 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Plant Biology 2022Quote: ... Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Microbiology 2022Quote: ... and the measurements were performed using a Monolith Pico instrument (NanoTemper Technologies) under the optimized experimental condition (50% excitation power ...
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bioRxiv - Microbiology 2022Quote: ... The mixed samples were then loaded into the Monolith NT.115 capillaries (NanoTemper Technologies) and the measurements were performed using a Monolith Pico instrument (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Automated LabelFree Premium Capillary Chips (NanoTemper Technologies) and the measurements were started ...
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bioRxiv - Molecular Biology 2022Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
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bioRxiv - Microbiology 2022Quote: ... Normalization of the fluorescence signal and fitting to the Hill equation were performed using the software MO Affinity Analysis v2.3 (NanoTemper Technologies). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and analyzed with a NanoTemper Monolith NT.115 (NanoTemper Technologies) at 25 °C ...
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bioRxiv - Molecular Biology 2022Quote: All microscale thermophoresis measurements (MST) were performed on a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). using the Standard Treated Capillaries K002 of the supplier ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The data was analyzed using the MO.affinity analysis software (V2.3, NanoTemper Technologies) and binding reactions were determined by analysing changes in Temperature Related Intensity Changes (TRIC-effect) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20% LED power in standard capillaries at 25°C on a Monolith NT.115 device (NanoTemper Technologies). The data was analyzed using the MO.affinity analysis software (V2.3 ...
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bioRxiv - Molecular Biology 2022Quote: The kinetic measurements of PfCoSP-gDNA binding were conducted using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Tycho analysis (Tycho NT.6, NanoTemper Technologies GmbH, Munich, Germany) to follow the interaction between VcDnaB or EcDnaB and VcDciA ...
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bioRxiv - Molecular Biology 2022Quote: Binding assays were performed by electrophoretic mobility shift assay (EMSA) and Microscale thermophoresis (MST, NanoTemper Munich). Dilution series (1.5 fold ...
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bioRxiv - Microbiology 2022Quote: ... AtExo70B1 and their complex were loaded in nanoDSF grade standard capillaries (NanoTemper Technologies GmbH, München, Germany) and exposed at thermal stress from 20 ⍰C to 95 ⍰C by thermal ramping rate of 1 aC/min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies with an excitation wavelength of 280 nm ...
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bioRxiv - Plant Biology 2022Quote: ... the Kd (the dissociation constant) values between the effector and the RGA5 HMAs were measured using Monolith NT.115 (NanoTemper Technologies) with 30 % LED power and fitted with Nano Temper Analysis Software (Version 1.5.41) ...