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Citations for NanoTemper :
1051 - 1100 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The aggregation temperature for each experiment was also calculated automatically by Nanotemper PR ...
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bioRxiv - Molecular Biology 2020Quote: ... The melting temperature for each experiment was calculated automatically by Nanotemper PR ...
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bioRxiv - Molecular Biology 2020Quote: ... 115 (NanoTemper Technologies GmbH, Munich, Germany). RNP complex (Protein:sgRNA molar ratio,1:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... where sample was loaded into standard treated capillaries provided by Nanotemper. Measurements were performed using 60% excitation power in blue filter (465-490nm excitation wavelength ...
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bioRxiv - Molecular Biology 2020Quote: ... 115 (NanoTemper Technologies GmbH, Munich, Germany) where sample was loaded into standard treated capillaries provided by Nanotemper ...
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bioRxiv - Molecular Biology 2020Quote: ... Automated (NanoTemper, Germany). KD determination was performed using the MO.Affinity Analysis (NanoTemper ...
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bioRxiv - Biophysics 2020Quote: ... were loaded into a Prometheus NT.48 device (Nanotemper) controlled by PR ...
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bioRxiv - Biophysics 2020Quote: ... Standard grade nanoDSF capillaries (Nanotemper) were loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Developmental Biology 2020Quote: ... Affinity Analysis software version 2.1 (NanoTemper Technologies). For fluorescence normalization (Fnorm = Fhot/Fcold) ...
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bioRxiv - Developmental Biology 2020Quote: ... and placed into the Monolith NT.115 instrument (NanoTemper Technologies). Experiments were conducted with 80% LED and 80% MST power ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were then aspirated into NT.115 Standard Treated Capillaries (NanoTemper Technologies) and placed into the Monolith NT.115 instrument (NanoTemper Technologies) ...
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bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... The normalized change in fluorescence was plotted as a function of DNA concentration and analyzed using the MO.Affinity Analysis software (NanoTemper Technologies). Oligonucleotides sequences are listed in Supplementary Table 3.
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bioRxiv - Plant Biology 2020Quote: ... and the thermophoretic response was monitored with 30 % infrared (IR) laser intensity and 80 % MST power using a Monolith NT.115 instrument (NanoTemper Technologies) at 20 °C ...
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bioRxiv - Plant Biology 2020Quote: DNA binding reactions were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and the thermophoretic response was monitored with 30 % infrared (IR ...
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bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
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bioRxiv - Biochemistry 2020Quote: ... using a Tycho NT.6 instrument (Nanotemper), which allowed for determination of NT-mini’s unfolding temperatures in the different assayed conditions ...
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bioRxiv - Biophysics 2020Quote: ... Samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies), and thermophoresis and raw fluorescence signals measured at 25 °C with a light-emitting diode (LED ...
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bioRxiv - Biophysics 2020Quote: hGH was labelled with NT-647-NHS88 using the Monolith NT™ Protein Labelling Kit RED-NHS (NanoTemper Technologies) for 1 h at room temperature with NT-647-NHS at a molar ratio of 1:3 in labelling buffer following the protocol ...
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bioRxiv - Biophysics 2020Quote: ... The equilibrium binding between 20 nM NT-647-NHS labelled hGH to hGHR(MSP1D1) was calculated from the change in thermophoresis ΔFnorm = ΔFhot/ΔFcold measured on a Monolith NT.115 (NanoTemper Technologies). For hGHG120R the raw fluorescence change was used to determine the binding affinity ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analyses and the dissociation constants (Kd) calculation were performed using MO Affinity Analysis software (NanoTemper Technologies, Munich, Germany). Binding of radiolabeled GTP was measured by means of the SPA ...
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bioRxiv - Cancer Biology 2020Quote: MicroScale Thermophoresis experiments were performed according to the NanoTemper Technologies protocol in a Monolith NT.115 (red) instrument (NanoTemper Technologies, Munich, Germany). Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH ...
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Biochemistry 2020Quote: The MST experiments were performed using a Monolith NT.115 instrument (NanoTemper). Fluorescin-5-Maleimide labeled RcdA was incubated with increasing concentrations of unlabeled RcdA in the same buffer used in the polarization experiments ...
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bioRxiv - Biochemistry 2020Quote: ... The Kd values were calculated using MO Affinity Analysis software (NanoTemper) and fit to the nonlinear equation Fnorm = [unbound + (bound-unbound ...
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bioRxiv - Biochemistry 2020Quote: ... was applied to a dilution series of purified His6-GlnA1 ranging from nM to µM concentrations and measured using standard treated capillaries (NanoTemper, Munich, Germany), 100% Excitation Power and medium MST-Power.
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bioRxiv - Biochemistry 2020Quote: ... Data of three independent pipetted measurements were analyzed (MO.Affinity Analysis software version 2.3, NanoTemper Technology).
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnA1 was analyzed using Tycho NT.6 (NanoTemper, Munich, Germany) by applying a standard capillary (10 µl ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnK1 was purified and labeled using Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) (DOL ...
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bioRxiv - Biochemistry 2020Quote: Purified His6-GlnA1 was fluorescently labeled using the Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) with buffer B (50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Data were analyzed using the NT Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2020Quote: ... A Monolith NT.115 instrument (NanoTemper Technologies) was used with a 50% LED power and laser pulses at both 60% and 100% power ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The thermophoresis signal was then measured on a Monolith NT.115 device (Nanotemper) with premium capillaries (MO-K025) ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Biochemistry 2020Quote: Using the Prometheus NT.48 instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... 2.3 (NanoTemper Technologies).
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bioRxiv - Biochemistry 2020Quote: The microscale thermophoresis (MST) assay was performed using a Monolith NT.115 instrument (NanoTemper Technologies). For the MST assay ...
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Biochemistry 2020Quote: ... and measured in duplicates in a Prometheus NT.48 (NanoTemper) with the following settings ...
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bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... Microscale thermophoresis assays were performed on a NanoTemper ® Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany). Samples were prepared in 20mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biochemistry 2020Quote: Microscale thermophoresis was performed using a Monolith NT.115 from Nanotemper Technologies (Munich ...
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bioRxiv - Biochemistry 2020Quote: ... Experiments were performed in triplicates and data were fitted using the Kd model with the MO.Affinity Analysis software (Nanotemper Technologies, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Biochemistry 2020Quote: ... the SpaKC sample was loaded to column B (Nanotemper MO-L011) and eluted with 450 μL of assay buffer (50mM Na2CO3/NaHCO3 ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were then loaded into Monolith NT.115 capillary (Nanotemper Technologies) and measured by using 20% (Auto-detect ...