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Citations for NanoTemper :
1101 - 1150 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the binding affinity between SpaKC and SpaAleader was measured using Monolith NT.115 Pico (Nanotemper Technologies). SpaKC was fluorescently labelled by the labeling kit (Monolith Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive) ...
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bioRxiv - Plant Biology 2020Quote: ... The intrinsic fluorescence signal was measured as a function of increasing temperature with a Prometheus NT.48 fluorimeter (NanoTemper Technologies), with 55% excitation light intensity and 1 °C/min temperature ramp ...
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: The binding of W25 and the structural integrity of Spike RBD was verified using a label-free thermal shift assay with Tycho NT.6 (Nanotemper Technologies GmbH) using intrinsic tryptophan and tyrosine fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... W25 was fluorescently labeled with the Monolith Protein Labeling Kit RED – NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH) following the protocol established in the manual ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity measurements were conducted in Premium Capillaries Monolith NT.115 (MO-K025, NanoTemper Technologies GmbH) and repeated three times ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity Analysis v.2.3 (NanoTemper Technologies, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... and the Monolith NT.115 Premium Capillaries (NanoTemper Technologies, Germany).
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bioRxiv - Molecular Biology 2020Quote: The MST experiments were performed in a Monolith NT.115 Pico (NanoTemper Technologies, Germany) using the Monolith Protein Labeling Kit RED-NHS (Amine Reactive) ...
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bioRxiv - Microbiology 2020Quote: ... NanoDSF profiles were measured with a Tycho system (Nanotemper). The first derivative profiles were then fit as a sum of Gaussian curves in Excel (Microsoft).
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bioRxiv - Biophysics 2020Quote: ... Measurements at 80°C required capillary sealing (Capillary Sealing Paste and Capillary Sealing Applicators; NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
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Identification of glutamine synthetase as a novel contryphan-Bt binding protein by his-tag pull downbioRxiv - Pharmacology and Toxicology 2020Quote: Microscale thermophoresis (MST) experiments were conducted on a NanoTemper Monolith NT.115 system (NanoTemper, Germany). Recombinant human glutamine synthetase (GS ...
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bioRxiv - Biophysics 2020Quote: ... 115 Pico (NanoTemper Technologies) at 25°C ...
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bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biophysics 2020Quote: Binding experiments were performed using a Monolith NT.115 Microscale Thermophoresis (MST) instrument (NanoTemper) at room temperature in phosphate buffer saline with 0.1% Tween-20 (PBS-T 0.1%) ...
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bioRxiv - Biophysics 2020Quote: ... 40 nM of each labeled Plexin construct was mixed with a serial dilution of unlabeled GTPases or Plexin-B1 in 0.2 mL micro reaction tubes (NanoTemper) and then transferred to premium capillaries (NanoTemper) ...
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bioRxiv - Biophysics 2020Quote: ... and then transferred to premium capillaries (NanoTemper). Microscale thermophoresis measures the change of the diffusion of molecules in microscopic temperature gradients upon protein ligand binding/protein-protein association ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... and Monolith NT.115Pico from NanoTemper Technologies (Munich ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Microbiology 2020Quote: ... The thermal transition midpoint (Tm) and aggregation results from start to finish (Tagg) were reported and analyzed in PR.ThermControl software (NanoTemper Technologies). Three replicates are measured for each sample.
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bioRxiv - Neuroscience 2020Quote: ... The samples were measured in a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA) and MST curves obtained with titration of SNAP25 (1 nM – 125 μM range ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...
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bioRxiv - Biophysics 2020Quote: ... Hydrophobic treated capillaries were filled with the serially diluted samples (Nanotemper). The MST run was performed using a Monolith 1.115 with the LED power at 20% with a measurement time of 20 sec ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper). A 1:1 serial dilution of unlabelled zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2020Quote: ... Affinity Analysis software (NanoTemper Technologies). Figures were prepared using Prism 8 (GraphPad).
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bioRxiv - Biophysics 2020Quote: ... Standard grade nanoDSF capillaries (NanoTemper Technologies) were loaded into a Prometheus NT.48 device (NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: ... ACE2 and spike was monitored by nanoDSF (NanoTemper Technologies) at a concentration of 0.25 mg/ml in PBS (10 mM phosphate ...
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bioRxiv - Biophysics 2020Quote: ... Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies) in PBS-T (10 mM phosphate ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: ... were loaded into a Prometheus NT.48 device (NanoTemper Technologies) controlled by PR ...
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bioRxiv - Microbiology 2020Quote: ... The samples were loaded into standard treated capillaries (Nanotemper) using 70% LED and 80% IR-laser power ...
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bioRxiv - Microbiology 2020Quote: MST experiments were performed on a Monolith NT.115 system (Nanotemper Technologies, San Francisco, CA, USA). A solution of unlabeled formaldehyde was serially diluted in reaction buffer (10 mM Na2HPO4 ...
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bioRxiv - Immunology 2020Quote: Measurements were performed on a Prometheus NT.48 NanoDSF instrument (NanoTemper Technologies) as described previously (72) ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... TmrAB was diluted to 8.5 μM in SEC buffer and prepared in duplicate for measurement on a Tycho NT.6 (NanoTemper Technologies). Data are reported as the ratio of fluorescence intensity at 350 nm and 330 nm normalized to the minimum and maximum values of the whole dataset.
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bioRxiv - Biochemistry 2020Quote: ... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 10 μL of each diluted sample was loaded into nanoDSF grade high-sensitivity capillaries (NanoTemper) in triplicate and placed on the instrument’s capillary loading tray ...
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bioRxiv - Biochemistry 2020Quote: ... Experiments were performed using the Prometheus NT.48 nanoDSF (NanoTemper Technologies, Germany). For this work ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The data were acquired with MO.Control 1.6.1 (NanoTemper Technologies). Recorded data were analyzed with MO.Affinity Analysis 2.3 (NanoTemper Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Experiments were run at 20% excitation and high MST power at 23.0°C on a Monolith NT.115Pico (NanoTemper). Each experimental compound was run in triplicate.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recorded data were analyzed with MO.Affinity Analysis 2.3 (NanoTemper Technologies). The dissociation constant Kd quantifies the equilibrium of the reaction of the labelled molecule A (concentration cA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Experiments were performed using a Monolith Pico (Nanotemper). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermal stability of Conk-C3 derivatives was determined by differential scanning fluorimetry on a NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany) with a back-reflection aggregation detection at a range from 35 to 95°C and with a heating rate of 20°C min-1 ...
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bioRxiv - Biochemistry 2020Quote: Protein melting temperatures were determined by difference scanning fluorimetry utilizing the intrinsic fluorescence of tryptophan and tyrosine measured in a Tycho NT6 System (NanoTemper, Munich, Germany). Results are average of three independent measurements with two technical replicates for each measurement.
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bioRxiv - Molecular Biology 2020Quote: ... PrgU-His was labeled with the RED 2nd generation His-tag labeling kit (NanoTemper Technologies) according to the manufacturer’s instructions with a final concentration in the assay of 50 nM (molar dye ...