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Citations for NanoTemper :
1101 - 1145 of 1145 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The data were analyzed using MO Affinity Analysis software (Nanotemper GmbH).
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bioRxiv - Biochemistry 2019Quote: ... Monolith NT 1.115 Premium Capillaries (NanoTemper) were filled with each reaction mixture ...
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bioRxiv - Biochemistry 2019Quote: ... loaded on a cartridge into the Monolith NT 1.115 (NanoTemper) and MST analysis was performed using the following parameters ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and the samples were loaded into Monolith NT.115 Capillaries (NanoTemper Technologies). Instrument parameters were adjusted to 80% LED power and medium MST power (40%) ...
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bioRxiv - Immunology 2019Quote: ... Data of three independently pipetted measurements were analyzed (MO.Affinity Analysis software version 2.3, NanoTemper Technologies) using the signal from an MST-on time of 20s.
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bioRxiv - Molecular Biology 2019Quote: ... 115 (NanoTemper Technologies GmbH, Munich, Germany). RNP complex (Protein:sgRNA molar ratio ...
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bioRxiv - Bioengineering 2019Quote: Dissociation constants were measured using Microscale Thermophoresis (MST) on a Monolith NT.115 instrument (NanoTemper, Germany). Thermal migrations were monitored in real time by using a FAM-labeled probe (probeFAM ...
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bioRxiv - Bioengineering 2019Quote: ... The reactions were loaded into MO-K022 standard capillaries (NanoTemper, Germany) and measured ...
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bioRxiv - Biophysics 2019Quote: Thermal protein denaturation stability analysis was performed via differential scanning fluorimetry (using intrinsic fluorescence of tryptophan residues) by slowly heating the sample in a Prometheus NT.48 scanning fluorimeter (NanoTemper Technologies, GmbH). Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Microbiology 2019Quote: ... CqsR-LBD was diluted to 20 μM and labeled using the Monolith NT Protein Labeling Ki RED-NHS (NanoTemper Technologies). Different concentrations of ethanolamine were incubated with 20 nM working stock solutions of labeled protein in the dark for 30 min at 4° C ...
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bioRxiv - Microbiology 2019Quote: ... the samples were transferred into standard treated capillaries (NanoTemper Technologies) and read in a Monolith NT.115 Blue/Red instrument at room temperature using 20% LED and medium MST power ...
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bioRxiv - Biochemistry 2019Quote: Microscale thermophoresis was carried out in standard capillaries (Cat# MO-K022, Nanotemper Technologies, Munich, Germany) on a Monolith NT.115Pico instrument (Nanotemper Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... on a Monolith NT.115Pico instrument (Nanotemper Technologies) using the Pico-RED detector with 30% LED power at 25 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Data were analyzed with MO.Affinity Analysis 2.2.7 software (NanoTemper Technologies). The integral of thermophoresis traces between 1 s to 20 s on-time was used for EC50 determination and the normalized flourescence difference ΔFnorm was plotted against ligand concentration for dose-response plots ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were loaded into nanoDSF Grade Standard capillaries (Cat# PR-C002, NanoTemper Technologies) in duplicates and transferred to a Prometheus NT.48 instrument (NanoTemper Technologies) ...
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bioRxiv - Biochemistry 2019Quote: ... in duplicates and transferred to a Prometheus NT.48 instrument (NanoTemper Technologies). Thermal unfolding was detected by recording the intrinsic tryptophan fluorescence (emission ratio at 350 nm and 330 nm ...
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bioRxiv - Microbiology 2019Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Measurements were performed in standard capillaries with a Monolith NT.115 Pico system at 20% excitation power and 40% MST power (NanoTemper Technologies).
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bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...
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bioRxiv - Microbiology 2019Quote: MicroScale Thermophoresis (MST) experiments were performed using the Monolith NT.115 system (NanoTemper Technologies, Germany). The laser power was set at 20% and the LED at 100% ...
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bioRxiv - Microbiology 2019Quote: ... Equilibrated mixtures containing 5µM of PdtaS-EGFP and varying concentrations of c-di-GMP were loading onto hydrophobic capillaries (NanoTemper Technologies). The laser on and off times were set at 30s and 5s respectively ...
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bioRxiv - Microbiology 2019Quote: ... the Kd values were determined using NanoTemper Analysis software (NanoTemper Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The mixtures were incubated for 15 min and added into glass capillaries and loaded into a Monolith NT.115 instrument (NanoTemper Technologies, Germany), the Kd values were determined using NanoTemper Analysis software (NanoTemper Technologies ...
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bioRxiv - Microbiology 2019Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
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bioRxiv - Microbiology 2019Quote: ... The MST data were analyzed using the NanoTemper analysis software (NanoTemper Technologies GmbH) and plotted using GraphPad Prism 6.0 software ...
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bioRxiv - Biochemistry 2019Quote: Microscale thermophoresis (MST) measurements were performed using a NanoTempervMonolithTM NT.115 instrument (NanoTemper Technologies GmbH, München, Germany). Protein samples were labelled with the amine reactive dye NT-647 using the Monolith™ NT.115 Protein Labeling Kit RED-NHS ...
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bioRxiv - Biochemistry 2019Quote: ... was labelled with 100 μM Monolith NTTM His-Tag Labeling kit RED-tris-NTA (L008, NANOTEMPER, Munich, Germany) 30 min at room temperature (RT ...
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bioRxiv - Biochemistry 2019Quote: Measurements were performed using the Monolith NT.115 MST Premium Coated Capillaries (K005, NANOTEMPER, Munich, Germany), excitation Power 20% ...
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bioRxiv - Biochemistry 2019Quote: ... with the Monolith NT.115 Pico instrument (NANOTEMPER, Munich, Germany).
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bioRxiv - Biochemistry 2019Quote: Apparent Kd values were computed fitting the compound concentration-dependent changes in normalized fluorescence (Fnorm) by the MO Affinity Analysis software provided by Nanotemper. Final results were obtained averaging four independent experiments.
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... MST analysis was performed by loading 4µl of the supernatant into premium glass capillaries (NanoTemper Technologies) at MST power of 40% and LED power of 80% ...
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bioRxiv - Cancer Biology 2019Quote: ... at 22°C temperature using a Monolith NT.115 (NanoTemper Technologies). An initial “Capillary Scan” was performed to scan for fluorescence across the capillary tray to determine the exact position of each capillary before the MST measurement was started ...
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bioRxiv - Microbiology 2019Quote: The microscale thermophoresis experiments were conducted by Monolith NT0.115 instrument (NanoTemper Technologies) at 40% MST and LED power at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The binding affinities were determined to fit the data points to a quadratic equation (equation 1) using non-linear regression using MO Affinity software (NanoTemper Technologies), Prism (GraphPad Inc ...
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bioRxiv - Biochemistry 2019Quote: MST was measured on 10 μl reactions using standard capillaries (MO-K022, NanoTemper Technologies) on a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... on a Monolith NT.115 (NanoTemper Technologies) at monochromatic LED with power input of 30% and IR laser power of 40% ...
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bioRxiv - Biochemistry 2019Quote: ... KD were calculated using a hyperbolic or quadratic function using Graphpad Prism 6.0 (GraphPad Inc, USA) or software provided by NanoTemper. Same-site inhibitory constants were calculated using the KD values obtained for GTP and concentrations used by non-linear fitting using the one site competition function using Graphpad Prism 6.0 (GraphPad Inc ...
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bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded into MST Premium Coated Capillaries (Nanotemper) and analyzed using 20% blue LED power for fluorescence excitation and Medium infrared laser power for the thermophoresis ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded in the standard treated capillaries and measured in a NanoTemper Monolith NT.115 LabelFree instrument (NanoTemper Technologies, München, Germany). The samples were measured at LED power of 40% ...