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Citations for NanoTemper :
601 - 650 of 1068 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Then the mixture was incubated overnight at 16 °C followed by centrifugation at 15,000 g for 15 min before loaded into the Premium Capillaries (NanoTemper Technologies). The thermophoresis analysis ...
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bioRxiv - Molecular Biology 2022Quote: Thermal stability was measured using the Prometheus NT.48 nano-DSF instrument (NanoTemper). LRRK1 or the relevant mutant in the buffer 20 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
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bioRxiv - Biochemistry 2022Quote: ... The Kd value was calculated using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2022Quote: The affinity of SLC19A1 for TPP was determined using a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies). The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies) in Buffer D (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Affinity Analysis Software v2.3 (NanoTemper Technologies) was used to analyze the data and get the KD value of SAC.
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bioRxiv - Molecular Biology 2022Quote: ... Automated instrument (NanoTemper Technologies) via the label-free method ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... Standard capillaries were filled with the samples for measurements with NanoTemper Monolith NT.115 instrument (NanoTemper, Germany). The measurement was performed in standard capillaries (NanoTemper ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The measurement was performed in standard capillaries (NanoTemper, MO-Z022, Germany) at 20% LED and 50% MST power with laser-On time 30 sec and laser-off time 5 s.
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bioRxiv - Biochemistry 2022Quote: ... Data were acquired with a Tycho NT.6 equipment (NanoTemper Technologies). The emission fluorescence intensity was recorded at 330 nm and 35 ºC ...
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bioRxiv - Immunology 2022Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Biochemistry 2022Quote: MST experiments were performed using a Monolith NT.115 instrument (NanoTemper Technologies, Germany). In all cases premium treated capillaries were used and the experiment conducted at 25°C ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM NaCl and transferred to capillaries (Standard treated, NanoTemper Technologies GmbH, Germany), measurements were performed at 37 °C with a constant blue LED power of 20% and at MST infrared laser powers of 40 % to induce thermophoretic motion ...
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bioRxiv - Biophysics 2021Quote: The differential scanning fluorimetry (DSF) method was used to follow the thermal unfolding event of HsPepT2 with a Prometheus NT.48 device (NanoTemper Technologies, Munich, Germany). Purified HsPepT2 was diluted to 0.3 mg/mL and supplemented with decreasing amounts of Ala-Phe dipeptide in a dilution series of 13 points starting at 10 mM down to 2.4 µM ...
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bioRxiv - Biophysics 2021Quote: ... Measurements were recorded on a Monolith NT automated instrument (NanoTemper Technologies GmbH, Germany) with a red-laser channel at 5% LED excitation power and medium MST power at ambient conditions ...
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bioRxiv - Biophysics 2021Quote: Analysis of MST traces was completed using MO.Affinity Analysis v2.3 (Nanotemper) (30) ...
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bioRxiv - Microbiology 2021Quote: ... The labelling of NyxA was performed by covalent coupling of the lysines of the protein to a fluorophore using the RED-NHS (Nanotemper) Protein Labeling Kit ...
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bioRxiv - Microbiology 2021Quote: ... with Premium Coated (Nanotemper) capillaries ...
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bioRxiv - Genetics 2021Quote: ... Control software (NanoTemper Technologies). M.O ...
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bioRxiv - Genetics 2021Quote: ... They were then loaded into standard capillaries (NanoTemper Technologies). Reactions were then tested with 40% excitation power ...
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bioRxiv - Genetics 2021Quote: MST was performed using a Monolith NT.115 instrument (NanoTemper Technologies) equipped with red and blue filters using methods described in Hosford et al ...
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bioRxiv - Genetics 2021Quote: ... Affinity Analysis software (version 2.3, Nanotemper Technologies) was used to analyze data and determine the normalized fluorescence (Fnom ...
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bioRxiv - Microbiology 2021Quote: The thermal stability of wild-type HigBHigA and HigB(L5ext)HigA were assessed using a Tycho NT.6 instrument (NanoTemper). Protein was heated at 0.1°C steps over a temperature range of 35°C to 95°C ...
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bioRxiv - Molecular Biology 2021Quote: Thermal stability was evaluated by nanoDSF using the Tycho NT.6 system (NanoTemper Technologies GmbH, München, Germany) 68 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescently labeled 100 nM NKR-P1 was mixed with dilution series of LLT1 or LLT1SIM and analyzed in standard capillaries in an NT.115 Monolith (NanoTemper) using 30% LED excitation power and 60% MST power ...
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bioRxiv - Molecular Biology 2021Quote: ... Nanobodies were labeled using the lysine reactive Monolith NT Protein Labeling kit RED-NHS (NanoTemper). As TCNB11 has a lysine residue in CDR2 ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were loaded into glass capillaries (NanoTemper, Munich, GER) and MST measurements were performed at various values of LED power and MST power ...
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bioRxiv - Molecular Biology 2021Quote: ... and TCNB34 to TC:TCblR were performed on a monolith NT.115 instrument (NanoTemper)(18) ...
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bioRxiv - Molecular Biology 2021Quote: TCNB4 was covalently labeled with Red-NHS using the Monolith NT kit (Nanotemper technologies) and used in the cellular binding and uptake studies ...
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bioRxiv - Molecular Biology 2021Quote: ... Measurements were performed using a Monolith NT.115Pico instrument (NanoTemper Technologies) at an ambient temperature of 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Capillary forces were used to load the samples into Monolith NT.115 Premium Capillaries (NanoTemper Technologies). Measurements were performed using a Monolith NT.115Pico instrument (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Data were fitted to the Kd model using MO.Affinity Analysis software (NanoTemper) and were plotted using Prism 8.0.2 (GraphPad) ...
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bioRxiv - Molecular Biology 2021Quote: ... The onset of scattering and melting temperatures based on the change of intrinsic protein fluorescence (ratio 350 nm/330 nm) were calculated in PR.ThermControl software (NanoTemper).
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bioRxiv - Molecular Biology 2021Quote: ... Samples were loaded to glass capillaries and analysis was performed in Prometheus NT.48 (NanoTemper). Data points were recorded from 35– 65°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and run at 50 % excitation power on a Monolith NT.115 (NanoTemper Technologies) at a constant temperature of 25 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... KD was determined according to the law of mass action using the program MO Affinity Analysis (NanoTemper Technologies) and results were plotted using Prism (Graphpad).
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bioRxiv - Molecular Biology 2021Quote: ... the samples were loaded into premium coated capillary tubes (Nanotemper) before undergoing MST in a Monolith™ NT.115 instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... Capillary forces were used to load the samples into Monolith NT.115 Premium Capillaries (NanoTemper Technologies). Measurements were performed using a Monolith Pico instrument (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Affinity Analysis software (NanoTemper Technologies). Graphs were plotted using GraphPad Prism 8.4.3 software.
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bioRxiv - Molecular Biology 2021Quote: ... Measurements were performed using a Monolith Pico instrument (NanoTemper Technologies) at an ambient temperature of 25 °C ...
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bioRxiv - Microbiology 2021Quote: EsxA sample purity was confirmed using tryptophan fluorescence (NanoTemper Tycho). Pure MBP was used as a negative control (sample impurity control ...
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bioRxiv - Molecular Biology 2021Quote: ... MST was performed in Monolith NT.115 (NanoTemper) at 22°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µL reactions were incubated at room temperature for 5 min and 5 µL of the mixture was loaded into NT.115 capillaries (NanoTemper). MST was performed in Monolith NT.115 (NanoTemper ...
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bioRxiv - Molecular Biology 2021Quote: ... KD measurement (KD model) and statistic evaluation were performed automatically with default parameters using MO.Affinity software (NanoTemper).
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bioRxiv - Biochemistry 2021Quote: ... and RBD-71 measured using the Tycho NT.6 (NanoTemper).
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bioRxiv - Biophysics 2021Quote: ... PR.ThermControl V2.1.2 software (NanoTemper Technologies) was used to control the device ...
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bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
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bioRxiv - Biophysics 2021Quote: ... Melting curves and Tm values were generated by PR.ThermControl V2.1 software (NanoTemper Technologies) using the first derivative of the fluorescence at 330 nm.