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Citations for Agilent :
1501 - 1550 of 1579 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Variants were introduced into the wild type constructions using the Quick-change II XL site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA). Presence of an abnormally spliced transcript associated with the decrease of the normal transcript was considered as “Impact on splicing” ...
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bioRxiv - Molecular Biology 2022Quote: Metabolite profiling analysis was completed using ion-paired reversed phase liquid chromatography using an HPLC (1290 Infinity II, Agilent Technologies, Santa Clara, CA) coupled to a triple quadrupole mass spectrometer (6470 ...
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bioRxiv - Cell Biology 2022Quote: ... were introduced into the plasmid pED-FLAG-LMAN1 and pcDNA-MCFD2-Myc separately using the QuickChange site-directed mutagenesis II XL kit from Agilent (Santa Clara, CA). FVIII mutant constructs Δ807–816 (deletion of amino acids 807-816 of FVIII ...
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bioRxiv - Cell Biology 2022Quote: ... or C166A/C180A in the GABAA receptor α1 subunit) were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). A FLAG tag was inserted between Leu31 and Gln32 of the α1 subunit and between Asn28 and Asp29 of the β2 subunit of GABAA receptors by using QuikChange II site- directed mutagenesis ...
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bioRxiv - Cancer Biology 2022Quote: ... The locus-specific HDR donors were generated by PCR amplification of the MACHETE bicistronic cassette using a high-fidelity DNA polymerase (Herculase II, Agilent or Q5, NEB). PCR fragments were column purified (Qiagen ...
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bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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bioRxiv - Physiology 2022Quote: ... The dynamin-2 mCherry mutations A618T and S619L were constructed by site directed-mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Texas, USA). (DYN2 isoform aa ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of CH4 was quantified by gas chromatography with flame ionization detection (Hewlett Packard HP 5890 Series II Gas Chromatograph, Agilent Technologies, California, USA). Air pressure was also monitored at each sampling point by a portable pressure meter (GMH 3100 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.7 µm) fitted in a 1290 Infinity II HPLC linked to an Ultivo (G6465) triple-quadruple mass spectrometer (all from Agilent Technologies, Waldbronn, Germany). Electrospray ionization (ESI+ ...
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bioRxiv - Physiology 2023Quote: ... Point mutations were generated using the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: The characterization of EPP and UPP were performed by size-exclusion high performance liquid chromatograph (SE-HPLC) using an Agilent 1290 Infinity II LC system (Agilent Technologies, http://www.agilent.com). Twenty microliters of the EPP and UPP extracts were separately injected into a Bio SEC-5 column (4.6×300 mm ...
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bioRxiv - Genetics 2023Quote: The characterization of gliadins was performed by reverse-phase high performance liquid chromatograph (RP-HPLC) using an Agilent 1290 Infinity II LC system (Agilent Technologies, http://www.agilent.com). Twenty microliters of the gliadin extracts were injected into a C18 reversed-phase Zorbax 300 StableBond column (4.6×250 mm ...
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bioRxiv - Neuroscience 2023Quote: The efficiency of Dex encapsulation in Lipo and its subsequent release was evaluated with high-performance liquid chromatography (HPLC) using the 1260 Infinity II LC System (Agilent Technologies, California, US). A Luna® 5 μm C18(2 ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutations were introduced into ATP8B2 and ATP8B4 using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and/or the SLiCE cloning method (Zhang et al ...
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bioRxiv - Cancer Biology 2023Quote: Targeted lipidomics was performed on a 1290 Infinity II UHPLC coupled to a 6470 QQQ mass spectrometer via AJS ESI source (Agilent, Santa Clara, USA) in positive ionization mode ...
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bioRxiv - Cell Biology 2023Quote: ... The human GABAA receptor α1 subunit missense mutations (S76R, R214C, D219N, G251D, P260L, M263T, T289P, and A322D) were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). Enhanced cyan fluorescent protein (CFP ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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bioRxiv - Physiology 2023Quote: ... Samples (4 µL) were injected via an Infinity II Bio liquid chromatography system into a 6495C triple quadrupole mass spectrometer (both Agilent Technologies, Waldbronn/Germany). Metabolites were separated on an Acquity BEH Amide HILIC column (1.7 µm ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was done with a Agilent AriaMX qPCR machine according to the protocol for Brilliant II SYBR QPCR Low ROX Mstr Mx (Agilent, catalog number 600830). IKZF1 qPCR primers ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Cell Biology 2019Quote: ... First peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Systems Biology 2019Quote: ... 20% of the total mixture was incubated at 37°C with PNGaseF and fractionated into 16 high pH reversed phase fractions using a 1260 Infinity II HPLC (Agilent Technologies, Santa Clara, CA) with a 4.6 × 150 mm XBridge C18 column and a 30 minute gradient (mobile phase A ...
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bioRxiv - Neuroscience 2019Quote: ... A 22-bp sequence containing the translation initiation site of npba in this BAC clone was replaced by a 2136-bp DNA cassette containing the humanized Renilla reniformis GFP II-coding sequence (Agilent Technologies, Santa Clara, CA), bovine growth hormone polyadenylation signal ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Biochemistry 2021Quote: ... Preparative purification by HPLC was carried out on an Agilent 1260 Infinity II device using an Eclipse XDB-C18 (Agilent, 21.2 x 250mm, 7µm) reversed-phase column ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Microbiology 2021Quote: Reverse phase ultra-high performance liquid chromatography (UHPLC) analysis was performed on Agilent 1290 Infinity II UHPLC system controlled using OpenLab CDS software (Agilent Technologies, Santa Clara, CA). The concentration of protein was determined using a Poroshell 120 SB-Aq column (2.1 x 50 mm ...
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bioRxiv - Microbiology 2021Quote: ... two stop-codons were introduced directly after the start-codon of vpu using the QuikChange II XL site-directed mutagenesis protocol (Agilent Technologies, Santa Clara, CA). The presence of the desired mutations was determined by automated DNA sequencing ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed on diluted lysis product (1:20 dilution) and 25 µl of PCR reaction mix according to the manufacturer instruction (Herculase II Fusion DNA Polymerase, Agilent Technologies, Les Ulis, France) using the following PCR primers ...
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bioRxiv - Developmental Biology 2022Quote: ... A 40 µl aliquot of the purified sample was used for analysis with the LC-MS/MS system (Sciex API6500+ triple quad mass spectrometer hyphenated to an Agilent 1290 Infinity II UHPLC). Chromatographic separation was performed on a biphenyl stationary phase (Restek Raptor Biphenyl 100×2.1 ...
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bioRxiv - Cell Biology 2022Quote: ... I15K/R54P) were generated by mutagenesis of pcDNA-3XFLAG-TRIM25 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA), while the TRIM25-PTAA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... The resulting solutions were further analysed by high-performance liquid chromatography using a 1290 Infinity II UPLC instrument (Agilent Corp., Santa Clara, CA, USA) coupled with an AB SCIEX Triple Quad™5500 (AB SCIEX ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Biophysics 2019Quote: Ser or Thr to Cys mutations were introduced into pET30b GluBP via site-directed mutagenesis according to the QuikChange II XL protocol (AGILENT TECHNOLOGIES, Santa Clara, CA) using the following primers:
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bioRxiv - Biochemistry 2020Quote: ... In the 1st step YeGFP (referred to as GFP) was amplified using GFP specific primers and Mutazyme II polymerase (Agilent Technologies; Cat no. 200550) in order to incorporate 7-11 mutations per kb of plasmid ...
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bioRxiv - Physiology 2020Quote: ... an acetonitrile precipitation was performed prior to sample analysis by an Agilent 1290 Infinity II HPLC with an Agilent 6550 iFunnel Q-TOF mass spectrometer for detection (Agilent, Santa Clara, CA, USA). Gefitinib and deuterated gefitinib (gefitinib-d6 ...
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bioRxiv - Genetics 2020Quote: ... and their identities were confirmed using an Agilent 1290 Infinity II liquid chromatography system coupled with Agilent 6530B Quadrupole time-of-flight mass spectrometry (Agilent Technologies, Santa Clara, CA) in a negative ion polarity mode ...
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bioRxiv - Cancer Biology 2019Quote: The pEGFP-N1-CELF1 putative eIF4E binding mutants were generated via site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, La Jolla, CA) and primers listed in Supplementary Table 2 ...
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bioRxiv - Immunology 2021Quote: ... TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid phapXVENUS-hph (Gsaller et al., 2014) was used as template and modified using QuikChange II site-directed mutagenesis kit (Agilent for K38E and R50A) or Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The α10W55T mutant subunit was produced as previously described (Boffi et al., 2017) using Quick change XL II kit (Stratagene, La Jolla, CA, USA). The chimeric α10x construct was generated in two steps ...
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bioRxiv - Neuroscience 2022Quote: ... TRP and serotonin were quantified using reverse phase ultra-high-performance liquid chromatography (UHPLC) coupled to a triple quadrupole mass spectrometer (1290 Infinity II LC System, 6470 Triple quadrupole, Agilent Technologies, Santa Clara, CA). Five-microliters of samples were injected onto a Vanguard HSS T3 Pre-column that was connected to an Acquity HSS T3 analytical column ...
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bioRxiv - Biochemistry 2024Quote: A genetic library was constructed based on the metX and metY genes with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA), adjusted to produce an average of 4 nonsynonymous mutations per gene ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...