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Citations for Agilent :
1251 - 1300 of 1579 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... To introduce the g.26652G>A mutation we used the Quick-change II XL Site-directed Mutagenesis Kit (Agilent Technologies, USA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The PGBD1 ORF was amplified from a cDNA library prepared from HeLa cells using DNA polymerase Pfu Ultra II Fusion HS (Agilent Technologies) and PGBD1 Forw (5’-ATGTATGAAGCTTTGCCAGGC-3’ ...
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bioRxiv - Genomics 2019Quote: ... followed by three 10 min incubations in 500 μl wash buffer II at 65°C) were performed according to the SureSelect Target enrichment protocol (Agilent Technologies). After the final wash ...
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bioRxiv - Immunology 2021Quote: ... The amplified DNA purified and subsequently mutagenized by error-prone PCR (ePCR) via the GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with a target nucleotide mutation frequency of 0-4.5 mutations/kb ...
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bioRxiv - Neuroscience 2019Quote: ... The GFP probe was generated from a 733-bp fragment corresponding to nucleotides 733-1465 of the phrGFP II-1 mammalian expression vector (Agilent Technologies). The npba and npbb probes used were the same as those used for single in situ hybridization ...
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bioRxiv - Cancer Biology 2019Quote: ... and amplified with the primers to yield an amplicon roughly 150-250 base pairs in length using Herculase II Fusion Polymerase (Agilent #600675). The PCR protocol involved a 2 minute denaturation at 95C ...
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bioRxiv - Genetics 2019Quote: ... All qRT-PCR experiments were performed on diluted cDNA (1:5 in nuclease-free water) using the Brilliant II SYBR Green qPCR Master Mix (Agilent Technologies) and the 7900HT Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... LC-MS/MS (MRM mode) analyses were performed with a MS model QTRAP® 6500 (ABSciex) coupled to an LC system (1290 Infinity II, Agilent). Analyses were achieved in the negative (PA ...
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bioRxiv - Genomics 2019Quote: ... fragments with UMIs were generated using 200 ng starting material (purified by ethanol precipitation) in 2 cycles of PCR with Herculase II Fusion DNA Polymerase (Agilent Technologies) using an equimolar mixture of P023poolseqNN-primers (1 mM final concentration ...
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bioRxiv - Developmental Biology 2019Quote: ... truncated and deletion versions of RAB13 3’UTR were generated by PCR using 0.3 μM sequence-specific primers (Extended Data Table 2) and Platinum Pfx DNA polymerase or using QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions and introduced into the pcDNA3-HBB-24XMS2SL-MCS using the NheI and XhoI sites.
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bioRxiv - Genetics 2019Quote: ... The PAM sequences in the c(3)G gene were mutated using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent Technology). The bases changed are in bold above ...
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bioRxiv - Biochemistry 2021Quote: ... and PcrA V448C were generated by site-directed mutagenesis of the wild type construct using the Quikchange II XL kit (Agilent Technologies). In the last gel filtration chromatography of PcrA V448C ...
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bioRxiv - Genomics 2021Quote: ... Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent, 300385) using PCR generated DNA template produced from gDNA isolated from a wild type S.pombe strain (YP71 ...
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bioRxiv - Cell Biology 2020Quote: ... To construct a plasmid encoding GAS6 tagged with Myc-His (pSecTag2-hGAS6-MycHis) the stop codon from pSecTag2-hGAS6 was removed using the QuickChange II XL Site-Directed Mutagenesis kit (Agilent Technologies). Next ...
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bioRxiv - Microbiology 2021Quote: Mutations in AdMarRars were introduced by site-directed mutagenesis using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The mutagenic oligonucleotides used for both strands and the respective changes introduced (underlined ...
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bioRxiv - Bioengineering 2020Quote: ... respectively in ThuE were designed by site-directed mutations in optical sucrose sensors by using the QuickChange II Site-Directed Mutagenesis (SDM) Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... We confirmed their identities and quality using an Agilent 1290 Infinity II high-performance liquid chromatography (HPLC) system coupled with an Agilent 6530B Quadrupole time of-flight mass spectrometry (Agilent Technologies) in negative ion polarity mode.
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bioRxiv - Cell Biology 2021Quote: ... the beads were then washed three times and submitted to the Washington University Proteomics Core Lab for liquid chromatography-mass spectrometry (1260 Infinity II Bio-Inert LC System, Agilent Technologies).
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Physiology 2020Quote: ... by site-directed mutagenesis of a pCI-NaV1.5 plasmid containing the human NaV1.5 hH1C cDNA (30) (NCBI Reference Sequence NM_000335) using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Sant Clara, CA) or the Q5 Site-Directed Mutagenesis kit (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Plant Biology 2021Quote: ... CA- and DN-RABG3 variants were generated by site directed mutagenesis with QuickChange Lightning II Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturers’ instructions ...
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bioRxiv - Biochemistry 2021Quote: ... two arginines at position 682 and 683 were changed to alanines using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) and the plasmid pIB-spike-V5 as a template ...
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bioRxiv - Cell Biology 2021Quote: ... A gene fragment encoding three copies of the 9-amino-acid HA epitope followed by EcoR1 and XbaI restriction sites was inserted using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies). The resulting GSPK-HA transgene plasmid (13,468 bp ...
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bioRxiv - Cell Biology 2021Quote: ... We performed mutagenesis with the primers (Table S1) designed for the R397W mutation according to the manufacturer’s instruction (QuikChange II Site-Directed Mutagenesis Kit, Agilent Technologies, USA). Upon reaching 50-60% density ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutations of the phosphorylation sites were created using similar methods as the Quickchange® II XL kit from Agilent technologies by using the primers listed below to recreate the whole vector via PCR with the new mutation in place ...
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bioRxiv - Cancer Biology 2019Quote: ... The SfiI site in mouse Tns4 cDNA was mutated using the QuikChange II XL mutagenesis kit (Agilent, Santa Clara, CA, USA) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... or turboGFP-MCMBPΔNLS (bipartite NLS was identified using cNLS Mapper35 generated by site directed QuickChange II XL Site-Directed Mutagenesis kit (Agilent, 200522) with primers (forward ...
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bioRxiv - Microbiology 2020Quote: pPB-NSP5-SV5-Csy4-H29A and pPB-SV5-Csy4-H29A plasmids carrying two nucleotides substitutions C82G and A83C in the Csy4 ORF were generated by QuikChange II Site-Directed Mutagenesis (Agilent Technologies) from the pPB-NSP5-SV5-Csy4 and pPB-SV5-Csy4 respectively using Csy4-H29A-FOR and Csy4-H29A-REV primers (Figure Supplement Data 1).
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bioRxiv - Microbiology 2020Quote: ... 1 μL of cDNA was used for SYBR green qPCR using Brilliant II SYBR Green QPCR Master Mix with Low ROX (Agilent #600830) in Bio-Rad Hard Shell PCR 96-well plates (Bio-Rad #64201794 ...
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bioRxiv - Microbiology 2020Quote: ... we carried out site direct mutagenesis of pCR-ompT-GFP according to the Quikchange II site-directed mutagenesis kit (Agilent Technologies).
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bioRxiv - Biophysics 2021Quote: The mutant A350P mutant was generated using pET-LA as template by site directed mutagenesis (QuickChange II XL Site Directed Mutagenesis Kit, Agilent-USA). The primers used for the same were a ...
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bioRxiv - Biophysics 2020Quote: ... The desired base pairs coding for Cys were introduced using overlapping primers with the QuikChange II site-directed mutagenesis kit (Agilent Technologies) and were confirmed by DNA sequencing (GENEWIZ ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were loaded on a Superdex 200 Increase 10/300 GL column (Cytiva) run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting eight vectors were then subjected to site-directed mutagenesis (QuickChange II XL Kit; Agilent Technologies, La Jolla, CA, USA) to introduce the codons of interest ...
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bioRxiv - Biochemistry 2022Quote: ... and C507A CAPN5 mutations and the C506A CAPN6 mutation were introduced with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The C246A and C512A mutations and the C495A/C507A/C512A triple mutation were introduced by PCR amplification of phCAPN5-3×FLAG with 5’-phosphorylated primers incorporating the respective mutations ...
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bioRxiv - Biophysics 2022Quote: ... The samples were loaded on a Superdex 200 10/300 Increase column (Cytiva) at a temperature of 20°C run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 ml/min ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... a stop codon was introduced into a previously generated pDONR-CP110L (containing a full length CP110 cDNA) (Franz et al., 2013) by site directed mutagenesis using Quikchange II XL mutagenesis kit (Agilent technologies). This was then recombined with the pUbq-empty vector (This paper ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Biochemistry 2022Quote: ... The D454N mutation was introduced by site-directed mutagenesis using the QuickChange™ II XL site-directed mutagenesis kit (Agilent technologies). An overlap extension PCR strategy was used to insert the 3C protease site (LEVLFQGP ...
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bioRxiv - Cancer Biology 2022Quote: ... Sirt1 H355A mutation was generated by performing directed mutagenesis on this vector using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis for Cys238>Ala active site substitution in scpA was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using oligonucleotides MP_scpA1 and MP_scpA2 [49].
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bioRxiv - Plant Biology 2021Quote: ... Hormone analysis was performed with a LC/MS system consisting of UHPLC 1290 Infinity II coupled to 6495 Triple Quadrupole Mass Spectrometer (Agilent, USA).
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bioRxiv - Cancer Biology 2020Quote: ... LTM Series II Fast GC Module, 7000C GC-MS/MS Triple Quadrupole, 7693 Autosampler and 7697A Headspace Sampler, all Agilent Technologies). The prepared samples were injected into the GC using the following conditions (1 µL pulsed split-less injection at 235 °C ...
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bioRxiv - Bioengineering 2021Quote: The integrated sgRNA library was PCR amplified and separately barcoded for each collected population with Herculase II Fusion DNA Polymerase (Agilent 600677). Samples were then pooled and sequenced on an Illumina NextSeq flow cell with aligned read counts as follows for each screen:
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bioRxiv - Molecular Biology 2020Quote: The plasmid pUHD-FLAG-H3.3K56R and pUHD-FLAG-H3.3K56Q were constructed using a Quick Change II Site-Directed Mutagenesis Kit (Agilent, CA, United States), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... fructose in PJ medium was determined by liquid chromatography - tandem mass spectrometry (LC–MS/MS) technique (1260 Infinity II Series Liquid Chromatograph, Agilent, USA). The InfinityLab Poroshell 120 EC-C18 column (Agilent ...