Labshake search
Citations for Agilent :
6551 - 6600 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... GST-Rab5C213A and GST-Rab5C212A/C213A in pGEX-2T were constructed by mutating GST-Rab5 (wild type) in pGEX-2T using a Quik-Change Site-Directed Mutagenesis Kit (Agilent Technologies, San Diego, USA). The mutated Rab5 genes were then cloned into the pCMV-HA vector ...
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bioRxiv - Evolutionary Biology 2024Quote: A phage display PAI-1 variant library was constructed on the I91L PAI-1 background using error prone PCR as previously described (7, 8) with the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) using primers that preserved the AscI and NotI restriction sites ...
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bioRxiv - Genetics 2024Quote: Microdeletions and candidate variants (Supplementary Table S1) were introduced into the wild type (WT) construct by site-directed mutagenesis using the QuikChange Lightning Kit (Agilent, Santa Clara, CA, USA). Mutant minigenes were confirmed by sequencing (Macrogen) ...
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bioRxiv - Cancer Biology 2021Quote: Deletion mutants of two TCF4 binding sites (TBE1 and TBE2) on the STUB1 promoter were generated as previously performed [25] using the Quickchange XL Site directed mutagenesis kit (Agilent technologies, Santa Clara, CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the material was loaded onto denaturing PolyAcrylamide Gel Electrophoresis (dPAGE) at 12% or analyzed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). Of course ...
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bioRxiv - Molecular Biology 2022Quote: ... Ser181Ala;Thr100Ala double mutant and Ser181Ala;Thr100Ala;Thr692Ala triple mutant were prepared using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... One microliter of the purified RNA solution was used for electrophoresis using a Bioanalyzer 2100 with Agilent RNA nano or pico kit (Agilent Technologies, Santa Clara, CA, USA) to check for the quality.
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bioRxiv - Molecular Biology 2021Quote: ... the steps were as follows: a) quality control using the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA), b ...
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bioRxiv - Molecular Biology 2020Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (cat # 5067-1513, Agilent Technologies Inc., Santa Clara, USA). Lowest RIN was 9.3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the quality and quantity of that pool was assessed via electrophoresis (High-Sensitivity DNA Kit and Agilent Bioanalyzer; Agilent Technologies, Santa Clara, CA, USA), real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...
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bioRxiv - Immunology 2021Quote: ... RNA quantification and quality assessments were performed by ultraviolet–visible spectrophotometry (Nanodrop Technologies, Wilmington, DE) and the RNA 6000 Nano Chip Kit with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA quality of all samples reached a RNA integrity number (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (cat # 5067-1513, Agilent Technologies Inc., Santa Clara, USA). Lowest RIN was 9.1 ...
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bioRxiv - Genetics 2022Quote: ... The purified RNA quality was evaluated through capillary electrophoresis in the Bioanalyzer 2100 with an RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA) and quantified using a Nanodrop spectrophotometer ...
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bioRxiv - Genomics 2020Quote: ... The quality and yield of the prepared libraries were assessed using a high sensitivity Small DNA Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The length of the eluted DNA fragments was measured on a Femto Pulse using the Agilent Genomic DNA 165 kb kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2022Quote: ... for 1 hour at room temperature followed by treatment with HRP-conjugated anti-mouse secondary antibody (DAKO Envision Plus HRP kit, Dako Denmark A/S, Glostrup, Denmark) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... RNA yield was quantified with a NanoDrop Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and RNA integrity was assessed with Bioanalyzer 2100 RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer protocol and complementary DNAs were generated using an AccuScript High Fidelity 1st Strand cDNA Synthesis kit (Agilent Technologies, Santa Clara, VA, USA). A quantitative PCR was performed using a QuantStudio™ 3 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... RNA integrity was defined by capillary electrophoresis with an RNA 6000 Nano Lab-on-a-Chip kit and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Only if the integrity number values of RNA were more than 6 ...
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bioRxiv - Microbiology 2020Quote: Single point SCoV2-NTD-Fc N-linked glycosylation site mutants (N61, 74, 122, 149, 165, 234, 282Q) were generated using a QuikChange site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) to change the respective asparagine (N ...
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bioRxiv - Microbiology 2020Quote: ... The synthesis and fluorescence labeling of cDNA followed a strand-specific method using the FairPlay III Microarray Labeling Kit (Agilent Technologies, Santa Clara, CA, USA) and actinomycin D (Calbiochem ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Immunology 2020Quote: ... and Ii L17A was subsequently used as template for PCR quick change mutagenesis (all reagents used were included in the kit; QuickChange® Site-Directed Mutagenesis (Stratagene, La Jolla, CA, USA)) in order to generate the AP-3 binding motif RRP 21 ...
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bioRxiv - Immunology 2021Quote: ... was performed on an Illumina HiSeq 4000 machine using libraries prepared with a SureSelectXT Mouse All Exon capture kit (Agilent Technologies, Santa Clara, CA, USA), to produce 150 paired end reads sufficient for a mean bait coverage of at least 150x ...
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bioRxiv - Immunology 2020Quote: ... The RNA Integrity Number (RIN) of all RNA samples were determined using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 nano kit (Agilent Technologies, Santa Clara, CA, USA). A minimum RIN of 7.5 was used as the standard for inclusion in the gene expression study ...
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bioRxiv - Cancer Biology 2022Quote: ... of matched-PBMC DNA was fragmented using the SureSelect XT HS and XT Low Input Enzymatic Fragmentation Kit following the manufacturer’s instructions (Agilent, Santa Clara, USA, cat # 5191-4080). Molecular-barcoded libraries were constructed following the SureSelect XT HT targeted enrichment protocol for Illumina paired-end multiplexed sequencing libraries (Version A1 ...
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bioRxiv - Cancer Biology 2022Quote: Deletion of TBE consensus sites in the FOXM1 promoter was accomplished using the QuickChange XL Site-Directed Mutagenesis44 kit (Agilent Technologies, Santa Clara, CA, USA) by following manufacturer’s instructions and was then cloned into the pGL3 basic vector ...
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bioRxiv - Pathology 2022Quote: ... The RNA Integrity Number (RIN) of all samples was quantified using an Agilent 2100 Bioanalyser and Agilent RNA 6000 nano kit (Agilent Technologies, Santa Clara, CA, USA). RIN values above 7.5 were used as the inclusion criterion for subsequent gene expression analysis ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality was evaluated by measuring the RNA integrity number (RIN) using an Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The EGFP and mCherry dynamin-2 mutations A618T and S619L were constructed by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). Neuropeptide Y fused to mCherry (NPY-mCherry ...
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bioRxiv - Neuroscience 2023Quote: ... We constructed the HPRT1-I42T cDNA from this clone by site-directed mutagenesis using the Quick Change II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). Primers sequences used to create the mutation are shown in Table S3 ...
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bioRxiv - Cancer Biology 2024Quote: ... The quality and integrity of the total RNA were determined using the Agilent 2100 Bioanalyzer and the RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, United States). A sequencing RNA library was constructed by performing a 3′-end linker ...
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bioRxiv - Genetics 2024Quote: ... Whole exome sequencing was performed on genomic DNA extracted from the peripheral lymphocytes of the patients and their families using the SureSelect Human All Exon Kit V6 (Agilent Technologies, Santa Clara, CA, USA) with sequencing on the NovaSeq 6000 platform (Illumina ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent, catalog no. DNF-474-1000). Genes with low expression value (mean FPKM value<0.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The quality of the total RNA extracted from target cells was evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA integrity was determined based on the RNA Integrity Number (RIN ...
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bioRxiv - Microbiology 2023Quote: K65R and M184V mutations in RT were generated by site-directed mutagenesis on an HXB2 or LAI HIV-1 backbone using the QuikChange XL site-directed mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA) according to the manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The library was quality controlled by analysis on an Agilent 2000 Bioanalyzer with the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA, USA) for fragment sizes of ca ...
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bioRxiv - Cancer Biology 2022Quote: A library of the entire genomic sequence of all 50 known genes (electronic supplementary Table S1) was prepared using HaloPlex Target Enrichment kits (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantity and purity were determined using a NanoDrop™ spectrophotometer (ND-1000, ThermoScientific™, Delaware, USA) and an Agilent 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies, CA, USA) using the 2100 Expert Software to calculate RNA Integrity Number (RIN ...
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bioRxiv - Pathology 2023Quote: ... A mutant minigene with the c.1066-11G>A variant was generated by site-directed mutagenesis with QuikChange Lightning Kit (Agilent Technologies, Santa Clara, CA, USA). Sanger sequencing confirmed the identity of the constructs ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 3 to 6 sections of each tumor were also immunostained for Ki67 (mouse anti-ki67, BD, San Jose CA, USA; blocking reagent, M.O.M ImmPRESS kit, Vector Laboratories, Burlingame CA, USA; liquid DAB+, Dako North America Inc, Carpinteria CA, USA), digitized (Nanozoomer ...
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bioRxiv - Zoology 2024Quote: ... The quality and quantity of the libraries were checked on an Agilent 2100 Bioanalyzer using the Agilent DNF-935 Reagent Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were pooled to equimolar amounts ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality and integrity were assessed via chip gel electrophoresis with a 2100 Bioanalyzer and an RNA Nano chip kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Mutation of the catalytic active site at position 138 (Ser-138 to Ala-138) of RLip was introduced via the QuikChange II XL site-directed mutagenesis kit (Agilent technologies, Cat-No.: 200521-5) according to the manufacturer’s instructions using the using Forward primer 5’-CTTTTAACACCAGCGGCCACGCGCTGGGCGCGATTATC-3’ and Reverse primer 5’-GATAATCGCGCCCAGCGCGTGGCCGCTGGTGTTAAAAG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... Prokaryotic 16S rRNA gene (V3V4 hypervariable region) amplicon sequence libraries were prepared using Herculase II Fusion DNA Polymerase and the Nextera XT Index V2 Kit (Agilent Technologies, Santa Clara, CA, USA) with primers Bakt_341F (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Immunology 2024Quote: ... the relevant assay media was used to make stock solutions of the different compounds associated with the Seahorse XF Mito Stress Test Kit (Agilent Technologies, Catalog No. 103015-100), the Seahorse XF Real-Time ATP Rate Assay Kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: The A30P αSyn plasmid was prepared by site-directed mutagenesis of the pT7-7 WT αSyn plasmid using a QuikChange II Site-Directed Mutagenesis Kit (Agilent, Agilent Technologies, Santa Clara, CA, USA), the primers ...