Labshake search
Citations for Agilent :
6101 - 6150 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The quality and quantity of all libraries were checked using Agilent’s Bioanalyzer 2100 and DNA 7500 kit (Agilent Technologies, 5067-1506).RNAseq analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in primary antibodies overnight at 4 °C (Figure S2) and in secondary antibody-horseradish peroxidase complex (REAL EnVision detection kit, Dako #K5007) for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality of libraries were checked on a Bioanalyser with the use of a High sensitivity DNA kit (Agilent, 5067-4626). The libraries were sequenced on an Illumina Next-Seq 550 system.
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bioRxiv - Microbiology 2024Quote: ... RNA quality was assessed using the Agilent 2100 Bioanalyzer system and the RNA 6000 Pico kit (Agilent Technologies, Santa Clara, USA).
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bioRxiv - Neuroscience 2024Quote: ... Library molarity was determined by measuring the library size using the FemtoPulse instrument and the Genomic DNA 165 kb Kit (Agilent Technologies) and the library concentration using Qubit Fluorometric Quantitation and dsDNA High sensitivity assay (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: Quantification and quality of all libraries were assessed on a Bioanalyzer 2100 using High-Sensitivity DNA Reagent Kit (Agilent, 5067-4626). Single-cell gene expression and ATAC replicates were separately pooled to 2nM ...
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bioRxiv - Biophysics 2024Quote: ... Site-directed mutagenesis was performed according to the instructions accompanying the Quik-Change® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). The principal vector used for most A ...
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bioRxiv - Biophysics 2024Quote: ... Site-directed mutagenesis was performed according to the instructions accompanying the Quik-Change® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). The principal vector used for most A ...
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bioRxiv - Biophysics 2024Quote: ... each mutation was individually introduced into the plasmid carrying the wild-type Tv0924 sequence using the QuikChange site-directed mutagenesis kit (Agilent Technologies). The mutation was confirmed by DNA sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... The NDec1-98 mutant was prepared using purified N-His6 Dec plasmid DNA from a DH5α bacterial culture that was obtained using a StrataPrep plasmid miniprep kit (Agilent Technologies). Replacing codon 99 with a stop codon was accomplished by site-directed mutagenesis ...
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bioRxiv - Genomics 2024Quote: ... Analysis of ATAC-seq library quality was done with Agilent 2100 Bioanalyzer using High Sensitivity DNA Analysis kit (Agilent, #5067-4626). For ChIP-seq two biological replicate samples were sequenced with Illumina NextSeq 500 (75SE) ...
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bioRxiv - Genomics 2024Quote: ... Analysis of scRNA-seq library quality was done with Agilent 2100 Bioanalyzer using High Sensitivity DNA Analysis kit (Agilent, #5067-4626). scRNA-seq libraries were sequenced with Illumina NextSeq 2000 (100PE).
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bioRxiv - Genomics 2024Quote: ... HMW DNA was further qualified using the FP-1002 Genomic DNA 165-kbp kit for FEMTO Pulse systems (Agilent Technologies, USA). Library preparation was performed using the native ligation sequencing kit (SQK-LSK109 ...
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bioRxiv - Genomics 2024Quote: ... One-μg aliquots of total RNA were labelled using the Low RNA input fluorescent linear amplification (LRILAK) PLUS kit (Agilent Technologies); internal standards came from the Two-color RNA spike kit (Agilent technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... oligomycin (1 µM) and 2-deoxy-D-glucose (2-DG) (50 mM) (Seahorse Glycolysis Stress Test Kit, Agilent Technologies, 103020-100). Analyses were performed with Wave software 2.3.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... The latter was used as a template to generate pLVET-HA-K-RasG12D-IRES-GFP (HA-G12D) and pLVET-HA-K-RasG12D/G10L-IRES-GFP (HA-G12D/G10L) with QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent; # 210519). The sequences were confirmed by Sanger sequencing.
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bioRxiv - Systems Biology 2024Quote: ... The quality of the library was then assessed using the Agilent High Sensitivity DNA Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies). Quantification was done using Qubit (Qubit dsDNA High Sensitivity Assay Kit ...
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bioRxiv - Developmental Biology 2024Quote: miR-92b-3p binding sites in each 3’UTR were mutated using the QuikChange II XL site-directed mutagenesis kit (Agilent, #200517) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... A cysteine-313 to alanine (C313A) substitution of mPPARγ construct was generated with a mutagenesis kit (cat. no. 210518, Agilent, USA) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... and by using the Agilent RNA 6000 Pico Kit with a 2100 Bioanalyzer according to the manufacturer’s instructions (Agilent, CA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were determined with the XF Cell Mito Stress Kit (Agilent: #103015-100). All tRCC cell lines were seeded on a poly-L-lysine coated 96-well Seahorse plate (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) then anti-CD138 clone MI15 (M7228, Agilent Technologies Inc., Santa Clara, CA) was applied for 30 min at a concentration of 0.108 µg/mL at room temperature using Antibody Diluent (S302283-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... the DV200 quality metric of RNA from FFPE pancreas tissue was first verified using RNA 6000 Nano Kit (Agilent #5067-1511) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... mutation was generated by introducing a G to A point mutation at nucleotide 184 in the zebrafish Rac2 gene by QuikChange II Site-Directed Mutagenesis (Agilent kit) using primers 5’- cagtctgtcataatctttctgtccggctgtatccc-3’ and 5’-gggatacagccggacagaaagattatgacagactg-3’ (anti-sense).
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bioRxiv - Systems Biology 2020Quote: ... The resulting cDNA library was assessed for yield and purity using an Agilent High Sensitivity DNA kit for Agilent 2100 Bioanalyzer (Agilent Technologies, CA). Template preparation with the cDNA libraries followed by ISP enrichment was performed using the Ion OneTouch2 system with Ion PGM™ Hi-Q™ OT2 kit (Product No ...
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bioRxiv - Cancer Biology 2021Quote: ... Library concentrations as well as fragment size distributions were also analyzed by employing the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent: 5067-4626).
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted with Norgen single cell RNA purification kit (Norgen) and the quality was checked with a Bioanalyzer 2100 (Agilent, RIN >8.0). Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre) ...
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bioRxiv - Cell Biology 2021Quote: Measurements of aerobic respiration and glycolysis were conducted with the Seahorse Bioscience XFe-96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent #103015-100). Cells were seeded on XF96 cell culture microplates (Agilent #102416-100 ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were then quantified using a Nanodrop microvolume spectrophotometer (ng/μl) and quality was assessed using the Agilent High Sensitivity DNA Kit (Agilent -5067-4626) on a Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA of CFs/HVPs co-culture and conditioned CFs exposed to co-culture medium was extracted using the Absolutely RNA Miniprep Kit (Agilent Technologies, USA) according to the manufacturer’s instructions and 1 μg was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 150 base pair oligos were chemically synthesized using proprietary Agilent technology and probes were labeled with either Cy3 or Cy5 dyes using the Genomic DNA ULS Labeling Kit (Agilent, 5190-0419). 40 ng of LAD and non-LAD probes were combined with hybridization solution (10% dextran sulfate ...
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bioRxiv - Immunology 2021Quote: ... Following assessment of the quality of total RNA using Agilent 2100 bioanalyzer and RNA Nano Chip kit (Agilent Technologies, Santa Clara, CA), rRNA was removed from 2000 ng of total RNA with Ribo-Zero rRNA removal kit specific for Human/Mouse/Rat ...
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bioRxiv - Genetics 2021Quote: ... To reproduce the A-variant the following primers were used for SDM by site directed mutagenesis using QuickChange II site directed mutagenesis kit (Agilent Technologies, UK) using the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA quality was assessed using an Agilent 2100 Bioanalyzer and the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RNA was quantified using Qubit RNA BR Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... was fragmented to 200bp using a Covaris E Series and the resultant libraries were subjected to DNA Capture using SureSelect XT Mouse All Exon kit (Agilent, Stockport, Cheshire) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The T695A and T656A mutations were generated in WT-YME1L1 plasmid via QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) and confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
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bioRxiv - Genomics 2020Quote: ... Samples were then cleaned up using Qiagen RNeasy Mini kit columns and their integrity was checked on Agilent 2200 Bioanalyzer (Agilent Technologies, USA). A total of 133 samples were selected for RNA sequencing (74 liver and 59 head kidney samples ...
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bioRxiv - Genomics 2022Quote: ... and library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene, La Jolla, USA). Up to eight colonies containing the PCR product were selected and inoculated on Luria Bertani agar slants ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The cDNAs were then synthesized and their qualities assessed on the Agilent Bioanalyzer with High Sensitivity DNA kit (Agilent Cat# 5067-4626). cDNAs were then used for library preparation and the quality of the final libraries assessed on the Agilent Bioanalyzer with DNA 1000 kit (Agilent ...
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bioRxiv - Genomics 2020Quote: ... the fragmented product is purified and then processed to generate sequencing-ready libraries using the SureSelectXT Library Preparation kit (Agilent, CA, USA) following manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: The point mutations and modifications in the linker sequence between CiVSD and FPs were done using the QuickCjhange II XL site-directed mutagenesis kit (Agilent Technologies, USA). The sequence verification of the novel constructs was done using Tag/dye-termination method (W ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Biochemistry 2020Quote: The oxygen consumption rate (OCR) of LPO-exposed HLCs was measured using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100) on a Seahorse XF Analyser (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... The mutations to generate Asp276AlaSd and Tyr435HisSd were introduced by the QuikChange II Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...