Labshake search
Citations for Agilent :
6251 - 6300 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... the A3 sequence specifying amino acids 21-393 was amplified from pHD1344tub(PAC)-Nterm3V5-A3 by error prone PCR using the GeneMorphII Random Mutagenesis kit (Agilent, Cat # 200523) and primers that add AttB sites (Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis was performed on the pGAMA-YAP construct to create p-GAMA-YAP-S127A using the QuikChange Lightning kit (Agilent Technologies #210513). Forward primers used for the Ser-to-Ala substitution was as follows ...
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bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2023Quote: ... RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer kit for total eukaryotic RNA (Agilent Technologies, Santa Clara, CA, USA). Samples underwent library preparation and sequencing at the UC Berkeley Functional Genomics and Vincent J ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control of the final libraries was completed on the Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent, 5067-4626) to determine the average library sizes ...
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bioRxiv - Cell Biology 2023Quote: ... Most missense and deletion mutations were inserted by site-directed mutagenesis using the QuikChange Lightning Kit (Agilent Technologies, Santa Clara, CA, USA) in combination with appropriate oligonucleotides (Supplements ...
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bioRxiv - Neuroscience 2023Quote: ... The quality of the extracted RNA was assessed by the 2100 Bioanalyzer Instrument using Agilent RNA 6000 Pico kit (Agilent, CA, USA) and an average integrity quality number (RIN ...
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bioRxiv - Genomics 2023Quote: ... Appropriate fragment size distributions were confirmed by running each sample on an Agilent Bioanalyzer using the DNA High-Sensitivity Kit (Agilent, #5067-4626).
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bioRxiv - Immunology 2023Quote: ... and size distribution was assessed using the Agilent High Sensitivity DNA Kit on the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Sequencing was performed using an Ion 540™ chip and the 540™ Chef kit on an Ion GeneStudio S5 sequencer.
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration and the RNA integrity number (RIN) were determined using a Bioanalyzer 2100 instrument and an Agilent RNA 6000 Pico Kit (Agilent 5067-1513).
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bioRxiv - Biophysics 2023Quote: ... the following HIV-1 IN mutants were prepared using the QuikChange II XL site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA): E138K ...
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Genetics 2023Quote: ... and Illumina adapters were added (second PCR) in a nested PCR manner using Agilent Herculase II Fusion DNA Polymerase Kit (Agilent, Cat # 600679). The PCR products were purified and sequenced using an Illumina NextSeq ...
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Genomics 2023Quote: ... All library validations were performed on a 2100 Bioanalyzer with High Sensitivity DNA kit according to manufacturer’s recommendations (Agilent Technologies, 5067-4626).
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was determined with an RNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5576, 5067-5578, 5067-5577) and only RIN scores of 8 or higher were used for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... A sequence coding for the MAAAPQKC peptide was inserted in place of the first ten codons of the ermCL sequence using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Inc). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutants of KflaHY2 were generated in the pET28a_KflaHY2 plasmid using the QuikChange® Lightning kit (Agilent, Santa Clara, California, USA) employing primers listed in Table S1 ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Genomics 2023Quote: ... cDNA was diluted to approximately 3 ng/μL and run on the 2100 Agilent Bioanalyzer using the High Sensitivity DNA Kit (Agilent 5067-4626). The measured fluorescence units in Fig 1b have been corrected for the dilution factor.
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bioRxiv - Plant Biology 2023Quote: ... Site directed mutagenesis was performed using the QuikChange Site-directed Mutagenesis Kit following the instructions of the manufacturer (Stratagene; La Jolla, CA) using the primers listed in Supplemental Table 1.
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bioRxiv - Neuroscience 2023Quote: ... were used for each replicate and placed into 100 µL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA quality was evaluated using the 5200 Fragment Analyzer System and the Agilent HS RNA Kit (Agilent Technologies, Inc., CA, USA). After the ribosomal RNA depletion with the RiboPOOL kit (siTOOLs Biotech GmbH ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Systems Biology 2023Quote: ... and confirmed the quality of the RNA samples using a Fragment Analyzer (Advanced Analytical Technologies, Inc.) with the Standard Sensitivity RNA Analysis Kit (Agilent, #DNF-471).
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bioRxiv - Neuroscience 2023Quote: ... Total RNA of 100ng was subjected to reverse transcription using the AffinityScript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A 2 µl aliquot of processed gDNA was taken to assess shearing using an Agilent High Sensitivity D1000 Kit (Agilent, Cat. #G2991AA). Once shearing was assessed ...
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bioRxiv - Molecular Biology 2023Quote: ... and the size distribution of the library was assessed using an Agilent 2100 Bioanalyzer with a High-Sensitivity DNA analysis kit (Agilent, 5067-4626). DETECTOR-seq libraries were sequenced on an Illumina HiSeqX platform to a depth of 10 million 150 bp paired-end reads per sample ...
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bioRxiv - Immunology 2023Quote: ... Pool quality control was performed with an Agilent High Sensitivity D1000 Screen Tape reagent kit using the Agilent 4200 TapeStation (Agilent Technologies, USA). Whole-genome sequencing (WGS ...
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bioRxiv - Cell Biology 2023Quote: pLV-CMV-IRIS-PURO-c-Src-mScarlet plasmid was used to the generation of Y527F and Y527F/K295R mutants via QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200523) according to manufacturer’s protocol using the primers listed in Table 1.
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bioRxiv - Cancer Biology 2023Quote: ... The labeling and hybridization steps were conducted in accordance with the provided instructions using the SureTag DNA Labelling kit (Agilent Technologies, USA). Equal amounts of DNA labeled with fluorescent markers were combined and co-hybridized onto a 44LK DNA microarray ...
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bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
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bioRxiv - Plant Biology 2024Quote: ... PCR probes were labelled with [α-32P]-dCTP (Hartmann Analytic #SRP-305) using the Prime-it II Random Primer Labelling Kit (Agilent, #EK0031) and purified on illustra Microspin G-50 columns (Cytiva #27533001 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from the beads and purified according to the manufacturer’s instructions using an Absolutely RNA nanoprep kit (Stratagene, La Jolla, CA) with in column DNA digestion and frozen prior to gene expression profiling being performed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the library profile was assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was then sequenced to approximately 50x coverage on an Illumina HiSeq 4000 instrument (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Quality and quantity of the finished libraries were assessed using an Agilent 4200 TapeStation DNA 1000 kit (Agilent Technologies, Santa Clara, CA) and Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ng of purified RNA was mixed with RNA standards (One Colour RNA Spike-In Kit; Agilent, Santa Clara, CA – 5188–5282) and the mixture labelled with LowInput QuickAmp Labeling Kit One-Color (Agilent 5190-2305) ...
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bioRxiv - Biochemistry 2024Quote: ... Introduction of mutation (ATG to TAA) into the pcDNA3.1-HA-mGluR2-mCitrine construct was performed with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Catalog no. 200523) (for primer pairs ...
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bioRxiv - Genomics 2024Quote: ... #Q32854) was used to assess RNA concentration and RIN values were determined using the Bioanalyzer Pico RNA kit (Agilent cat. #5067-1513), with average RIN scores of 8.2 for the adrenal gland ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA and library quality control were performed using an Agilent 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent #5067-4626). Sequencing was performed by Azenta Biosciences using a NovaSeq instrument.
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bioRxiv - Plant Biology 2024Quote: ... The quality of RNA was assessed using an Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Only samples with an RNA integrity number (RIN ...
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bioRxiv - Developmental Biology 2024Quote: Individual and combination mutant versions of Homo sapiens CTNNB1 DNA constructs were generated using standard cloning techniques and the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, 200521), unless otherwise noted ...
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bioRxiv - Physiology 2024Quote: ... Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by Qubit 2.0 fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA integrity and concentration was measured using an Agilent 2100 Bioanalyzer System with the RNA 6000 Pico Kit (Agilent Technologies, 5067-1513).
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bioRxiv - Neuroscience 2024Quote: ... quality and integrity of total RNA were assessed with an Agilent 2100 Bioanalyzer using an RNA 6000 Nano Kit (Agilent Technologies, Ltd.). For bulk RNA-seq strand-specific polyA-enriched RNA libraries were prepared using the KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... S59D and R120G+S59A mutations were introduced in pHR-CRYAB-mCh-Cry2 construct using QuikChange II site-directed mutagenesis kit (Agilent, CAT#200523). These constructs were subsequently cloned into pcDNA 3.1 mammalian vector for the expression in HEK293A cells ...
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bioRxiv - Systems Biology 2024Quote: ... Average dsDNA library size distribution was determined using the Agilent HS NGS Fragment (1-6000 bp) kit on the Agilent Fragment Analyzer (Agilent Technologies, USA). Libraries were normalized and pooled in the normalization buffer (10 mM Tris-Cl ...
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bioRxiv - Systems Biology 2024Quote: ... DNA molecular size was assessed with 5200 Fragment Analyzer System with the kit HS Genomic DNA 50 kb (DNF-468-0500) (Agilent Technologies, USA) and the software ProSize 3.0 (Agilent Technologies ...