Labshake search
Citations for Agilent :
6451 - 6500 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the final PCR step has been modified using the KAPA HiFi HotStart Ready Mix.The cDNA libraries were quantified using Fragment Analyzer 5300 (Advanced Analytical) kit FA-NGS-HS (Agilent, Cat#: DNF-474-1000) and Spectramax M2 (Molecular Devices ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Mitochondrial respiration was assessed by measuring the oxygen consumption rate (OCR) using the Seahorse XFe24 flux analyzer and XF Cell Mito Stress test kit (Agilent, Santa Clara, CA) and according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... BG505 Env mutants and additional JR-CSF Env mutants were generated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Santa Clara, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... The RNA quality was checked by Trinean Xpose (Trinean NV, Gentbrugge, Belgium) and Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). rRNA was removed from total RNA with Ribo-Zero rRNA Removal Kit (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... and the combination of antimycin A/rotenone (1 µM) into the culture medium (Seahorse XF Cell Mito Stress Test Kit, Agilent Technologies, 103015-100). Glutaminolysis was performed by using respiration kit (Seahorse XF Cell Mito Stress Test Kit ...
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bioRxiv - Cell Biology 2024Quote: ... T9A2-APOE4 same empty vector as reference was measured by using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, 103015-100) and Seahorse XFe96 FluxPak (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... The quality and composition of the extracted RNA were assessed using the Agilent 2100 Bioanalyzer system with the RNA 6000 Pico Kit (Agilent, Santa Clara, USA). Ribosomal RNA (rRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The integrity and quality of RNA were assessed on an Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies, CA, USA). Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Neuroscience 2022Quote: ... with the standard-sensitivity RNA chip or an Agilent 2100 Bioanalyzer system with the RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, USA) was used to analyze the extracted RNA samples ...
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA quality was assessed by determining RNA integrity numbers (RINs) using the Agilent 2100 Bioanalyzer system with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Immunology 2022Quote: ... for fragment size using the High Sensitivity NGS Fragment Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-474-0500; Agilent, Santa Clara, CA, USA), and functional validation using the Universal Library Quantification kit (catalog # 07960140001 ...
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bioRxiv - Immunology 2022Quote: ... and for integrity using the Standard Sensitivity RNA Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-471-0500; Agilent, Santa Clara, CA, USA). A total of 250ng total RNA was used for each sample as input to the TruSeq Stranded Total RNA library prep kit with unique-dual indexing (catalog #s 20020598 & 2002371 ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 0.3 μg of total RNA using the Affinityscript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, US). qPCR was performed using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA extraction was performed using a FinePure Plant RNA Kit (Genfine Biotech Co. Ltd., Beijing, China) and the qualities were assessed using a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA). A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity (RIN) was assessed using a Bioanalyzer 2100 instrument in conjunction with the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Genomics 2022Quote: ... RNA samples were then quantified and checked for RNA integrity using an Agilent BioAnalyzer 2100 with RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA), and only samples with an RNA integrity number ≥8 were sent for RNA-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... I15K/R54P) were generated by mutagenesis of pcDNA-3XFLAG-TRIM25 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA), while the TRIM25-PTAA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... The quality and quantity of the extracted RNA samples were analyzed with a 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA). Paired-end cDNA libraries were prepared from 0,2 ng of extracted RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Neuroscience 2022Quote: ... cDNAs were synthesized through reverse transcription from the total RNA according to the protocol of Affinity Script Multiple Temperature cDNA synthesis kit (Agilent Technologies, Santa Clara, CA). Expression levels of genes encoding plp (forward primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Cancer Biology 2020Quote: ... Target capture were performed on pools of 96 libraries using a Sure Select® user-defined probe kit (Agilent Technologies, Palo Alto, CA, USA). Massively parallel sequencing was carried out in a High-Seq® machine (Illumina ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid phapXVENUS-hph (Gsaller et al., 2014) was used as template and modified using QuikChange II site-directed mutagenesis kit (Agilent for K38E and R50A) or Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... The fragment distribution of each library was assessed the using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA High Sensitivity kit (ThermoScientific ...
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bioRxiv - Genomics 2021Quote: ... exome enrichment was applied to the cDNA purified from the previous step to enrich spliced cDNA by using probe kit SSELXT Human All Exon V8 (Agilent, catalog no. 5191-6879) and the compatible reagent kit SureSelectXT HSQ (Agilent ...
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bioRxiv - Immunology 2021Quote: cDNA was synthesized from the previously extracted RNA of mice colon tissue by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random 9mer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... after the molecular cloning the wt sequence was restored by mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, CA), the pETM11-CspA plasmid as DNA template and the mutagenic primers G670 5′-TTTCAGGGCGCCATGTCCGGTAAAATGACTG-3′ and G671 5′-CAGTCATTTTACCGGACATGGCGCCCTGAAA-3′.
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bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...
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bioRxiv - Genomics 2021Quote: ... HMW DNA was also imaged with a gDNA 165 kb kit (FP-1002-0275) on the Femto Pulse system (Agilent, Santa Clara, CA, USA). A detailed procedure is deposited in protocols.io (dx.doi.org/10.17504/protocols.io.bafmibk6).
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bioRxiv - Neuroscience 2020Quote: The α10W55T mutant subunit was produced as previously described (Boffi et al., 2017) using Quick change XL II kit (Stratagene, La Jolla, CA, USA). The chimeric α10x construct was generated in two steps ...
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bioRxiv - Genomics 2022Quote: ... Quantity were assessed on a Qubit 2.0 Fluorometer using a Qubit RNA HS Assay kit and the quality were checked by capillary electrophoresis on an Agilent Bioanalyzer using the RNA 6,000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA). Purified RNA was stored at −80 °C until further use.
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bioRxiv - Neuroscience 2022Quote: ... These constructs were made by performing site-directed mutagenesis on the Syt9 cDNA with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA). Full-length CLM constructs were used for the rescue experiments in Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... Genome-wide expression profiling was performed using the low-input QuickAmp labeling kit and human SurePrint G3 8×60K pangenomic microarrays (Agilent Technologies, Santa Clara, USA) as previously described (Merdrignac et al ...