Labshake search
Citations for Agilent :
6351 - 6400 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Purified RNA was then subjected to RNA integrity number (RIN) calculation using the Agilent RNA 6000 Pico kit (Agilent Technologies, Santa Clara, CA). RINs exceeding the Visium optimization threshold of 7.0 were confirmed for sections from all three blocks of interest.(40)
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bioRxiv - Immunology 2022Quote: ... a variant mutated to change the tyrosines at positions 179 and 181 to phenylalanines was generated using a QuikChange II Site-Directed Mutagenesis Kit (#200523; Agilent, Santa Clara, CA) and the primer 5’ CATCCCCGCCTTCGCCTTCTATGTCTCACGTTGG 3′ ...
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bioRxiv - Biochemistry 2022Quote: ... The pcDNA3.1 Flag-mRBPJ A284V CRr and the pcDNA3.1 Flag-mRBPJ F261A/A284V CRr were generated via site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies 200521-5) accordingly to manufacturer’s instructions with the oligos listed in Table S6 and using the pcDNA3.1 Flag-mRBPJ WT CRr and the pcDNA3.1 Flag-mRBPJ A284V CRr as templates ...
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bioRxiv - Developmental Biology 2022Quote: ... The RNA integrity was assessed using an Agilent 2100 Bioanalyzer using an RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA, USA). All samples had a 260:280 ratio > 2.0 and an RNA integrity number (RIN ...
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bioRxiv - Immunology 2022Quote: Specific amino acid changes on envelope clones and CAP206-CH12 heavy chain CDRH3 were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog #210519, Agilent Technologies, CA, USA). Mutations were confirmed by sequence analysis.
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Evolutionary Biology 2022Quote: ... samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Concentration of the libraries were determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Microbiology 2021Quote: ... The average sample fragment length and purity was determined using the Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). After passing quality control measures ...
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bioRxiv - Molecular Biology 2021Quote: ... was used to create fluorescent complementary RNA (cRNA) followed by hybridization to microarrays using Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, USA). Fluorescence signals were detected using SureScan Microarray Scanner (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were then loaded in equal volumes into 24 wells of an Agilent 3100 OFFGEL Fractionator using a high-resolution fractionation kit (Agilent Cat#5067-0201). Each sample ...
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bioRxiv - Immunology 2020Quote: ... The amplified total cDNAs were analyzed by an Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA) and sheared to 150bp on the Covaris S2 machine (Covaris ...
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bioRxiv - Immunology 2020Quote: ... Approximately 400 ng of amplified cDNA was used to generate the Illumina sequencing library using the Agilent SureSelectXT Target Enrichment Kit (Agilent, Santa Clara, CA) for Illumina Multiplex Sequencing by using enrichment probes designed for A/California/04/2009(H1N1 ...
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bioRxiv - Biophysics 2020Quote: ... A thrombin site was introduced between the 6xHis-tag and the coiled-coil using the QuikChange Lightning mutagenesis kit (Agilent, Santa Clara, USA). Sequences of the products were confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... RNA quality and quantity were determined using Agilent RNA6000 Nano kit by capillary electrophoresis (Agilent 2100 bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). cDNA was synthesized from 1 μg of total RNA using oligo-dT primer and Superscript III reverse transcriptase (RT ...
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bioRxiv - Microbiology 2022Quote: ... and H249A were generated from the parent pLVX Kernow C1/p6 ORF1AAAPG-Ha tag-AAAPG IRES zsGreen construct via QuikChange XL site-directed mutagenesis kit (Stratagene, La Jolla, CA). using primers listed in (Supplementary Table 1).
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bioRxiv - Genetics 2020Quote: ... the exonic regions and flanking splice junctions of the genome were captured using the Clinical Research Exome kit (Agilent Technologies, Santa Clara, CA). Massively parallel (NextGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The quality of the library was assessed using High Sensitivity D1000 kit on a 2200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA). Sequencing was performed using the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... The mutated variants of AopW1 were generated by inserting mutations in the corresponding primers in combination with the Quick Change Lightening Site Directed Mutagenesis kit (Agilent Technologies, SC, USA). For this purpose ...
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bioRxiv - Plant Biology 2020Quote: ... Site-directed mutagenesis to generate the different H3.1 point mutant constructs was performed using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ADA2b coding sequence was cloned into pETDuet-1 (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR of complementary DNA was performed using the DyNAmo HS SYBR Green kit (Thermo-Fisher, Cat # F410L) on an Mx3000P machine (Stratagene, La Jolla, CA) using the primers listed in Supplementary File 3 ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using mid output 150-bp paired-end (PE ...
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bioRxiv - Genomics 2021Quote: ... and the library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were sequenced on an Illumina HiSeq2500 instrument (Illumina ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Biochemistry 2021Quote: ... Single amino acid changes in the RBD (N501Y, E484K, K417N) and combinations thereof were introduced using QuikChange (XL Site-Directed Mutagenesis Kit, Agilent, Santa Clara, USA). Resulting expression plasmids were purified using Nucleobond Xtra maxi ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies, United States) according to the manufacturer’s instructions and the respective parental vector as template ...
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bioRxiv - Cancer Biology 2020Quote: ... The slides were thawed at room temperature for 15 minutes prior to immunohistochemistry staining using Envision+ Dual Link System-HRP (DAB+) kit (DAKO, Carpinteria, CA, USA). Following fixation in 95% ethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The average sample fragment length and purity was determined using the Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). After passing quality control measures ...
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bioRxiv - Biophysics 2021Quote: ... Six mutants of TDP-43 PLD were further generated by use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, United States) (16).
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bioRxiv - Cell Biology 2020Quote: ... while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat. 50671513, Agilent Technologies, Santa Clara, CA, USA) as well as RNA integrity (RIN) ...
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bioRxiv - Cell Biology 2021Quote: ... Coding regions and intron/exon boundaries were sequenced on the Novogen platform (agilent v6, HiSeqX,) after enrichment with Agilent kits (Agilent Technologies, Wokingham, UK). Exomes data were analysed using a bioinformatics pipeline developed in-house using two modules ...
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bioRxiv - Immunology 2021Quote: ... in order to modify its DAG discrimination capacity 17 by replacing Gly652 with Ala (G652A) using the Quick-Change XL site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the oligonucleotides described in Table 1 ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Immunology 2021Quote: ... cDNA amplification was carried out at 13 cycles of amplification for all samples and assayed for quality and concentration using a Bioanalyzer 2100 and High Sensitivity DNA Kit (Agilent, Santa Clara, CA).
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bioRxiv - Genetics 2020Quote: ... a natural NcoI restriction enzyme site located within intron 3 of the SPINK1 gene in the context of the previously constructed Mut expression vector was firstly eliminated by means of the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Les Ulis, France) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The average length of the indexed cDNA libraries was determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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bioRxiv - Genetics 2020Quote: ... Variants were introduced into the wild type constructions using the Quick-change II XL site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA). Presence of an abnormally spliced transcript associated with the decrease of the normal transcript was considered as “Impact on splicing” ...
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bioRxiv - Microbiology 2020Quote: ... libraries were checked for quality and size distribution using the 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, Santa Clara, CA, USA). Library quantitation was performed by Qubit using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of the RNA samples was confirmed using the Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent, Santa Clara, CA). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2022Quote: ... followed by quality check (QC) and fragment size distribution using a High Sensitivity Tape station Kit (Agilent 2200, #5067-5584 and #5067-5585). The library was sequenced using the HiSeq 4000 Illumina platform.
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bioRxiv - Immunology 2022Quote: ... reverse transcription was carried out with 500ng of RNA using an Affinity Script qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the libraries were checked for quality and size distribution using an Agilent 2100 Bioanalyzer and the High sensitivity DNA kit (Agilent, San Diego, USA). Quantitation of the library was performed by Qubit using the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified DNA library was analyzed using an Agilent 2100 bioanalyzer with an Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA) and a Qubit 2.0 with Qubit 1X dsDNA HS Assay Kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutations were introduced into ATP8B2 and ATP8B4 using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and/or the SLiCE cloning method (Zhang et al ...
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bioRxiv - Bioengineering 2023Quote: ... The RNA quality was evaluated using a 5200 Fragment Analyzer System and an Agilent HS RNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were constructed Using MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The total RNA quantity and purity were analyzed by using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). RNA-seq libraries were constructed and then sequenced using an Illumina Novaseq™ 6000 (LC-Bio Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
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bioRxiv - Systems Biology 2023Quote: ... quality check and quantification of total RNA was done using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 pico kit (Agilent Technologies, 5067-1513). Total RNA library preparation was done by introducing 500 ng total RNA into Illumina’s NEBNext Ultra II directional mRNA (UMI ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...