Labshake search
Citations for Agilent :
6051 - 6100 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The quality and quantity of libraries were determined using Fragment Analyzer by DNF-474 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) and QuantiFluor dsDNA System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Microbiology 2024Quote: ... To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified total RNA samples (100 ng) were labeled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labeling kit (One-color; Agilent Technologies). The Cy3-labeled cRNA samples were checked for amount (>825 ng ...
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bioRxiv - Developmental Biology 2024Quote: ... HySp5-2992-mBS4:Luc and HySp5-2992-mBS5:Luc constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Genomics 2024Quote: ... RNA samples were quantified and RNA integrity numbers (RIN) derived using the Bioanalyzer High Sensitivity RNA 6000 Pico kit (Agilent, UK).
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bioRxiv - Genomics 2024Quote: ... and the RNA integrity number (RIN) was measured using a tape-station system with an RNA screen-tape analysis kit (Agilent Technologies).
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bioRxiv - Microbiology 2024Quote: ... The size distribution of the libraries was checked by high-resolution gel electrophoresis with a Bioanalyzer instrument using the DNA 7500 Pico kit (Agilent Technologies). Libraries were quantified through fluorometry with a QubitTM fluorometer and dsDNA HS reagents (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA libraries were constructed using the TruSeq Nano DNA kit and quality-checked in the TapeStation D1000 ScreenTape System (Agilent Technologies). Genomic libraries were sequenced on an Illumina NovaSeq with PE150 (paired-end ...
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bioRxiv - Plant Biology 2024Quote: ... thaliana cpSRP54 residues 1-564 including a point mutation at position 185 (At-cpSRP54(Q185R)) was generated using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies) using pCBi18-At-cpSRP54 as template ...
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bioRxiv - Developmental Biology 2024Quote: ... GHTF assessed the quality and quantity of the amplified cDNA by electrophoresis using Agilent’s Agilent High Sensitivity DNA Kit (Agilent: 5067-4626) and Bioanalyzer before library construction ...
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bioRxiv - Pathology 2024Quote: ... and the integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). 1 µg of total RNA was reverse transcribed in a 20 µL μnal reaction volume using the High-Capacity Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Biophysics 2024Quote: OCR measurements were performed with the Agilent Seahorse XF Cell Mito Stress Test kit with an XF24 extracellular flux analyser (Seahorse Bioscience). XF24 plates were coated with either 23 µl of 100% Matrigel (soft ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Genetics 2024Quote: ... was used for all experiments with integrated Cas9 (Supplementary Table 3).25 Cas9D10A was generated by site-directed mutagenesis of pLS504 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent #200521) using primers MK11 and MK12 ...
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bioRxiv - Cell Biology 2024Quote: The aforementioned mutations in the hypervariable regions of RhoA-GFP and RhoB-GFP were generated according to the manufacturing instructions of the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) using the mutagenic primers listed in the Key Resources Table ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521, Agilent Technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... The integrity and quantity of the total RNA was measured using an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... The INF2-ER[K792A] GFP construct was generated via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). All constructs were sequenced completely across their coding region.
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified with Agencourt AMPure XP beads and sample profile was analysed with the High Sensitivity D5000 kit (Agilent Technologies) on a TapeStation (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... The plasmid for expressing FLAG-Cas8bM545A was generated by performing a mutagenesis PCR using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) with the oligonucleotides 8M545A and 8M545Ar and pTA927-cas8b-FLAGC as template ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 400 ng of each RNA extraction was reverse transcribed with the 1st Strand cDNA synthesis Kit (Agilent Technologies) to obtain cDNA ...
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bioRxiv - Microbiology 2024Quote: ... The GTD mutants were introduced into pET28a-GTD using the Quick Change Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, USA) with the primers listed in Table S2 ...
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bioRxiv - Genomics 2024Quote: ... A small aliquot of libraries were amplified using the PacBio Onso library amp kit (PN: 102-410-800) for library QC purposes (library size measurement by Agilent TapeStation). Quantitative PCR was performed with the PacBio Onso library quant kit (PN ...
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bioRxiv - Microbiology 2024Quote: ... Site-directed mutagenesis was carried out using QuikChange site-directed mutagenesis kit as per the manufacturer instructions (Agilent Technologies, CA, US) and all the mutations were confirmed by sequencing (Eurofins Pvt ...
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bioRxiv - Immunology 2024Quote: ... dissolved in nuclease-free water and RNA integrity analyzed using a 2100 Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies, Waldbronn, Germany). Samples with DV200 values >30% were included for gene expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed using 5 μl of mRNA using the Ultra II Directional RNA Library Preparation Kit (Agilent, Santa Clara. CA). Indexes were designed according to the kit specifications and obtained from Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... with anti-mouse epitopes visualised with DAB chromogen in brown and anti-rabbit epitopes visualised with alkaline phosphatase in magenta or the Envision kit (mouse/rabbit) (K500711-2, Agilent, UK). Sections were counterstained with haematoxylin or methyl green and coverslipped with permanent mounting medium before imaging.
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bioRxiv - Microbiology 2024Quote: RNA quality control was performed with Agilent 4200 Tape Station system using RNA ScreenTape analysis kit (Agilent, Santa Clara, CA, USA). Only RNAs having RNA Integrity Number (RIN)>8 were used for library preparation ...
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bioRxiv - Immunology 2024Quote: ... The libraries were assessed for quality and quantity on a bioanalyzer using the DNA High Sensitivity Kit reagents and protocol (Agilent Technologies). The quantified libraries were pooled and templated and enriched on the Ion Proton One Touch system using the Ion PI™ Hi-Q™ OT2 200 Kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The size distribution of cDNA in each well as well as the concentration of cDNA in the 500 to 5000 bp range was subsequently determined using a High Sensitivity D5000 ScreenTape kit (Agilent Technologies) and readout analysed on an Agilent 4200 TapeStation System ...
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bioRxiv - Microbiology 2024Quote: ... Inactivating mutations of the HP1α-interacting chromatin protein mimicry in JC virus Agnoprotein were introduced using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (#200521) and the appropriate primers (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cyanine 3 (Cy3)-labeled complementary RNA was synthesized from 50 ng of total RNA using Low Input Quick Amp WT Labeling Kit (Agilent Technologies) and subjected to microarray hybridization ...
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bioRxiv - Genomics 2024Quote: ... and the size distribution of the DNA was estimated using the Femto Pulse system (Genomic DNA 165 kb kit, Agilent, CA), where 70% of the DNA fragments were found to be 30 kb or longer.
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bioRxiv - Microbiology 2024Quote: ... Each pool was quantified using qPCR (KapaBiosystems, #KK4854) and sized using the Agilent Bioanalyzer high-sensitivity DNA kit (Agilent, #5067-4642). The Illumina MiSeq platform with the MiSeq Reagent 222 kit v2 (#MS-102-2003 ...
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bioRxiv - Molecular Biology 2024Quote: ... The MRAP2 variants were introduced into a MRAP2-3xFLAG plasmid by site-directed mutagenesis using the Quikchange Lightning Kit (Agilent Technologies) and oligonucleotides from Sigma ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified RNA samples were analyzed for quality control using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano kit (Agilent Technologies). Microarray experiments were performed using a custom-designed Agilent 8 × 60 K array for the E ...
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bioRxiv - Developmental Biology 2024Quote: ... The quality and concentration of the libraries were assessed using the Agilent High Sensitivity DNA Kit and Bioanalyzer 2100 (Agilent Technologies) and submitted to 150 bp paired-end high throughput sequencing using Hiseq4000 (Illumina).
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
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bioRxiv - Microbiology 2024Quote: ... in which the glutamic acid in the active site was substituted by alanine employing QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) and appropriate primers listed in SI appendix ...
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bioRxiv - Zoology 2024Quote: ... RNA integrity was verified using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RIN values ≥ 7.0 were considered appropriate for mRNA library preparation ...
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bioRxiv - Immunology 2024Quote: The integrity of the isolated RNA (according to the aforementioned protocol) was measured with a Fragment Analyzer using RNA Kit 15 nt (Agilent Technologies). Samples with an RNA integrity number ≥ 8 were used for sequencing ...
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bioRxiv - Immunology 2024Quote: ... Detection was achieved using Leica DAB Detection Kit (Bond Polymer Refine Detection) for CD4 and Envision FLEX DAB+ (Diaminobenzidine) (Dako Omnis) for BCL6 ...
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bioRxiv - Microbiology 2024Quote: ... Site-directed mutagenesis of the Trp252 residue in BoGH5A was performed using the QuikChange II Site Directed Mutagenesis (SDM) Kit (Agilent Technologies) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Molecular Biology 2024Quote: The ATG (+1) codon of pKL-RLBP1 was mutated to an alanine codon (GCG) using the QuikChange Site-directed Mutagenesis kit (Agilent technologies) with the F primer and its reverse complement (Table S1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The amount and quality of RNA was measured with the Agilent RNA 6000 Nano Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies), according to the manufacturer’s instructions ...
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Development of ketobenzothiazole-based peptidomimetic TMPRSS13 inhibitors with low nanomolar potencybioRxiv - Pharmacology and Toxicology 2024Quote: ... R560 and K561 and addition of a 6xHis tag at the c-terminal using the QuikChange Lightning site-directed mutagenesis kit (Agilent, 210518).
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bioRxiv - Physiology 2024Quote: ... The libraries were analyzed for size distribution and concentration using the BioAnalyzer High Sensitivity DNA kit (Agilent technologies, Santa Clara, CA). Libraries were pooled at equimolar concentrations and sequenced on a Novaseq6000 (Illumina ...