Labshake search
Citations for Agilent :
5951 - 6000 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... on a Qubit Fluorometer (invitrogen) and fragment length was assessed with an Agilent 2100 Bioanalyzer (Agilent High sensitivity DNA Kit, Agilent). Samples were equimolarly pooled and sequenced on NextSeq™ 500 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Cell Biology 2024Quote: Mutations in the DFCP1 (K193A and R266Q) and ATGL (D166G) were introduced using the QuikChange mutagenesis kit (Agilent, Santa Clara, CA).
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bioRxiv - Bioengineering 2024Quote: ... and extracellular acidification rate (ECAR) were measured following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of each library was analyzed on the Bioanalyzer with a High Sensitivity DNA analysis Kit (Agilent technologies) and Collibri Library Quantification Kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of each library was analyzed on the Bioanalyzer with a High Sensitivity DNA analysis Kit (Agilent technologies) and Collibri Library Quantification Kit (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... The fragment was gel purified and used as template in error-prone PCR (EP-PCR) using the GeneMorph II kit and Mutazyme II polymerase (Agilent Technologies) and the primers (5RT 3RT) ...
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Developmental Biology 2024Quote: ... Post cDNA amplification and post-library construction quality control was performed using the Agilent Bioanalyzer High Sensitivity kit (Agilent 5067–4626). Libraries were sequenced using a NovaSeq 6000 and the S2 flow cell ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was fragmented into 180–280 bp by sonication and subjected to library preparation using the Agilent SureSelect Human All Exon V6 Kit (#5190-8864, Agilent Technologies). The enriched libraries underwent paired-end 150bp sequencing on the Illumina HiSeq 2000 platform.
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bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial probes used for visualization of mt-mRNA and mt-rRNA levels were restriction fragments labeled with α-32P-dCTP and a random priming kit (Agilent). Different mitochondrial tRNAs and 7S RNA were detected using specific oligonucleotides labeled with ψ-32P-ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were analyzed on Fragment Analyzer with Genomic DNA 50kb Kit and TapeStation with either High Sensitivity D5000 ScreenTape System or High Sensitivity RNA ScreenTape System (Agilent Technologies) according to the manufacturer’s protocols [41-43] ...
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bioRxiv - Neuroscience 2024Quote: ... and assessed for RNA integrity using an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). The sequencing libraries were then multiplexed and loaded onto the Illumina NovaSeq 6000 instrument’s flowcell ...
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bioRxiv - Immunology 2024Quote: ... we first inserted the SalI site at just downstream of the termination codon of the dsRed cDNA of pIRES2-dsRed-IRF418 using a QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies), and isolated the BglII-SalI fragment containing the mIRF4-IRES-dsRed DNA region from the mutated plasmid ...
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bioRxiv - Genomics 2024Quote: ... quality control was performed by run on an Agilent 2100 Bioanalyzer System using the High Sensitivity DNA Kit (Agilent, 5067-4626). Libraries were then created from each fraction using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The quality of the RNA samples was assessed using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Kit, 5067-1511, Agilent technologies) following manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... DNA quality and size were assessed using an Agilent 4200 TapeStation with a gDNA ScreenTape Kit (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells under the indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia) and measured using an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 libraries were generated from murine tissue samples and libraries were quantified using the Agilent DNA 1000 kit (Agilent Technologies, USA).
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bioRxiv - Immunology 2023Quote: ... The predicted CLIPA8 cleavage site 63IMLR66 was replaced by IEGR [26] using the CLIPA8mutag primer and the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to create plasmid pOET3-CLIPA8Xa-V5-His (Table S3) ...
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bioRxiv - Biochemistry 2023Quote: ... was used as a template to generate deletion variants for expression in insect cells using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). For bacterial expression of the ΔN790 variant ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control for the constructed library was performed by the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies; Catalog # 5067-4626), while Qubit DNA HS Assay Kit was used for qualitative and quantitative analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification and quality checked of libraries was done using an Agilent 2100 Bioanalyzer instrument and a DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 S1 100 cycle run (v1.5 SBS reagents) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The quality of each library was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent 5067-4626). The libraries were submitted for sequencing at Cancer Research UK Cambridge Institute Genomic Core Facility using Illumina NovaSeq with 100bp single-end reads.
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bioRxiv - Cell Biology 2023Quote: The NGS sample was diluted with TE buffer and assayed using a High Sensitivity DNA Kit (cat. #5067-4626, Agilent Technologies) on a Bioanalyzer 2100 ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Genetics 2023Quote: ... RNA quantity and quality were assessed using NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies) and Agilent 2100 Bioanalyser (RNA 6000 Nano LabChip kit, Agilent Technologies).
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: Seahorse experiments were performed on sorted CD8+ cells activated in presence or absence of LSDi using XF Cell Mito Stress kit (Seahorse Bioscience). OCR and ECAR were measured with XF96 Extracellular Flux Analyzers (Seahorse Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was quantified and quality checked using Bioanalyzer 2100 instrument in combination with RNA 6000 nano kit (both Agilent Technologies). Libraries were prepared from 800 ng of input material (total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The above residue mutants were created by site-directed mutagenesis following instructions provided in the QuickChange XL Site-Directed Mutagenesis Kit manual (Agilent Technologies). All other full-length UNK residue mutants ...
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bioRxiv - Microbiology 2023Quote: ... into AAATA in the DENV-2 wild type plasmid (pFK-DVs) was generated using QuickChange XL site-directed mutagenesis kit (Agilent, 200517) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA integrity was determined using capillary chip-based electrophoresis with an RNA 6000 Nano Methods 59 LabChip Kit and an Agilent Bioanalyzer 2100 (Agilent, USA) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... GGC -> GGT) of dCas9 on pKR387 to remove an AscI recognition site using the QuikChange II site-directed mutagenesis kit (Agilent #200523). The resulting product was transformed into NEB 10-beta cells using a standard heat shock protocol and transformants were selected on LB agar plates with 100 μg/mL of carbenicillin ...
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bioRxiv - Neuroscience 2024Quote: RNA from sorted cells were analyzed on the Agilent 2100 Bioanalyser system using the Eukaryote Total RNA Pico kit (Agilent Technologies) and the mean RIN number was 9.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Twin-strep-Flag-HALO-DVL3 or pCS2+xDvl3 vector was performed using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following a manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of RNA samples was used for reverse transcription with the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent; 200820) using random hexamer primers ...
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bioRxiv - Microbiology 2023Quote: ... The purified libraries were then assessed for their fragment sizes using the High Sensitivity DNA kit on the Bioanalyzer 2100 (Agilent Technologies). After applying the NetoVIR protocol ...
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bioRxiv - Immunology 2023Quote: ... The size of the resulting libraries was determined with the Agilent D1000 reagent kit and Agilent 4200 TapeStation (Agilent Technologies, USA). Pooling was performed automatically using a Tecan Freedom EVO robotic station (Tecan ...
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bioRxiv - Immunology 2023Quote: ... and quality was verified on an Agilent 2100 Bioanalyzer instrument with the Agilent DNA 1000 kit (Agilent Technologies, Catalog# 5067-1504). Additionally ...
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bioRxiv - Molecular Biology 2023Quote: ... The desired PNUTS mutant in the RVXF motif (converting the RISW motif to RASA) was generated by oligonucleotide-mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell RNA-seq and CMO libraries were prepared according to the manufacturer’s protocol and the library quality was confirmed with a Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). Cellecta barcode libraries were prepared using 25% of the adaptor-ligated cDNA to amplify only the barcoded cDNA product ...
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bioRxiv - Biochemistry 2023Quote: Mutations were generated by site-directed mutagenesis (table 2) on this plasmid using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and plasmids were verified through sequencing through the Molecular Informatics Core at the University of Rhode Island.
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bioRxiv - Biochemistry 2023Quote: ... The desired PP1 or PNUTS mutants were generated by oligonucleotide- mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... H80S/D83S and G93A mutants were generated by the use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) (60) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... as previously described.19 Site-directed mutagenesis was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Protease activity of NIa-Pro was abolished by mutating cysteine 151 to alanine (C151A) using the same construct and the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA) in both pMDC32 and pSITE (Huogen et al. ...
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bioRxiv - Biophysics 2023Quote: ... site-directed mutagenesis was used using the vector pET21a-αSynuclein with the kit QuikChange XL Site-Directed Mutagenesis (Agilent technologies, USA) and the inserted mutations were checked by sequencing ...
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bioRxiv - Immunology 2023Quote: ... RNA quality was verified using the Fragment Analyzer (Advanced Analytical) and the RNA kit (DNF-471, Agilent Technologies, Wilmington, DE, USA). All samples with an RNA Quality Number (RQN ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-specific mutagenesis was performed according to standard procedures of the QuickChange Site-Directed Mutagenesis Kit purchased from Stratagene (GE Healthcare) or Mut Express II (Vazyme Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... Quality control checks and quantification were performed using an Agilent 2100 Electrophoresis Bioanalyzer instrument with the Agilent RNA 6000 Pico kit (Agilent Technologies), and a Qubit 2.0 fluorometer with the Qubit RNA HS Assay kit (Thermo Fisher Scientific) ...