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Citations for Agilent :
5851 - 5900 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... serial sections were stained singly with antibodies against BCL6 and CD8 and a DAB DAB visualization kit (Envision Double Stain system, Dako; USA) for bright field microscopy ...
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bioRxiv - Systems Biology 2020Quote: ... Immunohistochemical staining was obtained performed using a biotinylated-streptovidin-peroxidase complex (Dako Universal LSAB™+ Kit/HRP-K0690, Dako, Glostrup, Denmark) with DAB (3,3-Diaminobenzidin)-chromogen (Dako-K3468 ...
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bioRxiv - Molecular Biology 2021Quote: ... Site-specific mutation of K674R was introduced in pQCXIP-FLAG-MD by using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The quality of the remaining RNA was checked using a Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA) (all RIN values > 9 ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Biochemistry 2021Quote: G4-LMCA1: Four glycines between residue K44 and D45 in LMCA1-pET-22b were introduced using the QuickChange mutagenesis kit (Agilent Technologies) and verified by Sanger sequencing (Eurofins ...
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bioRxiv - Systems Biology 2020Quote: ... Final SMRTbell libraries were quantified using Qubit and qualitative and quantitative analyses were performed using BioAnalyzer DNA 12000 kit (Agilent Technologies), and Fragment Analyzer Large-Fragment kit (Agilent Technologies) ...
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bioRxiv - Plant Biology 2020Quote: The K1370R point mutation was introduced into the binary construct pTOC159-GFP-TOC159GM using a site directed mutagenesis kit (Agilent-QuikChangeII) with the primers TOC159S3F and TOC159S3R and resulted in pTOC159-GFP-TOC159GM-K/R ...
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bioRxiv - Plant Biology 2020Quote: ... The point mutation was introduced in the binary construct 35S-GFP-TOC159GM by using a site-directed mutagenesis kit (Agilent-QuikChangeII) with the primers TOC159S3F and TOC159S3R ...
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bioRxiv - Biochemistry 2021Quote: The removal of the C327-T331 segment from the CM domain of PniDAH7PS was performed using a QuikChange® II Site-Directed Mutagenesis Kit (Stratagene). The pET28a-PniDAH7PS plasmid was used as the template ...
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bioRxiv - Genomics 2021Quote: ... Quality check and quantification of total RNA were performed using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 Nano Kit (Agilent Technologies). Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Molecular Biology 2020Quote: ... p.C131S active site dominant negative (DN) missense mutation was introduced into pCMV-UBE2E1-FLAG-cmyc using the QuickChange mutagenesis kit (Stratagene Inc.) and verified by DNA sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... All additional site-directed variants of GtCPES and PmCpeB were generated from pGtCPES or pCOLACpeB using the QuikChange® site-directed mutagenesis kit (Stratagene) with help of primers listed in Table 4 (only the forward primer is shown ...
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bioRxiv - Genomics 2021Quote: ... RT qPCR analysis was run for the samples using a miScript SYBR Green PCR Kit (Cat. No. 218073) on a Stratagene Mx3005P (Agilent Technologies). Housekeeping gene GAPDH primers were purchased from Qiagen (Cat ...
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bioRxiv - Genetics 2021Quote: RNA-sequencing libraries were prepared using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing kit (Agilent, Cat#: G9691A) according to the manufacturer’s protocol using 100 ng total RNA ...
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bioRxiv - Cell Biology 2021Quote: ... The mutant eGFP-CCR5-L196K (substitution of L196 with a lysine) was generated by site-directed mutagenesis using the QuickChange II Mutageneis kit (Agilent Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Disruption of the Lmxb1 binding sites was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) following manufacturer recommendations and confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2020Quote: ... HA-USP29 siRNA-resistant and the catalytically inactive USP29C294S were generated using the QuikChange® II XL Site-Directed Mutagenesis Kit (Stratagene) and the oligos reported in Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... amino acid substitutions were introduced using a QuikChange II XL site directed mutagenesis kit (200521) purchased from Agilent (Santa Clara, CA). After transformation into BL21-Gold (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The quality of obtained libraries were analyzed using Agilent Bioanalyzer 2100 and High Sensitivity DNA kit (Agilent, cat. no. 5067-4626). Finally ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... Total RNA from all other cell lines was used to prepare cDNA sequencing libraries using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies) after the polyA selection step ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Biochemistry 2021Quote: ... Multi-site-directed mutagenesis was carried out using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... The quality and quantity of RNA was determined by capillary electrophoresis using HS RNA kit and 5200 Fragment Analyzer (Agilent Technologies). For single-end RNA sequencing (RNA-seq) ...
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bioRxiv - Genomics 2021Quote: ... site-directed mutagenesis was performed targeting desired codon positions of the rplD and rplV genes on the pS10 plasmid [20] using the QuikChange Lightning kit (Agilent Technologies) and the primers indicated in Supplemental Table 2 ...
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bioRxiv - Biophysics 2021Quote: ... the double-mutant S11N/G74S variant and the chimera involving the loop [70-77] were introduced using the QuikChange Lighting Site-Directed Mutagenesis kit (Agilent Technologies) and the sequences were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... followed by library interrogation using a Bioanalyzer 2100 instrument with Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc. Part# 5067-4626).
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Neuroscience 2021Quote: ... Cy3- or Cy5- labeled cRNA probe was synthesized from 150 ng of total RNA using the Quick-Amp Labeling Kit (Agilent Technologies). Quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-2000 spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... cDNA before and after TCR enrichment was profiled using both Qubit (Thermofischer scientific) and Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). Libraries for RNA-seq and V(D)J were prepared following the manufacturer’s user guide (10X Genomics) ...
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bioRxiv - Molecular Biology 2021Quote: ... Enzymatically-dead zDHHC3 mutant transgenic mice overexpressing zDHHC3DHHS in cardiomyocytes were generated by site-directed mutagenesis of the α-MHC-Zdhhc3 promoter-transgene construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) to encode a mutation of Cys-157 in mouse zDHHC3 protein to Ser ...
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bioRxiv - Molecular Biology 2020Quote: ... integrity and the amount of total RNA were measured with a ‘Bioanalyzer 2100’ using an ‘RNA Nano 6000 Microfluidics Kit’ (both Agilent genomics). RNA labeling was performed with the two color ‘Quick Amp Labeling Kit’ (Agilent genomics ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Microbiology 2021Quote: ... The MPER changes (L669S, W672A, I675A, T676A and L679A) were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). All the mutations were confirmed by DNA sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA extracted from FACS isolated cells was quantified and assessed for quality using the RNA 6000 Pico Kit (Agilent – 5067-1513) and an Agilent 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2020Quote: ... Post cDNA amplification and post library construction quality control was performed using the Agilent Bioanalyzer High Sensitivity kit (Agilent 5067-4626). Libraries were sequenced using a NovaSeq 6000 and the S2 flow cell ...
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bioRxiv - Cell Biology 2021Quote: ... and -ΔPID (aa 524-571) domain deletions were generated by PCR and the R57D mutant by the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent, 5067-5582, 5067-5583, 5067-5586). Paired end sequencing was done using a HiSeq 2500 (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA was extracted from three replicates for each of the cell-lines followed by a quality assessment using Agilent Bioanalyser-2100 with Agilent nano Kit (Agilent Technologies). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies). Four hundred ng of total RNA yielded on average 12 μg of ds-cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... and a p72Plk3 point mutant (Plk3R354G) was generated via site-directed mutagenesis using a QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). All mutants were subcloned into the pCMV-Tag2A vector ...
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bioRxiv - Cell Biology 2022Quote: ... ASNA-1A63V::GFP was generated by mutagenesis of pVB222GK (Kao et al., 2007) to introduce the A63V change using the QuickChange II Kit (Agilent Technologies). The resulting plasmid (pGK200 ...
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bioRxiv - Microbiology 2022Quote: ... ΔNef ANCH3 (HIV-1 ANCH3 IND116A) was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent #200522), for integrase (IN ...
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bioRxiv - Molecular Biology 2022Quote: ... The quality of the prepared libraries was assessed using an Agilent 2100 bioanalyzer and a High Sensitivity DNA kit (Agilent Technologies), or Fragment Analyzer and dsDNA 915 Reagent Kit (Agilent Technologies) ...