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Citations for Polysciences :
651 - 700 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 × 106 cells ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 24 hours at 37°C before the cells were transfected with 2 µg cDNA coding for hASIC1a (inserted into pcDNA3.1+ vector upstream IRES GFP) using Polyethylenimine (PEI) 25K (Polysciences) in a 1:3 ratio ...
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bioRxiv - Biochemistry 2024Quote: Plasmid transfections were performed using polyethylenimine (PEI, Polysciences 23966-1) at a 1:6 ratio of DNA (in μg ...
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bioRxiv - Cell Biology 2024Quote: ... was co-transfected with packaging plasmids pMD2.G (500 ng) and psPAX (500 ng) into 293T cells using 12 μl PEI (Polysciences) in a 6-well plate for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid DNA and polyethyleneimine max (PEI max, Polysciences) were separately diluted to 1μg/mL and 0.05 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... U2OS and HeLa229 cells were transfected using the commercially available transfection reagents jetOptimus® (Polyplus®, Illkirch- Graffenstaden, France) or PEI MAX® (Polysciences, Inc., Warrington, United Kingdom) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μL of 1 mg/mL polyethylenimine (PEI MAX®, Polysciences 24765) and 200ng of DNA of pSems leader ALFAtag-γc and 2800ng of pSems leader mXFPe1-IL-2Rβ79 ...
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bioRxiv - Biochemistry 2024Quote: ... 4 μL of 1mg/mL polyethylenimine HCl MAX Linear Mw 40k (PEI, Polysciences) in 25 mM K-HEPES pH 7.5 was applied to the grids ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of plasmids were pre-incubated with 2 mg of 40-kDa linear polyethylenimine (PEI) (Polysciences) in 25 ml of Opti-MEM™ (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and 800 ug Polyethylenimine (PEI 25K, Polysciences CatNo 23966-1), also diluted in 13 ml of Opti-MEM media ...
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bioRxiv - Biochemistry 2024Quote: ... Linear PEI MAX (MW 40,000) was from Polysciences, Inc ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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bioRxiv - Cell Biology 2024Quote: ... using PEI (Polyethylenimine, Polysciences) (Supporting Information Table S4) ...
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bioRxiv - Biochemistry 2024Quote: ... using polyethylenimine (Polysciences). After 6 days ...
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bioRxiv - Biochemistry 2024Quote: ... PEI (Polysciences) (1mg/mL stock ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid DNA via polyethylenimine (Polysciences). After 96 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection of overexpression- and promoter-deletion-constructs was performed using polyethylenimine (PEI) (Polysciences, 23966). For crotonate supplementation ...
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bioRxiv - Cancer Biology 2024Quote: ... using polyethylenimine (Polysciences 23966-1). Media was replaced the following day ...
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bioRxiv - Cancer Biology 2024Quote: ... and then transfected using PEI (Polysciences 49553-93-7) into HEK293T cells with packaging plasmids psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2024Quote: ... patient tissues or GBOs were fixed with 4% methanol-free formaldehyde (Polysciences) in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... polyethylenimine (PEI) (Polysciences, 23966-2), nicotinamide-free DMEM (US biologicals ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with Polyethylenimine (PEI) (Polysciences, Inc.) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... using PEI (Polyethyleneimine, 23966-100, Polysciences). 16 h post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mg/ml Polyethylenimine (PEI 25K, Polysciences) with Opti-MEM (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were coverslipped with Aqua-Poly/Mount mountant (Polysciences Inc cat#18606-20). Primary antibodies included ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then fixed with a solution containing 0.2% glutaraldehyde (Polysciences, 01909-100) and 2% formaldehyde (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... in the presence of 1 mg/mL polyethylenimine reagent (PEI) (Polysciences). To generate TP53-knockdown cell lines ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK 293T cells were transfected with 8 µg expression vectors and 2 µg of third-generation lentiviral packaging system in complex with 24µg PEI (Polysciences) in 1.2 ml of 0.9% NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were mounted on a slide (Aqua-Polymount, Polysciences).
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bioRxiv - Developmental Biology 2024Quote: ... Masson’s Trichome staining was performed per manufacturer instructions using the Masson’s Trichome Stain Kit (Polysciences cat #25088).
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bioRxiv - Developmental Biology 2024Quote: Guts from adult female flies were dissected in PBS and immediately fixed in 4% formaldehyde (FA, EM grade, Polysciences) diluted in PBS for 1h at room temperature (RT ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were sorted and mounted in Aqua-PolyMount (Polysciences).
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bioRxiv - Developmental Biology 2024Quote: ... A second droplet of (∼ 10 µl) of a solution of 1 µm polystyrene microspheres (Polysciences, cat. no. 07310-15) was then added and gently mixed ...
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bioRxiv - Developmental Biology 2024Quote: ... tissues were embedded using a Poly/Bed 812 kit (Polysciences) and polymerized in an electron microscope oven (TD-700 ...
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bioRxiv - Developmental Biology 2024Quote: ... the Masson’s Trichrome (MT) assay was performed according to the manufacturer’s instructions (Polysciences, Inc., Warrington, PA, USA) on three testicular sections from each male ...
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Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgutbioRxiv - Developmental Biology 2024Quote: ... The entire gastrointestinal tract was taken and fixed in 1 X PBS plus 8% EM grade formaldehyde (Polysciences) for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... a volume of Concanavalin A beads (Polysciences, 86057-3) corresponding to 15 µl per histone mark target was prepared by removing the supernatant on a magnetic rack and washing in 1 ml binding buffer (20 mM HEPES [pH 7.5] ...
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bioRxiv - Cell Biology 2024Quote: Worms were anesthetized in 0.1 mg/ml tetramisole in 1X M9 buffer on empty NGM plates and mounted on thin 2% agarose pads on glass slides with 0.05 mm Polybead microspheres (Polysciences) for immobilization ...
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bioRxiv - Developmental Biology 2024Quote: ... guts were fixed in situ for 25-30 min at room temperature in 8% formaldehyde (Polysciences 18814-20), 200 mM sodium cacodylate ...
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bioRxiv - Developmental Biology 2024Quote: ... BioMag Plus Concanavalin A beads (ConA beads, PolySciences NC1358578) were washed twice with cold bead activation buffer (20mM HEPES pH7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 3 µL 0.1 µm Polybead microsphere suspension (Polysciences) was added to the pad ...
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bioRxiv - Cell Biology 2024Quote: ... 15μg of the MigR1-OsTIR2-TagBFP construct and 15μg of pCL-Eco packaging plasmid was co-transfected into HEK293T cells using PEI (Cat#23966, Polysciences). 48 and 72 hours later ...
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bioRxiv - Cell Biology 2024Quote: ... and 48 μL of Polyethylenimine Max (Polysciences, 24765-2). Media was changed after 16 hr ...
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bioRxiv - Cell Biology 2024Quote: ... in amount ratio 1:1) per 1x106 cells was mixed with a threefold (w/w) of PEI (polyethyleneimine, linear MW 25000, Polysciences) in Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... the pFX-Lifeact-EGFP expression vector was transfected into MDCK cells using Polyethylenimine Max (Polysciences, Warrington, PA, USA). To establish cell lines stably expressing CLCa-EGFP ...
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bioRxiv - Cell Biology 2024Quote: ... For the production, third-generation packaging, and envelope vectors (pMDL, psRev, and pMD2G) were used in conjunction with Polyethyleneimine (PEI Polysciences PN 23966) in DPBS (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus was produced by co-transfecting Cas9-T2A-BFP-Flag plasmid along with two viral packaging plasmids into HEK293 FT cells using Polyethylenimine transfection reagent (PolySciences, 9002-98-6). Approximately 48 hours after transfection ...
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bioRxiv - Genetics 2024Quote: ... Coverslips were mounted on slides using Aqua-Poly/Mount (Polysciences, Inc.). A Zeiss LSM700 microscope equipped with 40x Plan-apochromat 1.3 NA oil immersion lens (Carl Zeiss Micro Imaging Inc. ...
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bioRxiv - Genetics 2024Quote: ... samples were mounted with Aqua Poly/Mount (Polysciences) and observed under a fluorescent microscope to determine the percentage of phagocytic cells ...
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bioRxiv - Cell Biology 2024Quote: 50µL of Fluoresbrite YG carboxylated microspheres (1μm, catalogue#: 15702-10, Polysciences) diluted in pericyte medium was spiked into pericyte cell medium in 24-well plates ...