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Citations for Polysciences :
2951 - 3000 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... we positioned borosilicate microspheres (diameter < 10µm, Polysciences) at the ends of tipless AFM cantilevers (Arrow TL2 ...
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bioRxiv - Microbiology 2020Quote: ... 500 nm (18720, Polysciences) or 970 nm (PMC 1N ...
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bioRxiv - Cell Biology 2020Quote: ... snap frozen and embedded in Polyfreeze medium (Polysciences). Sagittal sections (∼ 16 µm thick ...
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bioRxiv - Cell Biology 2020Quote: ... and cell pellets resuspended in 9.37 ml PBS plus 0.63 ml 16% formaldehyde-EM grade (Polysciences, Inc) and incubated at room temperature for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained using DAPI to label the nucleus and mounted on pre-cleaned glass slides using Aqua-PolyMount reagent (Polysciences). Following indirect immunofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... together with packaging plasmids pCMV-VSV-G and pCMV-delta8.9 using PEI (Polysciences Inc.) 48 hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with Polyethylenimine HCl MAX 4000 (PEI) (Polysciences, Inc.) as described (Reed et al ...
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bioRxiv - Cell Biology 2020Quote: In utero live imaging of oocytes was accomplished by mounting adult worms with a single row or less of embryos in 1.5μl of M9 mixed with 1.5μl of 0.1μm polystyrene Microspheres (Polysciences Inc.) on a 6% agarose pad with a coverslip gently laid over top ...
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bioRxiv - Cell Biology 2020Quote: ... with 1.5μl each of M9 buffer and 0.1μm polystyrene microspheres (Polysciences Inc.) on a microscope slide covered with a coverslip ...
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bioRxiv - Microbiology 2020Quote: ... using the transfection reagent polyethylenimine (PEI, Polysciences). (Note ...
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bioRxiv - Microbiology 2020Quote: ... Embryos were transferred with a glass needle and mounted in ~1.5μL of Boyd buffer containing ~50 polystyrene-based microspheres (20 μm in diameter, Polysciences) between a 24 × 50 mm glass slide and an 18 × 18 mm glass coverslip ...
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bioRxiv - Microbiology 2020Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... before addition of polyethylenimine (PEI MAX (Polysciences, Generon, UK) in a 3:1 ratio (PEI:DNA ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl PEI-max (1 μg/μl; Polysciences) was mixed with DNA (1 μg/ml cells ...
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bioRxiv - Microbiology 2020Quote: ... Plat-E cells were co-transfected with target gene vectors and an amphotropic envelope vector using polyethylenimine (PEI; Polysciences, Warrington, PA, USA) as described previously39 ...
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bioRxiv - Microbiology 2020Quote: ... All samples included 2 μm diameter Fluoresbrite beads (Polysciences Inc., Warrington, PA, USA) for size reference and alignment purposes ...
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bioRxiv - Microbiology 2020Quote: ... at a density of 2.5 × 106 cells/ml were transfected with microbody expression vector plasmid DNA using polyethyleneimine (Polysciences, Inc) at a 1:3 plasmid:PEI ratio ...
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bioRxiv - Microbiology 2020Quote: ... pCAG-HIVgp (5 μg) and pCMV-VSV-G-RSV-Rev (5 μg) (Riken BioResource Research Center) using PEImax solution (60 μL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 μL ...
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bioRxiv - Neuroscience 2020Quote: ... rinsed in PBS for 3 h and embedded in polyethylene glycol (MW = 1500; Polysciences). Six-ųm sagittal sections were cut on a rotary microtome ...
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bioRxiv - Microbiology 2020Quote: ... we used fluorescent polystyrene (PS)-based latex beads (Fluoresbrite® YG Microspheres 1.00 µm, Polysciences, Inc.) as the lowest sized MPs ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 or 293T cells were performed using polyethylenimine (PEI) (Polysciences) transfection ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS-BGT and mounted on coverslips with Aqua Poly/Mount (Polysciences Inc.). Cells were imaged by confocal microscopy using Olympus IX81 laser-scanning confocal microscope using a PlanApo N 60× oil objective with a 1.42 numerical aperture (NA) ...
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bioRxiv - Microbiology 2020Quote: Constructs were introduced into HEK293F cells by high-density transfection with PEI MAX 40K (Polysciences, Inc., Warrington, PA, USA) (47 ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293T cells were performed using the Transporter 5 Transfection Reagent (Polyethylenimine; Polysciences) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Cells were then cross-linked with 1% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA), for 10 min at 26°C followed by another 5 min incubation after adding glycine to a final concentration of 125 mM to stop the cross-linking reaction ...
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bioRxiv - Microbiology 2020Quote: ... then fixed in 4% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA) diluted in PBS for 10 min at room temperature followed by a 5 min incubation with 250 mM glycine to stop the fixation reaction ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Hek293T-cells were transfected with pNL4-3 or pCH058/2960 or pNL4-3 and pMM310 (pBlam-Vpr) via polyethylenimine (Polysciences Inc.)[67] ...
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bioRxiv - Genomics 2020Quote: ... Cells were transfected using the PEI method (Polysciences). Media was changed 12 hours after transfection and viral-containing supernatant was collected 24 and 48 hours later ...
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bioRxiv - Molecular Biology 2020Quote: ... Uvitex-2B (Polysciences Inc., USA), was occasionally used in combination with PI as described in [46] with the following modifications ...
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bioRxiv - Immunology 2020Quote: ... conjugated to 20μm polyethylene carboxylate beads (Polysciences Inc.) with 20μg of polyinosinic:polycytidinic acid (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 30 minutes at RT in a solution of 0.5x Fix Buffer (OXP3 buffer set, eBiosciences, 00-5523-00) supplemented with 1.6% formaldehyde (Polysciences, 18814). Post-fixation ...
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bioRxiv - Immunology 2020Quote: Peeled dermis and epidermis sheets were cut to 1 cm2 and fixed in 2% formaldehyde (Polysciences, 18814) and 30% sucrose in PBS for 24 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2020Quote: ... using polyethylenimine (Polysciences, Warrington, Pennsylvania). Six hours after transfection ...
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bioRxiv - Immunology 2020Quote: A microsphere suspension was prepared by diluting 2 μm fluorescent polystyrene microspheres (Fluoresbite® YG Carboxylate Microspheres, Polysciences, 2.5% microsphere suspension) 1:1 (v/v ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Immunology 2020Quote: ... with PEI MAX (Polysciences) in ProCHO5 medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... with PEI MAX (Polysciences) in EX-CELL 293 serum-free medium (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... with PEI MAX (Polysciences) in ProCHO5 medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... some of the prepared BALF cell spots were fixed and treated with Wright-Giemsa stain (Polysciences, Inc., Warrington, PA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The pTT5-VHH72hFc1X7 plasmid was transiently expressed in CHO55E1 cells (34) using PEI-Max transfection reagent (Polysciences, Warrington, PA) and a slightly modified protocol as described previously (35) ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Immunology 2020Quote: Samples from NHP were depleted using purified DENV or BSA control protein adsorbed onto Polybeads polystyrene microspheres (Polysciences, Inc.) as reported earlier (11 ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... and HeLa cells by Polyethylenimine ‘Max’ (Polysciences, Inc., Germany). Poly (I:C ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Immunology 2020Quote: ... samples were incubated in a 2% formaldehyde solution (Polysciences) for 2 h ...