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Citations for Polysciences :
2901 - 2950 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... fast blue (FB, 1.5 μl 2% in 0.9% saline, Polysciences) or into the left knee with 7.5 μl CFA (10 mg/ml ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Immunology 2020Quote: ... or pαH-SARS-CoV-2 RBD-Fc using 2 mg polyethylenimine (Polysciences, Cat# 24765). 6 days after transfection ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... BioInvent n-CoDeR® scFv were selected using biotinylated in-house produced recombinant LILRB3-hFc recombinant fusion proteins (captured with streptavidin-coated Dynabeads®) with or without competition or LILRB1-hFc coated to etched polystyrene balls (Polysciences, US) or plastic immunotubes ...
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bioRxiv - Immunology 2020Quote: ... HEK293A were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Immunology 2020Quote: ... Fc.IL-2-expressing plasmids (pcDNA3.1) were transiently transfected into HEK 293T/17 adherent cells (ATCC CRL-11268) using PEI 25K (Polysciences, Inc.) (ratio 3:1 ...
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bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml linear polyethylene imine (Polysciences Inc., Warrington, PA, USA) and 0.7 mg of the expression plasmid were incubated in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Biophysics 2020Quote: Fluorescent particles of 1.001 μm diameter (Polysciences) were added to each sample to obtain 0.054% of final particle concentration by volume for all microrheology measurements ...
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bioRxiv - Biochemistry 2020Quote: ... Contrasting for TEM was done by embedding the grids in 1.8% methyl cellulose and 0.4% uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... samples were stained with 1% aqueous uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cell transfections were done using MaxPEI 25K (Polysciences) in a 3:1 MaxPEI:DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and then transfected with 8 μg of an equimolar mixture of the three third-generation packaging plasmids and 8 μg of rTetR-domain library vectors using 50 μl of polyethylenimine (PEI, Polysciences #23966). After 48 hours and 72 hours of incubation ...
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bioRxiv - Genetics 2020Quote: ... slides were mounted with Aqua-Poly/mount (Polysciences Inc, Warrington, USA). Images were obtained with Leica TCS SP5 confocal laser microscope and visualized with Leica Application Suite (LAS ...
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bioRxiv - Genetics 2020Quote: ... 200 µL of 1 µm Fluoresbrite yellow-green (YG) microspheres beads (#17154-10, Polysciences, Inc) diluted 1:4 in sterile DPBS were administered retro-orbitally ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Genetics 2020Quote: ... Third-generation packaging and envelop vectors (pMDL, psRev, and pMD2G) together with Polyethyleneimine (PEI Polysciences PN 23966, in DPBS (Gibco) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... using PEI (Polysciences) of shRNA-expressing viral vectors with the packaging plasmids pCMVR8.74 and pMD2.G ...
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bioRxiv - Cell Biology 2020Quote: Plasmid transfections were performed with polyethylenimine “MAX” (Polysciences, Cat. #24765) when HEK293A cells were grown to 95% confluency ...
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bioRxiv - Immunology 2020Quote: ... pEE12.4-RBD-SpyTag-Histag plasmids were transfected with PEI (PEI MAX-Transfection Grade LinearPolyethylenimine Hydrochloride, Polysciences 24765-1) into 293F cells ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... we confirmed the linearity of data acquisition with beads of known concentration and size (102.7±1.3 nm, Polysciences) comparable with EVs (Supplementary Fig ...
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bioRxiv - Immunology 2020Quote: ... by transient transfection using PEI MAX (Polysciences) or ExpiFectamine™ 293 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... washed in two drops of water and stained 30 s in either 0.75% uranyl formate (UF) or 2% sodium phosphotungstate (NaPT, Polysciences Inc. USA), pH 7.5.
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bioRxiv - Neuroscience 2020Quote: ... and polyethylenimine (PEI, Polysciences) was used for cell transfection at a ratio of 2:1 (PEI ...
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bioRxiv - Neuroscience 2020Quote: ... increasing concentrations of Ardalite 502/PolyBed® (02595-1, Polysciences, Inc) in propylene oxide ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with CRISPR-Cas9 plasmids using PEI-Max (Polysciences Inc.) as described previously (Morciano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... using 0.10 μm polystyrene microspheres (Polybead, Polysciences Inc.) and 10 mM Levamisole (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were mounted with Aqua-Poly/Mount (Polysciences Inc.), and coverslipped (Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then washed in PBS and coverslipped with Aqua-Poly/Mount (Polysciences Inc.) mounting media ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped with Aqua-Poly/Mount (Polysciences, Inc.) mounting media.
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bioRxiv - Immunology 2020Quote: Cell numbers per tissue were determined by mixing 20 µl of each single cell suspension into a 96-well plate with 50 μl of 8.4 × 104 Fluoresbrite Carboxylate YG 10 µm microspheres/ml (Polysciences Inc.) and 180 μl staining media (dPBS + 2% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with a mixture of plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) and Polyethylenimine (PEI) (Polysciences, Inc., 23966) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Cell Biology 2020Quote: ... Polyethylenimine “Max” (24765-1, Polysciences) was used for transfection ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4] containing 0.5% glutaraldehyde (Polysciences, Warrington, PA) for 1–2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were transfected with pAB61Strep or pPlxdc2-FcStrep using Polyethylenimine "Max" (PEI) (Polysciences) (50 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Neuroscience 2020Quote: ... Brain slices were mounted on slides and coverslipped with Aqua Poly/Mount (Polysciences, Cat# 18606). Object slides were stored at 4°C in the dark.
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bioRxiv - Biophysics 2020Quote: ... we dispensed 6 μm diameter polystyrene beads (Polybead microspheres, Polysciences) diluted in HBSS into recording μ-slide channels and let them settle to the bottom of the μ-slide ...
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... C6 were transfected with using PEI reagent (Polysciences, Warrington, PA, USA). UR61 and URMax34 cells were transfected using the Ingenio Electroporation solution (Mirus ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were mounted onto glass slides using AquaPolymount (Polysciences) and dried overnight.
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were fixed with 4% formaldehyde (Polysciences) for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and then mounted in Aqua/Poly Mount (Polysciences). They were imaged with Transmitted Light on a Nikon Diaphot 300 microscope using a 10x objective.