Labshake search
Citations for Polysciences :
2001 - 2050 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Coverslips were mounted with Aqua-Poly/Mount (Polysciences, Inc.), and the slides were stored at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... using a mold (Polysciences, #07918986), frozen and cut into cryosections ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Developmental Biology 2021Quote: ... testes were fixed with Bouin’s fixatives (Polysciences). Slides were stained with PAS and H&E after deparaffinization ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslipped with Aqua-Poly/Mount (Polysciences, Inc.). Slides were then photoprotected and stored at −20◻C until confocal imaging ...
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bioRxiv - Neuroscience 2021Quote: ... 11% (by volume) scattering bead solution (1.00 μm Polybead Microspheres; part no. 07310-15; Polysciences), and 5% (by volume ...
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bioRxiv - Neuroscience 2021Quote: ... Cutaneous afferents innervating both hind paws of male C57BL/6J mice were labelled with the retrograde tracer Fast Blue (2% w/v in sterile PBS; Polysciences) when animals were aged 9 weeks ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then rapidly removed and placed in ice-cold PBS for ~5 minutes before being embedded in Tissue-Tek OCT media (Sukura Finetek Inc, Torrance, CA, USA) in Peel-A-Way cryo-embedding molds (Polysciences, Inc, Warrington, PA, USA) and covered with dry ice ...
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bioRxiv - Neuroscience 2021Quote: ... L4 animals were immobilized with M9 containing 0.05 µm diameter polystyrene beads (Polysciences) and mounted on a 3% agarose pad on a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg/dish each) using PEI MAX (no. 24765-1, Polysciences) into HEK293T cells seeded on eight 15 cm tissue culture dishes ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-RC and pAAV-Helper plasmids into 293T cells using PEI MAX (Polysciences; Pennsylvania, USA). 72 h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... for nuclear labeling and mounted on glass slides with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Neuroscience 2021Quote: ... reconstruction of the recording track and compared to retrogradely labeled PT neurons - FastBlue (Polysciences) injected into the pons - in each animal to limit analysis to units within layer 5B (upper boundary ...
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bioRxiv - Neuroscience 2021Quote: ... and embedded in AquaPolymount (Polysciences, 18606). For chromogenic stainings ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed with 1 μg of DNA per single well of a 6-well plate using PEI MAX (Polysciences, INC.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: Retrovirus packaging cells were transfected with pQCXIP-hMETTL18-HA or pQCXIP-hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA using PEI transfection reagent (Polysciences) and cultured for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... coli suspension in LB medium or a 1% (vol/vol) suspension of 1 μm diameter polystyrene beads (Polysciences 431), diluted in NGM buffer to 0.1% vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... The cells in each well were transfected with 1.6 μg PEI (Polyethylenimine, Linear, MW 25000, Polysciences, U.S.A.) and 400 ng of pMU2-Camk2b plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... were transfected into cells at 80%–90% confluency using polyethyleneimine (Polysciences, U.S.A.). We employed a pAAV ...
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bioRxiv - Neuroscience 2021Quote: ... An aqueous solutions of 25% (wt/vol) Poly(acrylic acid) (Polysciences, MW 50000) was spun at 2000 rpm (WS-650-23 ...
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were mounted using AquaPolymount (18606-20, Polysciences) and stored in the dark at 4°C until microscopic analyses ...
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bioRxiv - Neuroscience 2021Quote: ... or in Bouin’s fixative (Polysciences, Inc.) for anti-hEAAT1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µm polystyrene beads (Polysciences) suspended in 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were mounted with Aqua-poly/mount (Polysciences Inc, 18606-20).
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bioRxiv - Molecular Biology 2021Quote: ... Human embryonic kidney 293A cells were then used to produce full length whole Ab 1116NS19.9 or Ab 5b1 from their respective p3BNC expression plasmids that were transfected with polyethylenimine reagent (PEI; Polysciences). Functionality of cloned secreted 1116NS19.9-hIgG1 and 5b1-hIgG1 antibodies was tested by ELISA against antigen-coated plates.
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bioRxiv - Molecular Biology 2021Quote: ... we used 40 kDa polyethyleneimine (PEI) (Polysciences) with a DNA / polyethyleneimine ratio of 1 μg / 3 μl with a total of 1 mg DNA per 1 L of cells at 1M cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... IKKβ or an empty vector (EV) control using polyethylenimine (Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and mounted in Aqua Poly/Mount (Polysciences Inc) followed by imaging on a Nikon A1R HD.
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bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were performed using PEI (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Immunology 2021Quote: ... or PEI transfection reagent (PolySciences Inc. Cat# 23966) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 48 hours at 37°C after which the media was removed and 150 uls of 10% formaldehyde (Polysciences) was added to inactivate the virus ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5mg of both plasmids were incubated with 3mg of Linear PEI 25K (Polysciences) in 100ml of Opti-MEM (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... Pellets of PET (Polysciences Inc, USA) were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Genetics 2021Quote: ... and were bound to BioMag Plus Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences, Inc). Tissues were then permeabilized for 10min in dbe+ Buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...