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Citations for Polysciences :
851 - 900 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and beta-gal plasmid using PEI Max 40000 (PolySciences, USA). The next day transfection medium was exchanged with fresh medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 67.5 μg of polyethyleneimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 μg of plasmid encoding a spike variant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Substitution with LR–White resin (medium grade; Polysciences, Warrington, PA, USA) was achieved by incubations with 25% to 100% LR–White resin and samples were placed in a polymerization chamber for 72 h at 60°C ...
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bioRxiv - Immunology 2023Quote: ... using polyethylenimine (PEI, Polysciences, Inc.). The cells were fed with 10 mM sodium butyrate and 10 ml 45% D-(+ ...
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bioRxiv - Microbiology 2023Quote: ... with polyethylenimine (PEI) (Polysciences). All computationally designed variants were produced in pCAGGS plasmids encoding the sequence of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were stained with Picrosirius Solution B (Polysciences, 24901B) for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Target-gRNA contained pX458 were transfected into MCF10A cells with PEI MAX (Polysciences Inc). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected with PEI MAX (Polysciences Inc). Primers used for this experiment are listed in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were stained with Weigert’s Hematoxylin Kit (Polysciences, Warrington, USA) for 8 min and washed for 10 min under running tap water ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... Capture of VirB was performed on a 30 mL phosphocellulose (Polysciences, Inc.) column ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were captured with BioMagPlus Concanavalin A (Polysciences) for 10min at room temperature and incubated with primary antibody (final concentration 1:100 ...
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bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
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bioRxiv - Cell Biology 2023Quote: ... Neutravidin-coated beads were prepared by incubating 0.4 ng of 0.8 μm diameter polystyrene beads (Polysciences) and coated with 5 mg/mL neutravidin (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The Transfection mix contained 1µl linear polyethylenimine (PEI, pH 7.3, Polysciences) with a concentration of 1 mg/ml per 100 ng DNA and 0.5 to 1 μg plasmid DNA per well ...
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bioRxiv - Molecular Biology 2023Quote: Equal numbers of HEK293T cells were seeded and the different plasmids were transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... or Aqua Polymount (Polysciences, 186065).
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bioRxiv - Cell Biology 2023Quote: ... Samples were freeze substituted in 0.1– 5% uranyl acetate in acetone and embedded in Lowicryl HM20 (Polysciences) with automated temperature control (Leica Microsystems EM-AFS2) ...
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bioRxiv - Physiology 2023Quote: ... Polyethylenimine (PEI) was obtained from Polysciences (#23966), Type I collagenase was obtained from Worthington Biochemical (LS004196) ...
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bioRxiv - Neuroscience 2023Quote: ... were vortexed with 4–6 µg polyethylenimine (PEI Max, Polysciences, Inc., Warrington, PA) and incubated for 5 min ...
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bioRxiv - Immunology 2023Quote: ... 500 μl of Fluoresbrite YG Carboxylate Microspheres 1.75 μm (PolySciences, 17687-5) were opsonized in 4500 μl of Normal Human Serum (GeminiBio ...
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bioRxiv - Neuroscience 2023Quote: ... Transfection with the plasmid expressing PH-PLCδ::GFP probe was done 24 hours before the imaging experiments using Polyethylenimine solution (PEI; Polysciences Inc.; 1 mg/ml) when the cells were around 70% confluent.
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bioRxiv - Developmental Biology 2023Quote: ... we used 1µM-diameter fluorescent beads (Fluoresbrite® PolyFluor® 570 Microspheres, Polysciences, #24061-10) that were ingested by the worms ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were accomplished using a PEI MAX polyethylenimine reagent (Polysciences; catalog no. 24765). DLD-1 cells originally obtained from ATCC.org (catalog no ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using PEI MAX® polyethylenimine reagent (Polysciences; catalog no. 24765). After transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using PEI MAX® polyethylenimine reagent (Polysciences; catalog no. 24765).
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bioRxiv - Developmental Biology 2023Quote: ... The eggs were then fixed on ice in FHM medium containing 0.25% (v/v) glutaraldehyde (Polysciences, Inc., Warrington, PA) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: ... 293T cells were transfected by Polyethylenimine “Max” (PEI) (Polysciences) transfection with the respective expression plasmids ...
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bioRxiv - Immunology 2023Quote: ... 65 μl of PEI Max 40K (Polysciences) stock solution (1 mg/ml ...
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bioRxiv - Biophysics 2023Quote: Cells were pre-permeabilized in 0.5% Triton X-100 in cytoskeleton buffer for 15 s for tubulin and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 1.6 mL of 40 K polyethylenimine (Polysciences 24765-1) (1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... FreeStyle 293-F cells were transiently transfected using polyethyleneimine (Polysciences, #23966) in Opti-MEM Reduced Serum Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells (ATCC) were transfected with plasmids using PEI ‘MAX’ (Polysciences Inc.) and subjected to electrophysiological assays 16-72 h after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x PBS wash and mount slide/coverslip with Aqua Poly Mount (Polysciences Inc. 18606-20). Imaging was performed on a Zeiss AiryScan2 LSM900 confocal microscope using the ‘airyscan mode’ and following standard operating procedures12.
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bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1 M Phosphate buffer (PB) ...
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bioRxiv - Cell Biology 2023Quote: ... Mounting was performed with Aqua Poly Mount mounting medium (Polysciences, cat. No. 18606).
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bioRxiv - Molecular Biology 2023Quote: ... Sirius Red (cat# 24901250; Polysciences, Inc), or Alcian Blue (cat#A3157 ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiCRISPR v2 plasmids72 were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... pre-saturated with Fast Blue dye solution (FB, Polysciences, Warrington ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl (10^10 microspheres/mL) of Fluoresbrite® Multifluorescent Microspheres 0.2 µm (PolySciences 24050-5) were spread onto a bilayer plate using sterile glass beads and dried for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfections were performed using Polyethylenimine (PEI, Polysciences Inc.). For each transfection ...