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Citations for Polysciences :
851 - 900 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and Sirius Red (cat# 24901250; Polysciences, Inc) staining was performed per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... embedded in Lowicryl HM20 resin (Polysciences, Warrington, PA), UV polymerized at −60°C warming slowly over 4 days to room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescence images were captured as z-projections using a Nikon A1R confocal microscope and analysed by the NIS-Elements software.
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bioRxiv - Bioengineering 2023Quote: ... we added 80 µL of polyethylenimine (Polysciences #23966) to 500 µL of Opti-MEM (Fisher #31985062 ...
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bioRxiv - Biophysics 2023Quote: ... polystyrene reference beads (Polysciences Europe) of 1.5 µm in diameter were diluted 1:1500 in PBS buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and 1.2 ml of PEI (Polysciences) before incubation for 15 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with polyethyleneimine (PEI) (Polysciences; #23966-1) with pCMV-dR8_91 and pVSV-G packaging plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cover slips were then washed again and mounted onto glass slides using Aqua Polymount (Polysciences). Fluorescence microscopy ...
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bioRxiv - Cancer Biology 2023Quote: Transient transfection for fluorescence microscopy was performed directly on coverslips coated with poly-L-lysine (see above) placed in 24-well plates using the transfection reagent Polyethylenimine (PEI; Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 15-micron polystyrene beads (Polysciences Polybead microsphere 15 µm catalogue number 18328-5) ...
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bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transfected into 293T cells using PEI (Polysciences, high potency linear, MW = 40 000) transfection reagent ...
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bioRxiv - Biochemistry 2023Quote: ... in 2 L flasks using polyethylenimine (PEI, Polysciences) reagent according to the manufacturer’s protocol in an incubator at 37°C and 8% CO2 under shaking (125 rpm) ...
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bioRxiv - Microbiology 2023Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... The HEK293T cells were transfected using 1 mg/mL Polyethylenimine (PEI; 23966, Polysciences) in a ratio of 3 μL to 1 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with PHP.eB capsid plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... Producer cells were transfected with a solution containing the selected lentiviral vector transgene backbone, the packaging plasmids pMDLg/pRRE and pCMV.REV, pMD2.G (Plasmid Factory, Bielefeld, Germany) and polyethylenimine (PEI) (24765, Polysciences, Warrington, US). Transfection media was changes after 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Aquamount (Polysciences, 18606) was used to mount coverglass slides ...
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bioRxiv - Neuroscience 2023Quote: ... with 112 μg PEI MAX (Polysciences, Inc.) as a transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: ... 73.3 µl polyethylenimine (0.6 µg/µl, Polysciences 24765- 2). Solutions I and II were combined and incubated at 24 °C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... solution (Polysciences) when necessary and were blocked and permeabilized in 3% BSA with 0.3% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM Spermidine) and bound to 10 µl activated BioMag®Plus Concanavalin A magnetic beads (Polysciences) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... DNA was introduced into cells using polyethyleneimine MAX 40K (PEI MAX) (Polysciences) at a ratio of 3:1 PEI:total DNA or TransIT-LT1 (Mirus ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were counterstained with DAPI and cover slipped with Aqua-Poly/Mount (Polysciences). Tissue sections were imaged on an Axioscan 7 (Zeiss ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips or sections were mounted with Aqua Poly/Mount (Polysciences) and imaged.
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bioRxiv - Neuroscience 2023Quote: ... mounted with Poly Aqua-Mount (Polysciences, 18606), and imaged using a confocal microscope (Zeiss LSM 880 or Zeiss LSM 700).
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted using Polymount (Polysciences,18606-100).
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected using polyethylenimine (PEI) (#23966- 1; Polysciences, Inc.) with 5 μg of the indicated DNA constructs using a 1:5 ratio with PEI ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and coverslipped with Aqua/Poly/Mount (Polysciences). Immunfluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 1µM-diameter fluorescent beads (Fluoresbrite® PolyFluor® 570 Microspheres, Polysciences, #24061-10) that were ingested by the worms ...
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bioRxiv - Developmental Biology 2023Quote: ... The eggs were then fixed on ice in FHM medium containing 0.25% (v/v) glutaraldehyde (Polysciences, Inc., Warrington, PA) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... or Aqua Polymount (Polysciences, 186065).
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bioRxiv - Cell Biology 2023Quote: ... Samples were freeze substituted in 0.1– 5% uranyl acetate in acetone and embedded in Lowicryl HM20 (Polysciences) with automated temperature control (Leica Microsystems EM-AFS2) ...
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bioRxiv - Molecular Biology 2023Quote: Staining of lung sections for fibrillar collagen was performed using the Picrosirius Red Stain Kit following the manufacturer’s instructions (Polysciences, Inc., Warrington PA). Digital morphometric measurements were performed on multiple lobes and multiple levels with ten random peripheral lung images devoid of large airways per slide analyzed at a final magnification of 100× using Image J as published (34 ...
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bioRxiv - Microbiology 2023Quote: ... The Fungi-Fluor® Kit (Polysciences, PA, USA) was applied following the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... using polyethylenimine (PolySciences). Five days post transfection cell culture media were harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... Polyethylenimine (PEI) linear MW 40000 (Polysciences, Inc) was used as the transfection reagent.
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bioRxiv - Molecular Biology 2023Quote: ... Transfection Grade (Polysciences; 23966) and Opti-MEM™ (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection Grade (Polysciences; 23966) and Opti-MEM™ (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and 15 μg PEI (Polysciences Inc., Cat#23966-1, Warrington, PA) in a 1.5 mL Eppendorf tube ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x PBS wash and mount slide/coverslip with Aqua Poly Mount (Polysciences Inc. 18606-20). Imaging was performed on a Zeiss AiryScan2 LSM900 confocal microscope using the ‘airyscan mode’ and following standard operating procedures12.
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bioRxiv - Immunology 2023Quote: ... 3µl of PEI-Max transfection reagent (Polysciences) (1mg/ml ...