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Citations for Polysciences :
751 - 800 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pCAGGS_MERS-CoV-S or pCAGGS_HCoV-229E-S using Polyethylenimin (PEI) Max (MW = 40,000KDa, Polysciences, DE) as transfection reagent at a ratio of 4:1 (PEI:DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The required amount of BioMag Plus Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences, Inc) were transferred into the binding buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Molecular Biology 2023Quote: ... Silica 100 nm microspheres (Polysciences Inc., Philadelphia, United States) were used to quality check the instrument performance daily ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Polyethyleneimine (PEI) was purchased from Polysciences, Inc (Warrington ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Linear poly-ethylenimine (PEI, 25-kDa, # 23966-1) was obtained from Polysciences (Warrington, PA, USA). G418 (#108321-42-2 ...
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bioRxiv - Neuroscience 2023Quote: ... and mounted in Mowiol 4.88 with DAPI (Polysciences). Cells were imaged with a Zeiss CellObserver Z1 widefield fluorescent microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... polyethylenimine (Polysciences) and the lentiviral plasmid of interest ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.25 µg of pcDEF3-hACKR3 and 4.75 µg of empty pcDEF3 DNA were combined with 40 µg of 25 kDa linear polyethylenimine (Polysciences) in a total volume of 500 µL 150 mM NaCl and incubated for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected at a confluence between 50 % and 70 % with 8 μg of plasmid DNA per dish using polyethyleneimine 25,000 MW (Polysciences) solution as described previously (Guaitoli et al. ...
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bioRxiv - Neuroscience 2023Quote: Yellow-green fluorescent spheres (ex 441 nm, em 486 nm, Fluoresbrite® YG Microspheres 0.20 µm, Polysciences, Cat. # 17151) were mounted in 2% agarose in CUBIC-R+ medium (RI 1.52 ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (w/vol) Mowiol 4-88 (Polysciences). Fluorescence images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Cell Biology 2023Quote: ... and pX330 vector expressing Gpx4-targeting sgRNA (sgGpx4) using PEI MAX (Polysciences). Transfected cells were sparsely reseeded and incubated with 2µg/mL puromycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and staining with 1% uranyl acetate (Polysciences, Inc, Warrington, PA).
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid expressing CD5L was transfected into the HEK293F cells by polyethylenimine (Polysciences), and the cells were cultured in SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... pCAG-FIP200NTD (1-640) was co-transfected with pCAG-TSF-ATG13363-517-ULK1MIT (836-1050) using the polyethylenimine (PEI) (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2023Quote: ... cells were initially seeded at 2× 105 per 2 ml in 6-well plates and transfected using PEI MAX 40K (Polysciences Inc. # 24765-100) (1:3 DNA to PEI ratio) ...
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bioRxiv - Cell Biology 2023Quote: ... and psPAX.2 (3.75 μg) with PEI MAX 40K (Polysciences Inc., # 24765-100) (1:3 DNA to PEI ratio) ...
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bioRxiv - Cell Biology 2023Quote: ... and embedded in Mowiol (Polysciences cat. No. 9002-89-5) containing anti-fading agent DABCO (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... PEI (Polysciences) and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293T cells were triple transfected with PEI-MAX (Polysciences, #24765) to deliver the rep-cap or iCAP ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 kDa polyethylenimine (Polysciences), Custom heavy isotope labeled Sulf-2 peptide (Biosynth) ...
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bioRxiv - Biophysics 2023Quote: ... working mixtures of Fluoresbrite YG 0.1µm-diameter Microbeads (Polysciences, Inc, Warrington, PA, USA) and FluoSpheres carboxylate-modified 0.1µm-diameter red (580/605 ...
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bioRxiv - Physiology 2023Quote: ... was purchased from Santa Cruz Biotechnology. Polyethylenimine (PEI; cat. 23966) was purchased from Polysciences. Nonfat dry milk (cat ...
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bioRxiv - Neuroscience 2023Quote: ... Cover slides were mounted using Aqua-Poly/Mount mounting medium (Polysciences) and imaged using confocal microscopy (Leica TCS SP8 confocal).
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bioRxiv - Microbiology 2023Quote: ... using polyethylenimine (Polysciences). All downstream experiments were performed 24 h post-transfection ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Bioengineering 2023Quote: All transfections were performed with polyethyleneimine (PEI) transfection reagent (Polysciences, 23966-1, Warrington, PA, USA) on HEK293T cells at a 1:4 (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... transferred to a pre-fix solution containing 4% formaldehyde (PolySciences) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... JB4 solution A (Polysciences); GoTaq qPCR master mix ...
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bioRxiv - Cell Biology 2023Quote: Plasmid transfection was performed using Polyethylenimine”MAX” (Polysciences, 24765) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... separate samples of 10 kPa solution were made substituted with Nile blue-tagged acrylamide (Polysciences Inc. #25395-100) at 1:1000.
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bioRxiv - Cancer Biology 2023Quote: ... using polyethyleneimine linear (Polysciences, Cat# 23966-1). After 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... and mounted with AquaMount (Polysciences, Inc.). Samples were visualized using a Zeiss AX10 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with 4% PFA and 0.2% glutaraldehyde (Polysciences, Cat # BLI1909-10) in PBS (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2.93 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 300 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with PHP.eB capsid plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with plasmids coding for the capsids ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using Lipofectamine 3000 or Polyethylenimine (PEI) “MAX” reagent (Polysciences, Cat 24765), according to manufacturer’s protocols.
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... or pLV HA-UL38-IRES-Hygro plasmid using linear polyethyleneimine (PEI) (Polysciences, Cat.# 23966). Briefly ...