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Citations for Polysciences :
1101 - 1150 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg plasmid DNA was transfected with polyethylenimine (PEI, Polysciences, 23966) at a ratio of 1:3 (µg DNA ...
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bioRxiv - Microbiology 2023Quote: ... The pallet of each sample was then fixed in Mc Dowell containing paraformaldehyde and glutaraldehyde (Polysciences, Inc., Warrington, PA, USA) and post-fixed with 1% osmium tetroxide (OsO4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... samples were infiltrated and embedded in Lowicryl HM20 (Polysciences) at -4 °C ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... using linear polyethylenimine MW25000 (Polysciences, Cat #23966). We seeded ten million A375 cells per 10 cm dish and transfected with 60 µl polyethylenimine ...
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bioRxiv - Microbiology 2023Quote: ... Samples were infiltrated with increasing PolyBed 812 (Polysciences Inc.) and propylene oxide and placed in embedding capsules (BEEM ...
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bioRxiv - Molecular Biology 2023Quote: ... melanin of part of samples were breached before oil red O staining using Melanin Bleach Kit (Cat # 24883 Polysciences, Inc., Warrington, PA, USA). After washing in distilled water ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated for the binding with Concavalin A magnetic beads (Polysciences) by rotating for 10 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... together with polyethyleneimine (PEI Polysciences PN 23966, in DPBS (Gibco)) ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Bioengineering 2023Quote: ... the filaments were fixed in 2.5% glutaraldehyde (Polysciences, Inc.) diluted in deionized water for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... PEGDMA 1000 (average molecular weight, MW = 1000) was purchased from Polysciences (Washington, PA, USA). Lyophilized fish GelMA was purchased from Gelomics (Brisbane ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transiently transfected using 1.5 µg of Polyethylenimine MAX (PEI) (Polysciences) and 1 µg of plasmid per well in Opti-MEM (Thermo Fisher Scientific) ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... the glass surfaces were treated with 0.5% glutaraldehyde (Polysciences, Inc.) for 30 min ...
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bioRxiv - Biophysics 2023Quote: For optical tweezers microrheology measurements we add a trace amount of 4.5-μm diameter polystyrene microspheres (Polysciences) that are coated with BSA to inhibit non-specific interactions with the DNA and enzymes.
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bioRxiv - Biophysics 2023Quote: ... For this study 1:100 dilution of bead solution in double distilled water (Flurospheres amine-modified, 0.2 μm, yellow-green fluorescent, Microspheres, Polysciences) was used ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... Polyethylenimine (PEI, MW 40,000, cat#: 24765-1) was purchased from Polysciences, Inc ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with 250 μL OptiMEM containing 20 μg of polyethylenimine (PEI – maximum molecular mass of 40,000 Da; Polysciences). This mixture was added to 20.0 × 106 Expi293F GnTI-Cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 2-sulfoethyl methacrylate (PolySciences, Warminster, PA) was added to a final concentration of 5 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
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bioRxiv - Cell Biology 2023Quote: ... using 150 μl of 1mg/ml PEI Max (Polysciences #24765). Expression was boosted with the addition of 10 mM sodium butyrate (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... polystyrene microspheres (diameter 25 μm; Polysciences, Inc) were mounted onto tipless triangular silicon nitride cantilevers (PNP-TR-TL-Au ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of 0.1% (w/v) poly-L-lysine hydrobromide (Polysciences inc.) was applied on the carbon surface of the grid for 90 s ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid expressing CD5L was transfected into the HEK293F cells by polyethylenimine (Polysciences), and the cells were cultured in SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... and staining with 1% uranyl acetate (Polysciences, Inc, Warrington, PA).
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bioRxiv - Cancer Biology 2023Quote: ... Polyethylenimine was from Polysciences, Warrington ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Bioengineering 2023Quote: ... Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB) was purchased from Polysciences. Novec 7500 fluorinated oil was purchased from Iolitec ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids expressing Fcα–J and FcRL4 was co-transfected into the HEK293F cells by polyethylenimine (Polysciences). The cells were cultured at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 293T cells were transfected using linear 25-kDa polyethylenimine (PEI, Polysciences, Inc.), as described previously59.
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were transfected with GPΔmuc and GPΔmucHRV3C plasmid using polyethyleneimine (PEI MAX, Polysciences, Warrington, PA) at a mass ratio of 1:3 PEI MAX:DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were mounted with Aqua-Poly/Mount (Polysciences #18606-20) and slides were imaged on a Nikon A1 confocal microscope with the 40X objective.
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
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bioRxiv - Cell Biology 2023Quote: ... immobilized using Polybeads Microspheres (Polysciences 00876-15), as described previously (69 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 mM Spermidine) and bound to 10 µl activated Concanavalin A magnetic beads (Polysciences) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using polyethyleneimine (PEI, MW 40,000; 1 mg/ml, Polysciences) using 2 µg of plasmid DNA ad 6 µl of PEI ...
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bioRxiv - Cell Biology 2023Quote: ... PEI (linear polyethylenimine) was purchased from Polysciences Inc (23966) ...
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bioRxiv - Cell Biology 2023Quote: ... polyethylenimine (PEI) (Polysciences, 23966) was used at a ratio of 1:3 (DNA:PEI) ...
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bioRxiv - Developmental Biology 2023Quote: ... and mounted with 20 µm polystyrene beads (Polysciences, Inc.) between a slide and coverslip as in (Rodriguez et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and the slides were mounted using Aquapolymount (Cat. #: 18606-20, Polysciences).