Labshake search
Citations for Polysciences :
1051 - 1100 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: In-utero embryos were imaged from whole worms soaked in 0.1 μm polystyrene beads (Polysciences) diluted M9 buffer (22.5mM KH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviruses were produced by transfection of HEK 293T cells with VSV.G and gag-pol plasmids together with the plasmid of interest and PEI (2ug per ug of DNA, Polysciences Inc) in Opti-MEM medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... polyethylenimine hydrochloride (PEI, Polysciences) was used as the transfection reagent ...
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bioRxiv - Genomics 2023Quote: ... then incubated for 10 minutes in 5mL 2X SSC containing 1μL of 5000X fiducial beads solution (Polysciences, #17149-10). The sample was then embedded in a polyacrylamide gel ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Liver fibrosis was assessed by quantifying Picrosirius (Sirius) red-stained area (Polysciences, #24901). For STEM121 immunohistochemistry ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Linear (MW 25,000) was purchased from Polysciences, Inc ...
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bioRxiv - Neuroscience 2023Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4 ...
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bioRxiv - Neuroscience 2023Quote: ... and embedded in Epon (Polysciences, Inc, Warrington, PA, USA). All sections were double stained with 6% uranyl acetate (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... PEI (Polysciences #239661) for transfection was prepared by dissolving 100 mg PEI in 310 mL H2O and titrating the solution to pH 3 ...
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bioRxiv - Pathology 2023Quote: ... a fixed number of nonfluorescent beads (10000, 10-µm polybead carboxylate microspheres from Polysciences) was added to each tube ...
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bioRxiv - Pathology 2023Quote: ... a fixed number of nonfluorescent beads (10,000 10-mmpolybead carboxylate microspheres from Polysciences) was added to each tube ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were mounted on slides and embedded in mowiol (Clariant) or Aqua-Poly/Mount (Polysciences).
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.1% PBT (P0 and P7 tissue) or in 0.3% PBT (adult tissue) and mounted with Aqua Polymount (Polysciences Inc.).
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bioRxiv - Physiology 2023Quote: ... MW250,000 (PolySciences, Inc) in 40-T150mm cell culture plates ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Neuroscience 2023Quote: ... slides were mounted with coverslips using Aqua-Poly/Mount (Polysciences, 18606). The slides were kept in the dark for at least 24 h before microscopic imaging.
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bioRxiv - Physiology 2023Quote: ... pLNC-ER: RAS vector and packaging vectors using 1mg/mL of linear 25 kDa polyethylenimine (PEI, Polysciences). 24 hours following transfection ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted using Aqua-Poly/Mount (Polysciences, Inc., US). Images of the different brain regions were taken on Zeiss LSM880 Airyscan confocal microscope at 40x magnification (zoom factor 0.6 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen in OCT compound (Polysciences), and stored at −80 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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bioRxiv - Biochemistry 2023Quote: ... to cells was 1 µg DNA for 106 cells and the ratio of total DNA to PEI Max transfection agent (Polyethylenimine HCL MAX 40 K linear, Polysciences) for 1 µg DNA for 3 µg transfection agent ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with DABCO (#15154-500, Polysciences, Warrington, PA, USA). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2023Quote: ... bacmid (1 µg/mL culture volume) was mixed with 2 µg PEI 25K (Polysciences, Inc.) per µg bacmid in 200 µL warm PBS and incubated at RT for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: ... Linear polyethyleneimine 25K (PEI; Polysciences Europe GmbH, Germany) was combined with DNA (3 μg PEI per μg of DNA) ...
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bioRxiv - Plant Biology 2023Quote: ... and embedded in epoxy resin (Spurr Low Viscosity Embedding Kit, Polysciences Inc) as described earlier (84) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected using polyethylenimine-MAX (PEI-MAX, PolySciences, 24765-1) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... counterstained for DAPI and cover slipped with Aqua-Poly/Mount mounting media (Polysciences, Inc; Warrington, PA). Slides were scanned on an automated slide scanner (Olympus VS120 ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids using polyethylenimine (PEI) transfection (PEI MAX® MW 40,000, Polysciences) as previously described ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA with the amount of 10 μg was mixed with 30 μg of linear polyethyleneimine (PEI) (23966-100; Polysciences) in 1 ml of serum-free DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... a CRL-focused sgRNA library19 was lentivirally packaged using polyethylenimine (PEI MAX® MW 40,000, Polysciences) transfection of Lenti-X cells and the lentiviral pCMVR8.74 helper (Addgene plasmid # 22036 ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyethylenimine (PEI, Polysciences) was used to transfect plasmids into HEK 293T cells.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4–88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were sliced with a hypodermic needle blade to create cross-sections and mounted in AquaPolymount (Polysciences, Inc.). Images were aquired on a LSM 710 confocal microscope (Carl Zeiss ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... 2.2 μg VSVG) and 36 μl of Polyethylenimine (PEI, Polysciences) transfection reagent was diluted to 500 μl in Opti-MEM™ Reduced serum medium (Gibco™ ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Molecular Biology 2023Quote: ... Silica 100 nm microspheres (Polysciences Inc., Philadelphia, United States) were used to quality check the instrument performance daily ...
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bioRxiv - Molecular Biology 2023Quote: ... Polyethylenimine linear (25 kDa) (cat. 23966-100) was purchased from Polysciences. Mouse anti-human PD-L1 antibody (cat ...
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bioRxiv - Neuroscience 2023Quote: ... Fast Blue (Polysciences, Warrington, PA, USA), 0.1mg diluted in 20μl of normal saline ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS twice for 5 min and mounted with Aqua PolyMount medium (PolySciences). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... were used The slides were again washed and stained with DAPI (Thermo-Fisher, 300 nM) for 10 min prior to mounting with Aqua PolyMount medium (PolySciences).
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bioRxiv - Neuroscience 2023Quote: ... We transfected 1000 ng of DNA per well using PEI-MAX (Polysciences # 24765-100) using a ratio of 1000 ng of DNA ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were mounted using Aqua-Poly/Mount (Polysciences) mounting medium onto microscopy glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed and mounted using Aqua-Poly/Mount (Polysciences) mounting medium onto microscopy glass slides.
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bioRxiv - Neuroscience 2023Quote: ... Skin biopsies were fixed in 4% paraformaldehyde (PFA, Polysciences Europe GmbH) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: HeLa cells were transiently transfected using polyethylenimine (PEI) MW25000 (Polysciences, Warrington, PA, USA) with pCAGGAS vector carrying on the HINT1 variant or the human WT ...
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bioRxiv - Microbiology 2023Quote: ... samples were infiltrated with Lowicryl HM20 (Polysciences Inc) and polymerized using UV light ...
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bioRxiv - Microbiology 2023Quote: ... and post-fixed in 1% osmium tetroxide (Polysciences Inc.) for one hour ...
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bioRxiv - Microbiology 2023Quote: ... PEI max (#24765; Polysciences Inc.) was added at a mass ratio of 1:3 DNA to PEI max ...