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Citations for Polysciences :
701 - 750 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Masson’s Trichome staining was performed per manufacturer instructions using the Masson’s Trichome Stain Kit (Polysciences cat #25088).
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bioRxiv - Developmental Biology 2024Quote: Guts from adult female flies were dissected in PBS and immediately fixed in 4% formaldehyde (FA, EM grade, Polysciences) diluted in PBS for 1h at room temperature (RT ...
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bioRxiv - Developmental Biology 2024Quote: ... tissues were embedded using a Poly/Bed 812 kit (Polysciences) and polymerized in an electron microscope oven (TD-700 ...
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bioRxiv - Developmental Biology 2024Quote: ... the Masson’s Trichrome (MT) assay was performed according to the manufacturer’s instructions (Polysciences, Inc., Warrington, PA, USA) on three testicular sections from each male ...
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Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgutbioRxiv - Developmental Biology 2024Quote: ... The entire gastrointestinal tract was taken and fixed in 1 X PBS plus 8% EM grade formaldehyde (Polysciences) for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... A second droplet of (∼ 10 µl) of a solution of 1 µm polystyrene microspheres (Polysciences, cat. no. 07310-15) was then added and gently mixed ...
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bioRxiv - Developmental Biology 2024Quote: ... a volume of Concanavalin A beads (Polysciences, 86057-3) corresponding to 15 µl per histone mark target was prepared by removing the supernatant on a magnetic rack and washing in 1 ml binding buffer (20 mM HEPES [pH 7.5] ...
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bioRxiv - Developmental Biology 2024Quote: ... BioMag Plus Concanavalin A beads (ConA beads, PolySciences NC1358578) were washed twice with cold bead activation buffer (20mM HEPES pH7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... guts were fixed in situ for 25-30 min at room temperature in 8% formaldehyde (Polysciences 18814-20), 200 mM sodium cacodylate ...
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bioRxiv - Cell Biology 2024Quote: Worms were anesthetized in 0.1 mg/ml tetramisole in 1X M9 buffer on empty NGM plates and mounted on thin 2% agarose pads on glass slides with 0.05 mm Polybead microspheres (Polysciences) for immobilization ...
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bioRxiv - Cell Biology 2024Quote: ... the pFX-Lifeact-EGFP expression vector was transfected into MDCK cells using Polyethylenimine Max (Polysciences, Warrington, PA, USA). To establish cell lines stably expressing CLCa-EGFP ...
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bioRxiv - Cell Biology 2024Quote: ... and 48 μL of Polyethylenimine Max (Polysciences, 24765-2). Media was changed after 16 hr ...
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bioRxiv - Cell Biology 2024Quote: ... in amount ratio 1:1) per 1x106 cells was mixed with a threefold (w/w) of PEI (polyethyleneimine, linear MW 25000, Polysciences) in Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus was produced by co-transfecting Cas9-T2A-BFP-Flag plasmid along with two viral packaging plasmids into HEK293 FT cells using Polyethylenimine transfection reagent (PolySciences, 9002-98-6). Approximately 48 hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 18 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 1,000 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Synthetic Biology 2023Quote: Polyethylenimine (PEI) (Polysciences 23966-2) was used to transfect HEK293FT cells ...
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Rationally designed protein bandpass filters for controlling cellular signaling with chemical inputsbioRxiv - Synthetic Biology 2023Quote: ... and 600 ng of polyethyleneimine (24765-1, Polysciences, Inc.). For drug treatment experiments ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Cell Biology 2023Quote: Transient transfections were carried out with PEI MAX transfection reagent (Polysciences, 24765-1) using 70% confluent U2Os cells.
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... The right bilateral heads of the gastrocnemius and tibialis anterior muscles received 2μL injections of 5.0% fast blue retrograde tracer (Polysciences, 17740) with a 26-gauge 10μL Hamilton microliter syringe (Model #701 ...
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bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed for 20 minutes in PBS and coverslipped with Aquapolymount (Polysciences, Warrington, PA). At least 5 sections per DRG were imaged using a Zeiss imager M2 microscope equipped with an AxioCam 512 mono camera and the ZEN Blue Imaging software (version 2.3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using polyethyleneimine (PEI MAX® MW 40,000, Polysciences, 24765-100) as previously described.56 Viral supernatant was harvested after 72 h and cleared of cellular debris by filtration through a 0.45-µm PES filter ...
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bioRxiv - Neuroscience 2023Quote: ... with PEI Max (Polysciences, Cat # 24765). The medium was changed the next day of transfection and cells were incubated for 3 days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with AAV plasmid and helper plasmid using PEI Max 40000 (PolySciences, USA). Twenty-four hours later ...
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bioRxiv - Cell Biology 2023Quote: ... dehydrated in a graded series of EtOH and embedded in PolyBed® 812 resin (Polysciences). Ultrathin sections (60-80 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were carried out using polyethylenimine (PEI MW 25000, Polysciences). Jurkat E6 (ATCC #TIB-152 ...
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bioRxiv - Cell Biology 2023Quote: ... psPAX and pVSVG using PEI Max (Polysciences). Single clones were isolated by limited dilution and expression of mCherry- TRIM21 was verified by fluorescence microscopy.
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Cell Biology 2023Quote: ... and beta-gal plasmid using PEI Max 40000 (PolySciences, USA). The next day transfection medium was exchanged with fresh medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 67.5 μg of polyethyleneimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 μg of plasmid encoding a spike variant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Substitution with LR–White resin (medium grade; Polysciences, Warrington, PA, USA) was achieved by incubations with 25% to 100% LR–White resin and samples were placed in a polymerization chamber for 72 h at 60°C ...
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bioRxiv - Immunology 2023Quote: ... using polyethylenimine (PEI, Polysciences, Inc.). The cells were fed with 10 mM sodium butyrate and 10 ml 45% D-(+ ...
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bioRxiv - Microbiology 2023Quote: ... with polyethylenimine (PEI) (Polysciences). All computationally designed variants were produced in pCAGGS plasmids encoding the sequence of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were stained with Picrosirius Solution B (Polysciences, 24901B) for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Target-gRNA contained pX458 were transfected into MCF10A cells with PEI MAX (Polysciences Inc). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected with PEI MAX (Polysciences Inc). Primers used for this experiment are listed in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were stained with Weigert’s Hematoxylin Kit (Polysciences, Warrington, USA) for 8 min and washed for 10 min under running tap water ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... Capture of VirB was performed on a 30 mL phosphocellulose (Polysciences, Inc.) column ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... and mounted with AquaMount (Polysciences, Inc.). Samples were visualized using a Zeiss AX10 confocal microscope ...