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Citations for Polysciences :
701 - 750 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... using polyethylenimine reagent (Polysciences) for Myo7A forced-dimer constructs or Lipofectamine (Lipofectamine 2000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... post-fixed in 1% osmiumtetroxide (Polysciences) in 0.1 M cacodylate buffer at room temperature for one hour and washed with 0.1 M cacodylate buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sections were poststained with 4 % uranyl acetate (Polysciences) in 25% ethanol and ‘Reynold’s lead citrate’ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... in 0.1 M cacodylate buffer (Polysciences) pH 7.4 for 30 min and after a change for 3 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: Dissected guts were fixed with 4% formaldehyde (Polysciences), 1% glutaraldehyde (Polysciences ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with one or more constructs using polyethylenimine (PEI, Polysciences, Inc.; Warrington, PA, USA, 23966-2).
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were mounted in Aquapolymount (Polysciences) for imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei corresponding to at least 100 embryos per reaction were incubated with 25 μl of BioMag®Plus Concanavalin A beads (Polysciences) (prepared in Binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... fixed in 4% formaldehyde (16% stock solution, PolySciences 18814-20) in PBS for 25mn at R.T. ...
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bioRxiv - Cell Biology 2024Quote: ... 4.5 μm polystyrene particles (Polysciences, 17135-5) were first coated with streptavidin and next with Concanavalin A-biotin conjugate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... single-round HIV-1 Env-/Vif-/Vpr-/Nef-/CFP reporter virus with 1ug plasmid encoding the G protein from vesicular stomatitis virus (VSV-G) for virion pseudotyping) in 10cm dishes using polyethylenimine (PEI; catalog no. 23966; Polysciences Inc., Warrington, PA, USA). Culture media were replaced at 24 hours post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... using PEI (PolySciences). Virus supernatant was collected after 60h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1% glutaraldehyde (Polysciences) in 0.1 M cacodylate buffer (Polysciences ...
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bioRxiv - Cell Biology 2024Quote: ... or in a mixture of 75:25 RapiClear 1.47 (SUNJin Lab):Aqua-Poly/Mount (Polysciences) for clearing the egg chambers ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Molecular Biology 2023Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were stained with Masson’s trichrome (Polysciences, Inc., Warrington, PA, USA) to assess collagen abundance ...
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bioRxiv - Neuroscience 2023Quote: ... Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) was added and they were covered with a cover glass ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times with DPBS before embedding with Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) and a cover glass ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7.5 μg psPAX2 plasmid using 80 µL of 1mg/mL polyethylenimine (Polysciences, 25000) in 500 μL of Opti-MEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 80uL of PEIMAX (Polysciences #49553-93-7) were then added ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... pMD2-VSV-G and pLKO-shRNA or pWPI expressing NS4B using 25 kDa linear polyethylenimine (Polysciences Inc.) exactly as before (Anton et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were mounted in Aquapolymount mounting medium (Polysciences Inc). For cryosections immunolabeling ...
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bioRxiv - Neuroscience 2023Quote: ... Expression plasmids were transfected using polyethylenimine (PEI) (23966-1, Polysciences). 10 ug and 200 ng of total plasmid was used for 100 mm cell culture dishes and 8-well plates respectively with a Plasmid:PEI ratio of 1:4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: All transfections were performed by incubating DNA with the transfection reagent polyethylenimine (PEI, 25K linear, Polysciences 23966) in a 1:3 ratio in serum free DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... with linear polyethylenimine (PEI) 25 kDa (Polysciences) as transfection reagent and using 4.5 µg DNA and 9 µg PEI per mL of transfection volume ...
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bioRxiv - Molecular Biology 2023Quote: ... and linear polyethylenimine (PEI, 25K, #23966-100, Polysciences, Warrington, PA, USA) were purchased from Life-Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... transfection reagent (Polysciences, no. 23966). After 48 hours ...
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bioRxiv - Physiology 2023Quote: ... Sections were picked up on 1 x 2-mm formvar-coated copper slot grids (Polysciences) and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Systems Biology 2023Quote: ... we added 80 µL of polyethylenimine (Polysciences #23966) to 500 µL of Opti-MEM (Fisher #31985062) ...
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bioRxiv - Systems Biology 2023Quote: ... we added 9.09 uL of polyethylenimine (Polysciences #23966) and incubated for 15 min at room temperature before adding the final mixture for each plasmid to a HEK293-containing well of the 6-well plates ...
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bioRxiv - Microbiology 2023Quote: ... and Mayer’s hematoxylin (Polysciences) was used as a counterstain ...
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bioRxiv - Neuroscience 2023Quote: ... Stained sections were mounted on glass slides with Aqua-Poly/Mount (Polysciences #18606).
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bioRxiv - Neuroscience 2023Quote: 10 cm dish transfection: 9µg of plasmids and 25µL 1µg/µL Polyethylenimine (Polysciences, Warrington, PA) were mixed in 1mL Opti-MEM (Thermo Fisher Scientific ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... 2.2 μg VSVG) and 36 μl of Polyethylenimine (PEI, Polysciences) transfection reagent was diluted to 500 μl in Opti-MEM™ Reduced serum medium (Gibco™ ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Neuroscience 2023Quote: ... and sections were mounted using Aquapolymount mounting solution (Polysciences Inc.). The slides were stored in the dark at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... pUCmini-iCAP-PHP.eB and pHelper plasmid with the plasmid ratio of 1:4:2 based on micrograms of DNA were transfected into cells at >90% confluency with 1mg/ml polyethyleneimine (PEI, Linear, MW 25000, Polysciences). pUCmini-iCAP-PHP.eB for PHP.eB production was a gift from Dr ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4–88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were sliced with a hypodermic needle blade to create cross-sections and mounted in AquaPolymount (Polysciences, Inc.). Images were aquired on a LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected into HEK293FT cells using 2 μg of Polyethylenimine (Polysciences). Lentiviral particle containing media was filtered using 0.45μm cellulose acetate syringe filter and used for transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... linear (PEI; Polysciences) and was used for the T24-GemCis and TCCSUP-GemCis screens ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...