Labshake search
Citations for Polysciences :
901 - 950 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) were incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
-
bioRxiv - Genomics 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery96 ...
-
bioRxiv - Cell Biology 2023Quote: The WT and Akt2 KI mouse eyes were immersed fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, Warrington, PA) and 2% paraformaldehyde (Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Neutravidin-coated beads were prepared by incubating 0.4 ng of 0.8 μm diameter polystyrene beads (Polysciences) and coated with 5 mg/mL neutravidin (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1 M Phosphate buffer (PB) ...
-
bioRxiv - Cell Biology 2023Quote: ... Mounting was performed with Aqua Poly Mount mounting medium (Polysciences, cat. No. 18606).
-
bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sirius Red (cat# 24901250; Polysciences, Inc), or Alcian Blue (cat#A3157 ...
-
bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
-
bioRxiv - Cell Biology 2023Quote: Slices were rinsed and mounted with Aqua Poly/Mount (Polysciences Inc., Washington, USA). Images were acquired by a Leica DM TCS SPE confocal microscope (HC APO 20x/0.75 ...
-
bioRxiv - Biophysics 2023Quote: ... A volume of 20 μL of 25 mM levamisole mixed with 5 μL 200-nm-sized polystyrene beads (Polysciences Inc., Warrington, PA, USA) was dropped onto the cover glass ...
-
bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
-
bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
-
bioRxiv - Systems Biology 2023Quote: ... The stock solution of PEI HCl (PEI MAX 40000; Polysciences Inc; Cat# 24765) was prepared according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... The transfection was performed using linear polyethyleneimine hydrochloride (40,000, PEI MAX, Polysciences, Cat# 49553-93-7). For each vector 5× 15cm dishes were transfected and subsequently AAV particles were purified from cell pellet and media using iodixanol gradient purification as previously reported79.
-
bioRxiv - Physiology 2023Quote: ... The sections were embedded in Spurr resin (Polysciences Inc., Warrington, PA, USA), cut into ultrathin sections ...
-
bioRxiv - Microbiology 2023Quote: The 25kDa PEI 25™ (PEI-25) and 40 kDa PEI Max® (PEI-40) polymers were acquired from Polysciences and were prepared according to manufacturer instructions to get a stock solution of 1 mg/ml ...
-
bioRxiv - Immunology 2023Quote: ... using Polyethylenimine (PEI, Polysciences) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using PEI MAX® polyethylenimine reagent (Polysciences; catalog no. 24765).
-
bioRxiv - Cell Biology 2023Quote: ... Transient transfections were accomplished using a PEI MAX polyethylenimine reagent (Polysciences; catalog no. 24765). DLD-1 cells originally obtained from ATCC.org (catalog no ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using PEI MAX® polyethylenimine reagent (Polysciences; catalog no. 24765). After transfection ...
-
bioRxiv - Immunology 2023Quote: ... 500 μl of Fluoresbrite YG Carboxylate Microspheres 1.75 μm (PolySciences, 17687-5) were opsonized in 4500 μl of Normal Human Serum (GeminiBio ...
-
bioRxiv - Cell Biology 2023Quote: ... The Transfection mix contained 1µl linear polyethylenimine (PEI, pH 7.3, Polysciences) with a concentration of 1 mg/ml per 100 ng DNA and 0.5 to 1 μg plasmid DNA per well ...
-
bioRxiv - Neuroscience 2023Quote: ... were vortexed with 4–6 µg polyethylenimine (PEI Max, Polysciences, Inc., Warrington, PA) and incubated for 5 min ...
-
bioRxiv - Immunology 2023Quote: ... 65 μl of PEI Max 40K (Polysciences) stock solution (1 mg/ml ...
-
bioRxiv - Biophysics 2023Quote: Cells were pre-permeabilized in 0.5% Triton X-100 in cytoskeleton buffer for 15 s for tubulin and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... 1.6 mL of 40 K polyethylenimine (Polysciences 24765-1) (1 mg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... FreeStyle 293-F cells were transiently transfected using polyethyleneimine (Polysciences, #23966) in Opti-MEM Reduced Serum Medium (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... 293T cells were transfected by Polyethylenimine “Max” (PEI) (Polysciences) transfection with the respective expression plasmids ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells (ATCC) were transfected with plasmids using PEI ‘MAX’ (Polysciences Inc.) and subjected to electrophysiological assays 16-72 h after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were captured with BioMagPlus Concanavalin A (Polysciences) for 10min at room temperature and incubated with primary antibody (final concentration 1:100 ...
-
bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Polyethylenimine (PEI) [linearpoly(vinyl alcohol) (MW-25000)] was from Polysciences (Warrington, PA). Molecular biology enzymes and reagents were from Promega (Southampton ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Research Center plasmid RDB04393, provided by Dr. Miyoshi, Keio University) plasmids 45 using a polyethlenimine “Max” (Polysciences Inc.). Virus-containing media were collected 48 h after transfection ...
-
bioRxiv - Developmental Biology 2023Quote: ... Transfections were performed using Polyethylenimine (PEI, Polysciences Inc.). For each transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (29 ...
-
bioRxiv - Neuroscience 2023Quote: ... using polyethylenimine (PEImax, Polysciences Inc, Warrington, PA, USA). Cells were incubated at 37°C for 72 hours then collected ...
-
bioRxiv - Microbiology 2023Quote: ... 300 µl (10^10 microspheres/mL) of Fluoresbrite® Multifluorescent Microspheres 0.2 µm (PolySciences 24050-5) were spread onto a bilayer plate using sterile glass beads and dried for 30 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... and coverslipped with Aqua-Poly/Mount (Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
-
bioRxiv - Immunology 2023Quote: ... and 2.2 μg/ml polyethylenimine (linear 25 kDa PEI, Polysciences, Inc, Warrington, PA) to the suspension cultures ...
-
bioRxiv - Microbiology 2023Quote: ... 500 µL PEI-max (1 µg/µL; Polysciences) was mixed with DNA (1 µg/mL cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... Packaging cells were transfected using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
-
bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
-
bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
-
bioRxiv - Bioengineering 2023Quote: Fluoresbrite® YO carboxylate microspheres 100nm (Polystyrene nanoparticles) were purchased from Polysciences, USA ...
-
bioRxiv - Immunology 2023Quote: Plasmids were transfected in U2OS cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). 15.2 μg of plasmid and 60.8 μg of PEI were diluted in 2ml of Opti-MEM ...
-
bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
-
bioRxiv - Molecular Biology 2023Quote: Equal numbers of HEK293T cells were seeded and the different plasmids were transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...