Labshake search
Citations for Zymo Research :
5651 - 5700 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were added to the fractions and RNA was isolated using the DIrect-zol RNA Miniprep Plus Kit (Zymo cat# R2052). 16uL of RNA isolated from fractions was reverse transcribed (iScript cDNA synthesis kit ...
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bioRxiv - Immunology 2024Quote: Total RNA in frozen lung tissue powders or BMDMs was purified using a Direct-zol RNA MicroPrep Kit (Zymo Reserch) as described by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... We then performed bisulfite conversion on the fragments using the EZ-96 DNA Methylation Lightning MagPrep kit (Zymo Research #D5046) following the manufacturer directions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids expressing GmIFS2-eCFP and GmCHS7 / GmCHIB1 / GmCHR5 / GmHID -eGFP were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 °C ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from cryopreserved or fresh samples using the ZymoBIOMICS MagBead DNA/RNA kit following manufacturer’s instructions (Zymo Research). Fragment sizes in excess of 20Kbp - without significant degradation - were verified by gel electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were isolated from fully grown cultures (1 mL) using a Zymoprep™ Yeast Plasmid Miniprep II kit (Zymo Research) according to the manufacturer’s protocol and the remaining culture was propagated to start the next screening round.
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was then harvested from cells 24 h post-transfection using the Direct-zol RNA Miniprep kits (Zymo Research). To ensure rigor and reproducibility ...
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bioRxiv - Genetics 2024Quote: ... RNA was isolated from 100 mg of the powdered samples using the Zymo RNA miniprep kit (Zymo Research, Freiburg, Germany) following the manufacturer’s manual ...
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bioRxiv - Genetics 2024Quote: ... The fragmented DNA (200 ng) underwent bisulfite conversion using the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA). To construct the DNA library ...
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bioRxiv - Genomics 2024Quote: ... The reaction was stopped by adding sodium dodecyl sulfate to a final concentration of 0.1% and the products were cleaned using the Zymo clean and concentrator kit (Zymo research).
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated via phase separation and purified via Quick-RNA MicroPrep Kit including the optional DNase treatment (Zymo Research). Fifty ng of RNA was converted to cDNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR amplicons (280 bp) were purified using the Clean and Concentrator PCR purification kit (Zymo Research, Irvine, CA, USA) and their quality verified on a 1.5% w/v agarose gel ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from a single section using the Quick-RNA FFPE Miniprep kit with on-column DNase I digestion (Zymo). RNA was analyzed by bioanalyzer and quantified using NanoDrop ...
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bioRxiv - Bioengineering 2024Quote: ... excess genomic lysis buffer (Beta-Mercaptoethanol added) with a volume of 1.6 mL from the Quick-DNA Midiprep Plus kit (Zymo Research) was used instead of BioFluid & Cell Buffer from the Quick-DNA Miniprep Plus kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragmented genomic DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold Kit (Zymo Research, cat. #D5005) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from cell culture pellets using an Agilent Bravo automated liquid handling platform and Quick-DNA/RNA Viral Magbead kit following the manufacturer’s protocol (Zymo Research). Samples were treated with RQ1 RNase-Free DNase following the manufacturer’s protocol (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of genomic DNA from each sample was bisulfite converted using the EZ-96 DNA Methylation Kit (Zymo Research) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products of approximately the correct size were gel extracted and purified using the Zymoclean Gel DNA Recovery Kit (Zymo) for verification by Sanger sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... followed by 20 minutes at 70 °C.Each sample was then purified using the ChIP DNA Clean & Concentrator kit (Zymo, D5205) as per the manufacturer’s instructions for a 100 µl sample ...
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bioRxiv - Genetics 2024Quote: ... RNA was isolated by using TRIzol Reagent and the Direct-zol RNA MiniPrep Kit with DNase treatment according to manufacturer’s provided protocol (Zymo Research). RNA was quantitated using Qubit RNA BR Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... samples were treated with proteinase K and purified using a ChIP DNA Clean and Concentrator kit (Zymo Research, Irvine, CA).
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bioRxiv - Evolutionary Biology 2024Quote: HiBiT peptide mRNA was in vitro transcribed with T7 RNA polymerase and purified using the RNA clean and concentrator kit (Zymo). 30 µL in vitro translation reactions were assembled as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting nuclei pellets were processed for RNA extraction by utilizing the Quick-RNA MicroPrep Kit (R1050, Zymo Research, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: DNA was extracted from fecal samples per the manufacturer’s protocol for the Quick-DNA Fecal/Soil Microbe MiniPrep Kit (Zymo, D6010). Tritrichomonas muris was detected by PCR with previously published primers recognizing Tmu 28S rRNA gene ...
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bioRxiv - Plant Biology 2020Quote: ... and RNA greater than 17 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1017). After checking digestion quality ...
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bioRxiv - Plant Biology 2020Quote: ... RNA less than 200 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1015). Next ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Synthetic Biology 2021Quote: ... Bands of the expected size were cut out and DNA was extracted with a ZymoClean Gel DNA recovery kit (Zymo Research). For all cloning steps ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the RNA volume was brought up to 100 μL with nuclease-free water and was purified away from other reaction components by an RNA Clean & Concentrator kit (Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The completed PCR reactions were pooled and ethanol-precipitated prior to purification using a DNA Clean & Concentrator Kit (DCC-25, Zymo Research). The concentration of the labeled 197-bp PCR products was determined by UV absorbance ...
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bioRxiv - Cell Biology 2020Quote: ... and the RNA was extracted using the Direct-zol™ RNA purification kit following the manufacturer’s protocol for DNase I digestion (Zymo Research). cDNA was synthesized with Maxima Reverse Transcriptase (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2020Quote: ... The parasites stages were homogenized in TRIzol with DNA and total RNA was extracted using the Direct-Zol RNA MiniPrep Plus kit (Zymo Research) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Genomics 2021Quote: ... Fragments between 400-700 bp were excised from the gel and recovered using the ZymoClean Gel DNA Recovery Kit (Zymo Research). The final libraries were quantified using the Qubit dsDNA BR Kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Duplicate PCR reactions of each sample were carried out and the amplified products were pooled using a DNA Clean and Concentrator kit (Zymo Research). The resulting amplified fragments were analyzed via Sanger sequencing using both forward and reverse primers ...
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bioRxiv - Developmental Biology 2021Quote: cDNA preparation and preamplification: RNA isolation of digested samples was performed using the Quick-RNA MicroPrep kit (Zymo Research, Cat #: R1050). In-column DNAse-I treatment was performed for trace DNA removal before eluting RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... the four guide RNA templates were pooled together and concentrated through purification by a DNA Clean and Concentrator kit (Zymo Research).
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2022Quote: ... the reaction was stopped by adding MN Stop Buffer and the nucleosomal DNA isolation was carried out according to the kit protocol (D5220, Zymo Research). Illumina TruSeq PCR free DNA library was prepared without bead-based size selection followed by a preparative size selection on the PippinHT to remove non- ligated adaptors and library molecules with inserts >500 bp.
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2022Quote: ... We followed the PCR with an agarose gel extraction and a column purification using the Oligo Clean & Concentrator kit (Zymo Research). The DNA template was then in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were homogenized in RNA STAT-60 (Tel-Test, Inc., Friendswood, TX) and total RNA purified using a Direct-zol RNA miniprep kit (Zymo Research). We used an RNA Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: ... we used Q5 hot-start high-fidelity DNA polymerase to amplify the transcripts of each candidate gene from complementary DNA (cDNA) synthesized using the total RNA of w1118 fly heads (extracted using MiniPrep kit; Zymo Research). For each candidate gene ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting product was then treated with Turbo DNase and the remaining RNA was purified with the Zymo RCC-25 column purification-25 kit (Zymo Research). The A/B RNA standards were quantified using Qubit RNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and fl/fl from each nest/uterus) that were subsequently cleaned and DNAse treated using the zymo-clean&concentrator kit (Zymo Research). For primary neurons ...