Labshake search
Citations for Zymo Research :
5601 - 5650 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... RNA was extracted from Tibialis anterior (TA) muscles from each group using The Direct-zol RNA MiniPrep kit according to manufacturer’s instructions (Zymo Research). RNA quality for sequencing was determined using the high-sensitivity Agilent RNA ScreenTape Assay for 4200 Tape Station System (Agilent Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA for 16S rDNA amplicon next generational sequencing was isolated using the ZymoBIOMICSTM – 96 DNA Kit (Zymo Research, D4309). The 16S amplicon library was prepared in a 96-well format using dual-index barcodes [80] ...
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bioRxiv - Plant Biology 2024Quote: ... and the DNA contained therein was excised and extracted using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Each transcript extracted was then cloned into a pRACE cloning vector (Clontech Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 300 ng of DNA was treated with bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, D5005, USA) and amplified by PCR using Takara Premix Ex TaqTM Hot Start Version (Takara Research ...
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bioRxiv - Molecular Biology 2024Quote: ... Selected colonies will be transferred to liquid culture and then plasmid isolation will be performed with the ZymoPURE Plasmid Maxiprep Kit (#D4202, ZYMO).
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bioRxiv - Molecular Biology 2024Quote: Pellets were resuspended in 500µL TRIzol reagent and total RNA was extracted using a Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted by Direct-zol RNA Miniprep Plus kit according to the manufacturer’s instruction (Zymo, Catalog no. R2072) and then reverse-transcribed into complementary DNA using the iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from pelleted cells as described previously16 using a ZymoBiomics DNA Miniprep kit (Zymo Research; Irvine, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were then reverse-crosslinked overnight in elution buffer (1 % SDS, 0.1 M NaHCO3) and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated as described and RNA was extracted using the Zymo Quick-RNA MiniPrep Kit (Zymo Research, catalog R1055) following the kit instructions ...
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bioRxiv - Zoology 2024Quote: DNA from the homogenized termite guts was extracted using the Quick-DNA™ Fecal/Soil Microbe Microprep Kit (Zymo Research) following kit instructions ...
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bioRxiv - Zoology 2024Quote: ... The cDNA to be used as a template for PCR amplification was obtained from a mix of ten 1 to 25 days-old CSFB adults by RNA extraction using Quick tissue kit (Zymo) and reverse transcription using the LunaScript® RT SuperMix Kit ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from homogenized mouse skin punch biopsies using a Direct-zol RNA MiniPrep Plus kit (Zymo research, R2072). Bone marrow-derived macrophages were plated at 2×105 cells/mL and treated with PBS or 500 ng/mL PAM3CSK4 (Tocris Bioscience ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from cells sorted into high and low PER1 expression bins was extracted using the FFPE miniprep kit (Zymo). Genomic DNA purified using the FFPE miniprep kit was then cleaned using Axiprep Magnetic Beads (Axygen ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 80-100 mg of tissue with the Direct-Zol RNA extraction kit (Zymo Research, CA, USA) as per the manufacturer guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mg of genomic DNA was treated with sodium bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and lysed by vortexing with 0.5 mm zirconia glass beads before extracting RNA using Zymo Direct-zol kits (Zymo #R2072).
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bioRxiv - Systems Biology 2024Quote: ... and then each sample was applied to a spin column supplied in the RNA clean and concentrator kit (Zymo Research). Samples were centrifuged at 12,500 rcf for 30 seconds ...
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bioRxiv - Genomics 2024Quote: ... 1 ug of genomic DNA was bisulfite (BS)-converted using the EZ DNA Methylation Gold Kit (Zymo Research Cat# D5006) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The product of this reaction was then run on a 0.8% agarose gel and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research) to yield the final Spike DNA fragment ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of sheared DNA was bisulphite-converted using the EZ DNA Methylation-Gold Kit (D5005; Zymo Research, Irvine, CA). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the genomic DNA and purified by DNA Clean & Concentrator Kit (Zymo Research). gRNA plasmids were constructed by T4 PNK phosphorylating (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Products from PCR1 were run on a 2% agarose gel in TAE (Tris-acetate-EDTA) buffer and fragments were extracted using the Zymo Gel DNA recovery Kit (Zymo). Eluted products were pooled in equimolar amounts and were submitted to the UC Davis DNA Technologies and Expression Analysis Core lab for a second round of PCR (PCR2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR bands were cut out from agarose gels and purified with Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA).
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bioRxiv - Synthetic Biology 2024Quote: ... the DNA was used to transform competent EBY-100 yeast with the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast cells were cultured in minimal glucose (SDCAA ...
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bioRxiv - Microbiology 2024Quote: ... DNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The sequencing library was prepared using real-time PCR machines to control cycles and limit PCR chimera formation ...
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bioRxiv - Microbiology 2024Quote: The DNA of the ancestor strains and their clones derived after repeated treatment with phage NP3 or M32 and the respective untreated clones after 72 h growth were isolated using the ZymoBIOMICS DNA Mircoprep Kit (Zymo Research Europe GmbH ...
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bioRxiv - Microbiology 2024Quote: ... after which total RNA was isolated and DNA was removed using the Direct-zol RNA MiniPrep Plus Kit (Zymo Research), and cDNA libraries were generated by SeqCenter using the Stranded Total RNA Prep with Ribo-Zero Plus 563 Microbiome kit (Illumina Inc) ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant was used for the extraction using Mag-Bind® Viral DNA/RNA 96 Kit (Zymo Research, US) using the Kingfisher Robot (KingFisher® Flex ...
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bioRxiv - Neuroscience 2024Quote: The total RNA of SH-SY5Y or differentiated SH-SY5Y cells was extracted following the manufacturer’s instruction using the Quick-DNA/RNA MiniPrep Plus kit (ZYMO RESEARCH). 250 ng of total RNA was used for the reverse transcription of cDNA using TaqMan Reverse Transcription Reagents (applied biosystems) ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from homogenized lung tissues using the Quick-RNA extraction miniprep kit (# R1055 Zymo Research, CA, USA) following the manufacturer’s instructions and the concentration of RNA was measured using the nanodrop machine (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... total RNA was isolated from homogenized lung tissues using the Quick-RNA extraction miniprep kit (# R1055 Zymo Research, CA, USA) following the manufacturer’s instructions and RNA concentration and purity was determined by spectrophotometry using the nanodrop machine (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and the resulting products were purified using the DNA Clean and Concentrator-5 Kit following the manufacturer’s instructions (Zymo Research). In-Fusion reactions were prepared in 5 µL volumes at a 1:3 vector to insert molar ratio ...
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bioRxiv - Microbiology 2024Quote: Frozen stool samples from mice pre- and post-dietary treatment were subjected to DNA extraction using the ZymoBIOMICS DNA Miniprep kit (Zymo), with bead beating used to lyse cells ...
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bioRxiv - Microbiology 2024Quote: ... as well as 16S PCR using Zymo Research Quick-DNA Fecal/Soil Microbe Miniprep Kit (D6010, Zymo Research, Irvine, CA). All animal protocols were approved by the Animal Care Committee of the University of California San Diego and followed the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.
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bioRxiv - Physiology 2024Quote: ... total RNA was extracted from fresh apical-out enteroids using Zymo Direct-Zol RNA miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted from isolated fly heads using the Zymo Direct-Zol RNA miniprep kit (Zymo Research, Irvine, CA). Extracted RNA was quantified on a Nanodrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The RNAs were further purified with phenol/chloroform/isoamyl alcohol mix (pH 6.6) followed by clean-up with RNA Clean & Concentrator Kit (Zymo research). The purified RNAs were reverse transcribed using SuperScript IV (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... and a pool of L3 larvae (A. caninum) using a Quick-DNAÔ HMW MagBead Kit (Zymo Research, Irvine, CA, USA) and modified Solid Tissue extraction protocol (Supplemental Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... were ground to a fine powder under liquid nitrogen and an aliquot was used for RNA extraction using the Direct-zol RNA Miniprep kit (Zymo) with PureLink Plant RNA Reagent (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from a single leaf of 3-week-old T4 Setaria lines using the Directzol RNA kit (Zymo) including on-column DNAse treatment ...
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bioRxiv - Neuroscience 2024Quote: ... Linear PCR products were purified from agarose gel (1%) after electrophoresis with a Zymoclean-Gel DNA Recovery kit (Zymo Research), circularized using DNA Ligation Kit
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from 40 mg leaf tissue using the Direct-zol RNA purification kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sorted cells were directly collected in lysis buffer for total RNA extraction using the Quick-RNA Micro-Prep kit (ZYMO). CD11chigh microglia were collected as cells with Venus fluorescence higher than the median Venus fluorescence of all CD11c+ microglia 18
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from the 12-day old plant seedlings using Direct-zol RNA Miniprep kit (Zymo research, R2050). Library preparation was performed using Nanopore Direct RNA Sequencing Kit (SQK-RNA004 ...
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bioRxiv - Molecular Biology 2024Quote: ... All of these reactions generated a 181 bp amplicon that was cut out from a 2% agarose gel and cleaned using the Zymoclean Gel DNA Recovery Kit (Zymo).
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bioRxiv - Molecular Biology 2024Quote: ... 100 µL of this soma sample was carried forward for RNA isolation with the Zymo QuickRNA MicroPrep Kit (Zymo R1050). RNA was isolated from the 500 µL of neurite lysate in parallel with the same kit ...
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bioRxiv - Microbiology 2024Quote: Total RNA was purified from the cell lysate using TRIzol-LS and a Direct-zol RNA Microprep kit (Zymo Research). RNA-Seq libraries were prepared with TruSeq Stranded Total RNA Library Prep Gold (Illumina) ...