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Citations for Thermo Fisher :
4851 - 4900 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The cells were transfected with purified plasmids using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and cultivated for 48 h after transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... Correct plasmid inserts were validated by digestion with EcoRI and XbaI (FastDigest, Thermo Scientific). The plasmids were verified by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: ... Wild type and mutant plasmids were transformed into MAX Efficiency Stbl2 Competent Cells (Invitrogen) for maxi-DNA preparations ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid concentrations were quantified using ultraviolet (UV) spectrophotometry (NanoDrop ND-1000 instrument, Thermo Fisher) and the molecular weights were used to calculate the number of molecules per μL ...
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bioRxiv - Molecular Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Plasmid expansion was performed in thermo-competent Escherichia coli (Omnimax T1 cells; Thermo Fisher) in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with plasmids utilizing Lipofectamine LTX and LTX Plus Reagent (Thermo Fisher; 500 ng plasmid DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 mRNA was transcribed from pCS2-Cas9 plasmid using mMessage mMachine SP6 Kit (Ambion) and purified by lithium chloride precipitation ...
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bioRxiv - Genomics 2021Quote: ... HEK293T.rKSHV219 cells were reverse transfected with 0.8 μg plasmid DNA using Lipofectamine2000 (Life Technologies) following the manufacturer’s instructions and seeded in 12 or 24-well plates for analysis by live-cell imaging or qRT-PCR as indicated ...
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bioRxiv - Immunology 2021Quote: ... Plasmids containing genes of interest were used to transform E.coli BL21 (DE3) Star (Invitrogen). Recombinant proteins were induced upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 0.8×106 cells were transfected with 1.5µg plasmid DNA using Lipofectamine 2000 (11668019, Invitrogen). Selection with 2µg/ml puromycin (P8833 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid DNA was quantified using a NanoDrop ND-1000 spectrophotometer (ThermoFisher Scientific, Waltham, MA). Plasmids were serially diluted and used as standards for quantitative real-time PCR (29).
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bioRxiv - Microbiology 2022Quote: T293 cells were transfected with plasmid DNA (pGIPZ shPDE12) using Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The vectors were subsequently harvested with the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... The pET30a(+) vectors were harvested using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) at a concentration of 96.7 ng ·μL-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Linearized plasmids were in vitro transcribed using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA polyadenylated using Poly(A ...
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bioRxiv - Neuroscience 2022Quote: ... Linearized plasmids were in vitro transcribed using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA polyadenylated using Poly(A ...
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bioRxiv - Cell Biology 2022Quote: ... or plasmid DNA and siRNA co-transfection experiments were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... then CRISPR/Cas and donor plasmids were transfected using Lipofectamine 2000 (Thermo Fisher Scientific). Two days after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... and the indicated plasmids were co-transfected using 2ug DNA and Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit; Thermo Fisher Scientific), purified (RNeasy MinElute columns ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μg of LRG sgRNA expression plasmid was digested with FastDigest Esp3I (ThermoFisher, FD0454) and gel purified using QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with plasmid using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher). For nucleofection of RNP complexes 4 μl crRNA (100 μM ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.3 μg target plasmid using 36 μL Lipofectamine 3000 (Thermo Fisher Scientific; L3000008). DNA was added to 500 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified from single colonies and verified to contain the desired inserts (Invitrogen). The protocol used for generation of the MaMTH reporter cell lines was described previously (Saraon et al. ...
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bioRxiv - Cell Biology 2022Quote: COS-7 cells were transfected with the plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Transfection conditions are described in each experiment ...
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bioRxiv - Cell Biology 2022Quote: ... and NBPXa-CtermLoxP/HA constructs were first cloned into plasmid backbone (Geneart, Thermofisher Scientific), containing a multi-cloning site with SacII ...
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bioRxiv - Cell Biology 2022Quote: ... The linearized plasmids were treated with 0.2 mg/mL proteinase K (Invitrogen, 25530-015), purified with phenol–chloroform treatment and ethanol precipitation ...
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bioRxiv - Cell Biology 2022Quote: ... 1-2 µg of total plasmid DNA was transfected using lipofectamine 3000 (Thermo Scientific). Cells were trypsinized and replated onto No 1.5 coverglass the following day and analyzed ∼12-16 h later by indirect immunofluorescence ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of plasmid DNA was diluted in 125 μl Opti-MEM (Life Technologies). 4 μl Lipofectamine 2000 were diluted in 125 μl Opti-MEM (Life Technologies ...
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bioRxiv - Genetics 2022Quote: ... 10 µg pX330 plasmids containing gRNA for TP53 and 10 µg ApaLI (Thermo Fisher)-linerized TP53-ReCOIN targeting donors were electroporated into about 1 million PFFs ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were transfected to HEK cells in OPTI-MEM using lipofectamine 2000 (Invitrogen) at 70-80% confluency.
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bioRxiv - Neuroscience 2022Quote: ... neurons were transfected with plasmid using Lipofectamine 2000 (Invitrogen, catalog no. 11-668-019) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Successful plasmids were quantified with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen) and diluted from 108 copies to 100 copies ...
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bioRxiv - Microbiology 2022Quote: ... The restriction enzymes and ligase used in plasmid construction were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and then inserted into plasmid pSMT3-LxEGFP restricted with BamHI and HindIII (Thermo Scientific) using T4 DNA ligase ...
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bioRxiv - Neuroscience 2022Quote: ... AAV.DUX4 mutant constructs.V5 or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were harvested 24 hours post-transfection in RIPA buffer containing 10 mM Tris-Cl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of luciferase reporter plasmid pPASPORT constructs was carried out using Lipofectamine 2000 (Thermofisher) according to the user manual ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected in equimolar ratios in high glucose DMEM (Gibco™, Dublin, Ireland) supplemented with 2% FBS and 1% antibiotic/antimycotic solution ...
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bioRxiv - Plant Biology 2022Quote: ... the amiRMYB68 was introduced in the pKGWFS7 destiny plasmid using the Gateway® (Invitrogen) recombination system,
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bioRxiv - Cell Biology 2022Quote: ... All plasmids used for injection were purified using a DNA Miniprep Kit (PureLink, Invitrogen), or a DNA midiprep kit (Macherey Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... WT mESCs were transfected with these plasmids and pgk-puro using Lipofectamine 2000 (Thermofisher), transfected cells were enriched using transient puromycin (Sigma Adrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... 600 ng of each sgRNA plasmid were mixed together with 0.5 μL BpiI (ThermoFisher), 1 μL of 10X Buffer G (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Their PCR products were cloned into pJET1.2 blunt plasmids (Thermo Fisher Scientific, Massachusetts, USA) and sequenced.
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bioRxiv - Plant Biology 2024Quote: ... genes were inserted into the ChampionTM pET300-NT-DEST plasmid (ThermoFisher Scientific, Waltham, MA), before being transformed into Rosetta(DE3 ...
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bioRxiv - Immunology 2023Quote: ... and the fused cDNA was cloned into the pcDNA3.1 expression plasmid (Invitrogen, Calsbad, CA). For visiualization of EROS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transiently transfected with the indicated plasmids using Lipofectamine 2000 or 3000(Invitrogen) as per manufacturer’s instruction.
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bioRxiv - Immunology 2024Quote: ... equal mass of heavy and light chain plasmids were transfected into Expi293F cells (Gibco) per manufacturer’s instructions and harvested 6 days after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... Linearized plasmids were subjected to invitro transcription using a SP6 Transcription Kit (Invitrogen AM1340). The resulting mRNA was purified (Total RNA Kit I ...