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Citations for Thermo Fisher :
4901 - 4950 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Plasmid maintenance and amplification was carried out using Escherichia coli TOP10© (ThermoFisher Scientific). All PrP constructs were generated by standard PCR cloning techniques and are based on the coding region of mouse PRNP (GenBank accession number M18070 ...
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bioRxiv - Microbiology 2023Quote: ... plasmids were mixed in 500 µl of Opti-MEM I reduced serum medium (Gibco) and incubated with 18 µl of TransIT-LT1 Reagent (Mirus ...
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bioRxiv - Cell Biology 2024Quote: All cells were transfected with plasmids using the Lipofectamine™ 3000 Transfection Reagent (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Control vector or PITAR OE plasmids were transfected using Lipofectamine 2000 (Life Technologies, U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... they were transfected with the reporter plasmid in the presence of jetPEI reagent (Invitrogen). They were incubated for 14 h and then rinsed with fresh medium ...
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bioRxiv - Developmental Biology 2024Quote: ... pME-EYFP and p3E-polyA plasmids using Gateway LR Clonase II Enzyme Mix (Invitrogen). We generated miR-144/451::EYFP transgenic line using Tol2 transposon system (REF) ...
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bioRxiv - Cell Biology 2024Quote: ... Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Genetics 2024Quote: ... HEK-293 cells were transfected with respective DNA plasmids and Lipofectamine 2000 (Life Technologies) for 48 hours before lysis ...
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bioRxiv - Bioengineering 2023Quote: ... which was achieved by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Cat.#. K0502). Plasmid isolation protocol was applied according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... gene fragments were amplified from plasmids by PCR using Platinum Pfx DNA polymerase (ThermoFisher). Constructs were assembled by Gibson assembly using the Gibson Assembly® Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transfected into HEK293-T or LNCaP cells using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids for Cas9 and homology repair were transfected to cells with Lipofectamine 2000 (Invitrogen). Successful C-terminal knock-in cells were identified by fluorescence activated cell sorting (FACS ...
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bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... Each resultant plasmid was co-transfected with ViraPower™ Lentiviral Packaging Mix (ThermoFisher # K496000) into 293FT cells to make virus particles ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid backbone pACYCDuet-1 was digested with HindIII/PstI (Fast digest, Thermo Fisher). The DNA fragment encoding the arabinose promoter was amplified with the primers araC-HindIII-F/ParaBAD-mcr-1-R ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were transfected with plasmids using Lipofectamine 3000 (Thermo Fisher Scientific #L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... HyPer7.24 The cDNA of pCS2+MLS-HyPer7 was purchased from Addgene (Plasmid ID: 136470) and was transfected in HAECs using the NeonTM Transfection System (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2022Quote: ... 750ng plasmid and 750ng pack mix were mixed in 200uL serum-free OptiMEM (Gibco) along with 2-8uL PEI ...
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bioRxiv - Microbiology 2023Quote: The ACE2-6xHis or spike expression plasmid was transfected into Expi293F cells (A14635, ThermoFisher) using polyethyleneimine MAX (PEI MAX ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid was transfected into 293T cells with Lipofectamine™ 2000 (Thermo Fisher Scientific) and the cells were harvested 24 hr post transfection for subsequent analysis.
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with the linearized plasmid DNA-lipofectamine LTX complex (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The restriction enzymes and ligases used in plasmid construction were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected for 8 h with indicated plasmids in Lipofectamine 3000 (Invitrogen) and then allowed for 24-h recovery from transfection by changing a fresh medium before subsequent experiments.
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bioRxiv - Neuroscience 2023Quote: ... and transfected with plasmids as indicated in the text using Lipofectamine 2000 (Thermo Fisher). Pre-warmed 1,6-Hexanediol was diluted to final concentration of 3% in DMEM (culture media ...
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bioRxiv - Synthetic Biology 2023Quote: ... and each well was transfected with 4 μg plasmid DNA using Lipofectamine 3000 (Invitrogen) as a transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... 600 ng VSV-G envelope plasmid and 6 μL of Lipofectamine 2000 (Thermo Scientific). Supernatants were collected at 48 and 72 h post-transfection and clarified using 0.45 μm filters ...
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bioRxiv - Systems Biology 2023Quote: ... We transfected cells using 1 ug of reporter plasmid and TurboFect™ (Thermo Fisher) transfection reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... and (3) OE-GNA13 or NC plasmids using Lipofectamine™ 2000 (Thermo Fisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of isolated plasmid DNA was spectrophotometrically determined using a NanoDrop 1000 (ThermoFisher, Scientific) and DNA aliquots were stored at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... These plasmids were transfected on both primary and C2C7 myoblasts with TurboFect (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting plasmid was introduced into RPE1 by transfection with Lipofectamine 3000 (ThermoFisher Scientific). Following a 4 d selection in 2 µg/ml puromycin ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid concentrations were measured spectrophotometrically at 260 nm via a Nanodrop 2000 (Thermo Scientific) and diluted to <1.0 OD units if necessary ...
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bioRxiv - Cell Biology 2023Quote: Plasmid maintenance and amplification was carried out using Escherichia coli TOP10© (ThermoFisher Scientific). All PrP constructs were generated by standard PCR cloning techniques and are based on the coding region of mouse PRNP (GenBank accession number M18070 ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was created by the transduction of each plasmid with Lipofectamine 3000 (Invitrogen, L3000075) into HEK293T cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with plasmid DNAs using polyethylenimine (PEI, Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... we transfected the template plasmid using Lipofectamine™ LTX reagents (Thermo Fisher Scientific, 15338100). The medium was changed and synthetic sgRNA transfected using Lipofectamine™ RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: RNA constructs of interest were amplified from a parent plasmid (gene blocks from ThermoFisher) and inserted into a linearized pcDNA3.0 using Gibson assembly ...
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bioRxiv - Bioengineering 2023Quote: The plasmid was transfected into HEK293 cells using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 5 ug of plasmid DNA were placed in 250 μL OptiMEM media (Gibco, 31985062), then 7.5 μL of transit-LTI reagent was added to DNA/OptiMEM solution ...
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bioRxiv - Cancer Biology 2022Quote: Transfections of plasmid DNA in HEK293T cells were performed using Lipofectamine® 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was cleaned from plasmid contamination using TURBO DNA-free™ Kit (Invitrogen, AM1907) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection of plasmids or siRNA duplexes was performed with Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 7.5 μg GEM2 donor plasmid using the Neon Transfection System (Thermo Fisher Scientific) with 3 × 10 ms pulses at 1300 V ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 2 μg plasmid using Lipofectamine 3000 (Thermo Fisher Scientific) for HeLa cells or Polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... PacI (Thermo) digested adenoviral plasmids were transfected into 293A cells using Lipofectamine 2000 (Invitrogen). 293A cells were cultured in DMEM (Sigma 5796) ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA (150 ng/ well) was added to the Opti-MEM (Invitrogen; 31985088), followed by the addition of 1.25 μg/well of PEI lipid and incubated at room temperature for 30 min before being added to cells.
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bioRxiv - Genomics 2022Quote: To transfect the plasmids overexpressing the transcription factors we used Lipofectamine 3000 (Invitrogen L3000001) reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...