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Citations for Thermo Fisher :
4551 - 4593 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
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bioRxiv - Cancer Biology 2024Quote: ... or a spheroid TSM-complete for 3D culture (Peterziel et al., 2022) into 6 well plates (Greiner, 657160 for adherent culture and Thermo Fisher, 174932 for suspension culture). Collagen coating was employed for 2D culture to facilitate attachment for the first passage ...
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bioRxiv - Immunology 2024Quote: ... The amplification reaction was performed using ChamQ SYBR qPCR Master Mix (Vazyme) in the QuantStudio 6 Flex System (Thermo Fisher Scientific, Waltham, MA, United States). The primers for the experiment were bought from Sangon Biotech Co. ...
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bioRxiv - Genomics 2022Quote: ... and 0.5 µl primer mix were added in triplicate to a 384-well plate and the reactions were carried out by the QuanStudio 6 Flex (Applied Biosystems, Foster City, CA, USA). The conditions were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... the transwell was moved to a standard 6-well cell culture plate and submerged in 10% neutral-buffered formalin (Fisher Scientific Cat. #22-026-435) for at least 24h ...
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bioRxiv - Microbiology 2023Quote: Quantitative polymerase chain reaction (qPCR) was performed on extracted DNA using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Luna® Universal qPCR Master Mix and primer sets BT2156-BT2160 were used to quantify copy numbers of glucoraphanin-metabolizing genes previously identified from Bacteroides thetaiotaomicron ...
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bioRxiv - Developmental Biology 2023Quote: Primary human limbal epithelial stem cells were isolated from healthy donors and cultivated in 6 well plates in KSFM medium (Cat. Nr. 17005042, Thermo Fisher, Gibco, Life Technologies, Paisley, UK) supplemented with 500 ng Epidermal growth factor (EGF) ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... the ORF4-6 genomic region was amplified directly from the clinical samples using the SuperScript™ IV One-Step RT-PCR System (Thermo Fisher Scientific, Waltham, MA). Primers were designed using Primer3 (26 ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR reactions were carried out in technical triplicates using SYBR Green with a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Genomics 2023Quote: ... Guide RNA and Cas9 ribonucleoprotein complexes were transfected into Hepa1-6 cells using Lipofectamine™ CRISPRMAX™ Cas9 Transfection reagent (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBST and stained for 1 hour with primary antibodies (ZEB1: Bethyl, IHC-00419; E-Cadherin: BD Biosciences, 610181; Collagen 6: Abcam, ab182744; αSMA: Thermo Fisher Scientific, 14-9760-82) and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Bioengineering 2024Quote: All qPCR reactions were performed in an Applied Biosystems QuantStudio 6 Flex using Applied Biosystems MicroAmp EnduraPlate Optical 384-well plates (Thermo Fisher Scientific, Waltham, MA, USA). Reactions were performed at 10 µL ...
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bioRxiv - Cell Biology 2024Quote: ... #329-88-6) and the protein concentration of each lysate was measured with Bicinchoninic acid assay (BCA assay, Thermo Scientific, MA, USA, #23328 and #23224) as previously described [18] ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 6 µL of each sample and separated by high pH reversed phase peptide separation kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten fractions were collected by centrifugation ...
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bioRxiv - Genomics 2020Quote: ... Extracted fungal masses were plunged into a solid medium (7.8 g/L potato dextrose agar [BD], 6 g/L agar [Difco], 100 mg/L Penicillin/Streptomycin [Gibco], and 100 mg/L Kanamycin [Alfa Asear]) and maintained at 28°C for 15 days to screen for possible contaminants and indications of sample viability ...
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were secured with a glass anchor in the bottom of a 35 mm petri dish and incubated in carbogen (5% CO2 and 95% oxygen) bubbled 3,3’-Diaminobenzidine (DAB; ACROS Organics; code: 328005000, CAS: 7411-49-6) solution (1 mg ml-1 in PBS ...
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bioRxiv - Immunology 2019Quote: ... eBioscience Mouse IL-6 ELISA Ready-SET-Go! Kit (Fisher Scientific #50-112-8863; IL-1 beta Mouse Uncoated ELISA Kit (Thermo Fisher Scientific #88-7013-88).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides (100 ng of acyl-BSA) were loaded onto an Acclaim PepMap100 C18 trap column (Thermo Scientific, Product #16-494-6, 75μm x 2cm, 100 Å) and separated on an Acclaim PepMap RSLC C18 analytical column (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... Relative RNA expression was examined by qRT-PCR using a SYBR Green Master Mix (VAZYME, China) on the ABI QuantStudio 6 system (Applied Biosystems, Thermo Fisher Scientific, Foster City, USA). Subsequently ...
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bioRxiv - Genetics 2020Quote: ... Quantitative PCR reactions were performed using the gene-specific primers of FLC and FT (Table S1) with Hieff™ qPCR SYBR® Green Master Mix (Low Rox Plus, China) on a QuantStudio 6 Flex PCR (Life Technologies Corporation, CA, USA). The qPCR signals were normalized to that of the reference gene PP2A in Arabidopsis by using the ΔCT method ...
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bioRxiv - Evolutionary Biology 2022Quote: ... egg sacs were removed from F1 gravid females using a fine needle and placed individually in wells of Falcon® 6-well plates (Thermo Fisher Scientific, Waltham, MA, USA). This was done for 10 SDxSC and 10 SCxSD egg sacs ...
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bioRxiv - Genetics 2022Quote: ... cells were maintained at 37°C and 6% CO2 in 25 mM (high) glucose DMEM (Thermo Fisher # 11995-065 with 1 mM sodium pyruvate; or Thermo Fisher # 11965-092 without pyruvate) supplemented with 10% v/v FBS (Thermo Fisher # 10437-036 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then cultured with the indicated compound for a total of 6-days before cell lysis and quantification of proliferation using the Quant-iT™ PicoGreen dsDNA Assay Kit (ThermoFisher Scientific, Grand Island, NY, USA). For long-term proliferation (12-days) ...
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bioRxiv - Developmental Biology 2023Quote: Primary human limbal epithelial stem cells were isolated from healthy donors and cultivated in 6 well plates in KSFM medium (Cat. Nr. 17005042, Thermo Fisher, Gibco, Life Technologies, Paisley, UK) supplemented with 500 ng Epidermal growth factor (EGF) ...
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bioRxiv - Cell Biology 2023Quote: ... polysome buffer (10 mM Tris, 10 mM MgCl2, 100 mM KCl, 6 mM β-mercaptoethanol, 100 μg/ml cycloheximide, protease inhibitors (Thermo Scientific™, Cat. no. A32961) pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
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bioRxiv - Genetics 2020Quote: 10 μl protein extract was mixed with 6 μl dilution buffer (1x PBS with 20% glycerol) and 2 μl streptavidin solution (Invitrogen, dissolved at 10 mg/ml in PBS), and incubated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature in a holding chamber containing oxygenated imaging media (50% MEM, 50% HBSS, 6 mg/mL D-glucose, and 25 mM HEPES; all from Invitrogen/ThermoFisher; adapted from 77 to be serum-free).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Physiology 2022Quote: ... and RT-qPCR performed using PowerUp™ SYBR™ Green Master Mix on the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems™, Foster City, CA). LinReg PCR analysis software was used to determine the efficiency of amplification from raw CT data (37) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen, Carlsbad, CA, USA, dilution 1:400), for 2 hours at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... were co-nucleofected into 1×106 primed hiPSCs using the Lonza 4D-nucleofector (“Primary Cell P3” solution, “CM-113” program) and plated on a feeder layer of irradiated CF1 MEFs (Thermofisher, 4×106 MEFs per 6 well plate) at 8×g104 nucleofected cells per well ...
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bioRxiv - Pathology 2023Quote: ... which was previously labeled at its 5’ end with one of four specific fluorescent dyes (6-FAM®, NED®, PETTM and VICTM; Thermo Fisher Scientific, Waltham, MA, U.S.A) (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) analysis was performed using the Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific Inc., Waltham, MA, USA). Analysis of endosymbiont DNA was performed using the SYBR Green PCR Master Mix (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... and gene expression quantified by a standard SYBR-based approach (PowerUp™ SYBR™ Green Master Mix for qPCR, Applied Biosystems Cat#A25741, QuanStudio™ 6 Felx Real-Time PCR System, 384-well, Applied Biosystems Cat#4485691).
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bioRxiv - Immunology 2020Quote: ... 293T cells were transfected with either polyethyleneimine (PEI, 3.75 μ per well of 6-well plate with 1.5 µg DNA) or Lipofectamine 2000 (Invitrogen, 1.25 µg DNA per well of 12-well plate) according to manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... qRT–PCR was performed using qPCRBIO SyGreen Mix (Protech) and the QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems/Thermo Fisher Scientific Inc., Waltham, MA, USA). Relative gene expression was determined by the ΔΔCq method ...