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Citations for Thermo Fisher :
4251 - 4300 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected for 16 h with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio prior to actinomycin D chase.
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bioRxiv - Molecular Biology 2024Quote: ... or 2.5 µg (6-well plates) of plasmid DNA diluted in OptiMEM medium using the Lipofectamine 3000 reagent (Invitrogen, ThermoFisher Scientific, France) for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: Sigma Aldrich’s CHOZN cells were cultured in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) supplemented with 6 mM GlutaMAX (Gibco, Waltham, MA, USA), shaking at 37°C ...
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bioRxiv - Microbiology 2020Quote: Lysates of infected HeLa cells were prepared by scraping three wells of a 6-well plate into 300-500 μL volume of ice-cold Mammalian Protein Extraction Reagent (M-PER™) buffer (Thermo Fisher Scientific) supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Molecular Biology 2021Quote: ... A similar protocol was followed for small scale virus production when establishing individual stable cell lines with transfection being performed on 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) reagent.
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bioRxiv - Immunology 2022Quote: ... containing 100 µl of tempus RNA- stabilizing solution aliquoted from a regular-sized tempus tube (designed for the collection of 3 ml of blood and containing 6 ml of solution; ThermoFisher, Waltham, MA, USA). This method is described in detail in an earlier report (7) ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexing quantification used the TMT-128 and TMT-131 channels from a 6-plexing tandem mass tag (TMT) kit (Thermo Fisher, Waltham, MA). See details in the SI document.
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bioRxiv - Neuroscience 2021Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples with RIN≥6 were processed for microarray as described (19) and hybridized onto Affymetrix HT HG-U133PM+ arrays (Affymetrix, Santa Clara, CA) using a GeneTitanR according to Affymetrix technical protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: BMDMs obtained by standard procedure were seeded into 6-well plates (1X106 cells per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM glutamine ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Genomics 2020Quote: Motility agar plates were prepared by adding 20 mL of BHI with 0.3% agar per well of a Corning Costar 6-well plate (Thermo Fisher Scientific, Waltham, MA). C ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 6 Flex Real-Time PCR System using SYBR Green Power SYBR™ Green PCR-Master-Mix (Applied Biosystems™) and primers listed in Supplementary Table 2.
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bioRxiv - Evolutionary Biology 2019Quote: ... and 6 μl of DEPC-treated water using the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, US). Amplification products were visualized in a 2% agarose gel and photographed under the same parameters for each gene.
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bioRxiv - Cancer Biology 2019Quote: Transfections were performed of cells seeded in 6-well plates using DharmaFECT1 as per manufacturer’s protocol (Dharmacon; Thermo Fisher Scientific Inc., Waltham, MA). DUSP4 (M-003963-03 ...
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bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were systematically sectioned (6 μm thickness) using a rotary microtome (Microm HM355S, MICROM International GmbH, Germany) and were mounted on Polysine®slides (Thermo Fisher Scientific). Slides were stored at 4 °C for a maximum of one week before use.
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bioRxiv - Cell Biology 2021Quote: Following a 90 min feed with 5% chicken egg yolk, guts were dissected from larvae (6 dpf, 10 guts pooled per sample) and stored in RNAlater (Thermo Fisher Scientific AM7020) at 4°C for 1 week ...
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bioRxiv - Biochemistry 2021Quote: ... A673 cells (5.0 × 105) in 6-well dishes were reverse transfected with siRNA (50 nM) using Lipofectamine™ RNAiMAX (Invitrogen, cat. no.13778075) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). Cells were washed once with water and suspended into 75 mL ice cold water ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Bioengineering 2020Quote: ... the ADSC and the HUVEC were added after trypsin detachment (both always used at passages 1-6) and centrifugation 1 minute at 3,500 rpm (Minispin, ThermoFisher Scientific, Whaltam, MA, USA) to get a final cell concentration of 4×106 ADSC/mL and 2×106 HUVEC/mL ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was used for real-time quantitative PCR using the SYBR Green Master mix in a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific, 4309155) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was performed in a QuantStudio™ 6 Flex Real-Time PCR system instrument (384-well, Applied Biosystems, Foster City, CA) with an initial incubation at 95°C for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... then replenished with either 200 μL (chamber slide cells) or 6 mL (150 mm dish cells) of Opti-MEM I (ThermoFisher Scientific, Cat#31985062). BioPORTER medium mix was added at a 1:1 volume ratio in the cells grown in chamber slides and a 1:3 volume ratio in the cells grown in 150 mm dishes ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then counted and 500 cells per treatment were plated in triplicate in 6 well plates (Nunc, Thermo Fisher, UK, Cat: 10119831) and incubated for 12 days ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then counted and 500 cells per treatment were plated in triplicate in 6 well plates (Nunc, Thermo Fisher, UK, Cat: 10119831) and incubated for 12 days ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed once with PBS and before mounting on objective glass with 6% n-propyl gallate diluted in glycerol and 10x PBS and combined with ShandonTM Immuno-MountTM (Thermo Scientific, cat# 9990402) in a 1:12 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µl of siRNA or 6 µl RNAiMAX were each mixed with 125 µl of Gibco Roswell Park Memorial Institute (RPMI) 1640 Medium (11875093, Thermo Fisher Scientific Inc.) and incubated separately at room temperature for 2 min ...
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bioRxiv - Cell Biology 2021Quote: ... EndoC-βH1 cells were plated at a density of 1.37□105 cells/cm2 in 24 or 6-well plates and were transfected after 24h by using Lipofectamine 2000 (cat. 11668-019, Invitrogen, Waltham, MA, USA) for 48h with 25 nM of CTR siRNA (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... 12 μg of total protein of each cell lysate was labelled with 6 μg of NHS activated DyLight 630 dye (Thermo Scientific, Waltham, USA) in 50 μl of labelling buffer (50 mM boric acid/ 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...