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Citations for Thermo Fisher :
4451 - 4500 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were seeded into 6 well plates and transfected with 1ug plasmid mixture of pX459-gRNA1 and pX459-gRNA2 using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. 11668019). On the next day ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific, A38524500) in a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific).Both batches were sequenced in a NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression was measured using real time qPCR with PowerUp SYBR Green Master mix on a QuantStudio 6 instrument (Thermo Fisher Scientific, Waltham, MA). Results were normalized to RPS9 (ribosomal protein S9 ...
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bioRxiv - Microbiology 2019Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Bioengineering 2020Quote: A single colony of the desired strain was inoculated in 25 mL pH 6 buffered Glycerol-complex (BMGY) medium (Pichia Expression Kit, Life Technologies, Carlsbad, CA, USA) and grown at 28 °C with 250 RPM shaking until saturation ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Pathology 2021Quote: ... the cells also were washed with PBS and transferred to either aIL-6 antibody (10 ug/ml) or IgG (10 ug/ml) in serum-free DMEM (Thermo Fisher Scientific, Gibco #11966025) supplemented with 4.5 g/L D-glucose ...
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bioRxiv - Bioengineering 2020Quote: ... of the paraffin-embedded spinal cord tissue blocks were cut at a thickness of 6-7 μm using semi-automatic motorized microtome (HM 355, Thermo Fisher Scientific, Kalamazoo, MI). The sections were stained Cresyl Violet (Nissl stain ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: The expression of mRNA transcripts was determined using cDNA reverse-transcribed from total RNA and quantitative PCR (qPCR) was performed on the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) using SYBR Green master mixes (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 104 MDCK-5KO-EKARrEV-NLS-loxP-NRG1 or MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2 cells were seeded in a 6-well plate (No. 140675, Thermo Fisher Scientific, Waltham, MA) and cultured in DMEM containing 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells were seeded in 6-well plates (5×105/well) were transfected with the indicated siRNA (3 μg) using Lipofectamine® 2000 transfection reagent (Life Technologies, 11668-019) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents, Fisher Scientific) in a Fujifilm LAS-3000 imager ...
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bioRxiv - Synthetic Biology 2021Quote: ... 384-well square- or round-bottom microplates were used (Sup Table 6) and covered by an adhesive plate seal (Thermo Scientific #AB0558 or #232701) to minimize evaporation ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Immunology 2021Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Microbiology 2021Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:1,000; Thermo Fisher, HYB-076-08-02), rabbit anti-CFP-10 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed hearts were embedded in paraffin and sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific #630-0950). Immunohistochemistry was carried out using standard procedures ...
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bioRxiv - Cancer Biology 2022Quote: ... HN-6 cells were plated overnight at 40% confluence and transfected with siNC or siSMYD3 using Lipofectamine RNAimax (Thermo Fisher Scientific, Grand Island, NY) for 72h ...
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bioRxiv - Biochemistry 2022Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 g for 30 minutes, A23-6 x 100 rotor, (Thermo Fisher Scientific, Waltham, MA). The supernatant was filtrated with a Filtropur BT50 0.2 µm bottle top filter (SARSTEDT ...
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bioRxiv - Bioengineering 2022Quote: ... Rat bone marrow mononuclear cells were cultured at 4-6 million in a 10cm dish (Falcon) using differentiation media containing: DMEM (ThermoFisher Scientific, Burlington, ON, Canada), 20% HI FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). Cells were washed once with water and suspended in 75 mL of ice-cold water ...
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bioRxiv - Plant Biology 2022Quote: ... respectively) were amplified by PCR from sunflower CAS-6 genomic DNA using the Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and they were cloned into the pMBL-T vector (CANVAX Biotech ...
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bioRxiv - Plant Biology 2022Quote: Replicate trials of soil grown grafted plants from 3-7 days post-grafting were cut at their root tips and dipped into (5 mg/mL) of 5(-and-6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen®, Waltham, MA, USA). Plants were incubated under full-spectrum LED lights (Cirrus LED Grow Lights ...
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bioRxiv - Neuroscience 2023Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin-labeled axons were histologically detected using Alexa Fluor 594-conjugated Streptavidin (Thermo Fisher, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Physiology 2022Quote: ... maintenance medium was added to differentiated adipocytes on day 6 post-induction of differentiation (300,000 preadipocytes were plated per well of a 6-well plate before the induction of differentiation) was supplemented with 50 μM of ManAZ (Thermo Fisher Scientific, Cat #C33366) for 48h ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Developmental Biology 2022Quote: ... was placed into a 6-well tissue culture dish (0.8-1.0ml) and the 6-well plate fitted with Falcon™ Cell Culture Inserts with 8 um pore size (Fisher Scientific, 08-771-20). The lungs were transferred from the holding medium onto the membrane with a sterile razor blade and 5-10 ul of holding media to keep the lungs wet ...
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bioRxiv - Physiology 2022Quote: ... 125 mL of medium was centrifuged at 8000 rpm (9730 x g) for 15 min (Sorvall RC 6 Plus; Thermo Scientific, Waltham, MA, USA). The supernatant was discarded ...
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bioRxiv - Microbiology 2022Quote: THY medium was made using the premixed powders of Todd Hewitt Broth (BD Bacto Dehydrated Culture Media: Todd Hewitt Broth, Fisher Scientific, DF0492-17-6) and yeast extract (Thermo Scientific™ Yeast Extract ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Immunology 2024Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Evolutionary Biology 2024Quote: ... and 5 μL of SsoADV SYBR® Green Supermix (Bio Rad) in a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems™) and following ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of cDNAs were amplified in 1X TaqMan™ Fast Advanced Master Mix and 1X TaqMan™ Assay in a 20-mL reaction using the QuantStudio 6 Flex real-time PCR Platform (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 6 min and 10 min parallel reaction monitoring (PRM) methods are described (UltiMate 3000 RSLCnano and PRM–QExactive Plus; Thermo Scientific, Waltham, MA, USA). Raw data were processed using TraceFinder 4.1 (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... KM-met released from CS-NP was quantified at 233 nm using a NanoDrop One microvolume UV-Vis spectrophotometer for up to 6 hours at room temperature (Thermofisher Scientific, Waltham, MA, USA). At the endpoint ...
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bioRxiv - Developmental Biology 2023Quote: Primary human limbal epithelial stem cells were isolated from healthy donors and cultivated in 6 well plates in KSFM medium (Cat. Nr. 17005042, Thermo Fisher, Gibco, Life Technologies, Paisley, UK) supplemented with 500 ng Epidermal growth factor (EGF) ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate was collected in duplicate from TCPS 6-well plates or Alvetex 12-well inserts following ten days of differentiation at 37°C by RIPA Buffer (Thermo Fisher: 50-152-132) containing 1% proteinase inhibitor (Sigma ...
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bioRxiv - Physiology 2023Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Genomics 2023Quote: ... Relative mRNA levels were determined using qPCR reactions performed on the QuantStudio™ 6 Flex Real-Time PCR System with TaqMan™ Fast Advanced Master Mix (Thermo Fisher Scientific) and taqman probes designed to Cdkn1a (Mm04205640_g1 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were thawed and seeded at a cell density of 5*10^6 cells/ml in RPMI 1640 GlutaMAX™ culture medium (Gibco, Cat.No. 61870-010) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... were plated in 6 well plates and 24 h later each siRNA (17 nM) was transfected with Lipofectamine™ RNAiMAX (Thermo Fisher Scientific, 13778150) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR reactions were carried out in technical triplicates using SYBR Green with a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...