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Citations for Thermo Fisher :
4201 - 4250 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed on a Thermo Fisher Scientific QuantStudio 6 machine using Fast SYBR™ Green Master Mix (Thermo Fisher Scientific 4385618). Expression data is presented after calculating the relative expression compared with the housekeeping gene RPLP0 ...
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bioRxiv - Cell Biology 2023Quote: ... were treated with 10 µM EdU for 6 hours before dissociation and staining using the Click-It EdU Alexa Fluor 488 Flow Cytometry Assay kit (ThermoFisher, Cat# C10425).
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bioRxiv - Plant Biology 2023Quote: ... Relative enrichment levels were determined by ChIP-qPCR in an optical 384-well plate in the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific), using FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded at a density of 1.75×105 cells per well in a 6-well plate were transfected with 40 nM of pooled anti-IL6ST (cat# s7317, s7318, s7319; Thermo Fisher Scientific) or non-targeting Silencer Select siRNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei was then isolated using a sucrose gradient and ultracentrifugation at 13,000 rcf for 45 mins at 4°C (Sorvall WX-100, F65L-6 x 13.5 rotor, Thermo Fisher, Waltham, MA USA). Isolated nuclei were resuspended in Nuclei PURE Prep kit storage buffer and RNAase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Immunology 2023Quote: ... Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific) by using 18S rRNA for normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Molecular Biology 2023Quote: The undifferentiated (Undiff.) and differentiated (Diff.) SH-SY5Y cells seeded in 6 well plates were homogenized in T-PER reagent (ThermoFisher, Cat# 78510), 1% Triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: ... 6 μL was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 μm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Biochemistry 2023Quote: ... –6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific). The data was collected on a Titan Krios at the Pacific Northwest Cryo-EM Center (PNCC ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 μl of RNA from each fraction was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814). The cDNA was diluted 10 times before qPCR analysis ...
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bioRxiv - Cell Biology 2023Quote: ... 2 x 105 cells were seeded in a 6-well plate containing coverslips and transfected with 1 µg dsRNA mixed with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were seeded in 6-well plates and immediately transfected with a mixture of 10 μL Lipofectamine2000 (Thermo Fisher Scientific), 6 μL of 20 μM target-specific siRNA (or non-targeting siRNA as control) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... Single-axis tilt series were collected covering an angular range from -65° to +65° or -60° to +60° at 6- to 8-μm underfocus using the Tomography software package (Thermo Fisher Scientific). Subtomogram averaging was done the eman 2.99 software package ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: LN-229 cells were seeded at 2×10^5 cells/well into in 6-well Nunc™ Cell-Culture Treated Multidishes (ThermoFisher, #140675) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Immunology 2024Quote: MonoMac-6 effector cells were stained with 1 μM of CellTrace™ Far Red dye (CTFR, cat. no. C34564, Thermo Fisher Scientific) in PBS (cat ...
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bioRxiv - Developmental Biology 2024Quote: ... ECs isolated from each mouse were seeded in 2 wells of 6-well plate coated with attachment factor protein 1X (ThermoFisher Scientific, S006100). For a pure EC population ...
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bioRxiv - Cell Biology 2024Quote: ... In tissues β-galactosidase activity at pH 6 was determined using either Senescence β-Galactosidase Staining Kit (cell signaling, 9860) or the CellEvent Senescence Green Detection Kit (Invitrogen, C10850) according to the conditions.
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bioRxiv - Genetics 2024Quote: ... RT-PCR products were fractionated by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (ThermoFisher Scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ v1.47 (NIH ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected for 16 h with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio prior to actinomycin D chase.
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bioRxiv - Biophysics 2024Quote: ... Neuro2A-Piezo1ko cells were transiently transfected 40-48 hours prior to recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were co-stained with Draq5 (Cell Signal, 4048L) or nuclei stain 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI; Thermo Fisher, D1306). Stained samples were imaged using an Olympus FV3000 Confocal Laser Scanning Microscope.
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was first processed by centrifugation at 500 and 2000 × g for 5 min each and 10,000 × g for 20 min using a SorvallTM LYNX 6000 centrifuge equipped with a FiberliteTM F12-6 × 500 LEX fixed-angle rotor (both Thermo Fisher Scientific). All centrifugation steps were performed at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... hESC were diluted at ratios between 1:6 and 1:50 using 0.5 mM EDTA-PBS (Thermo Fisher Scientific, catalog no. 15575020).
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bioRxiv - Physiology 2024Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22, murine IL-6: Invitrogen 88-7064-88) and a SpectraMax M2 plate reader ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to amplify specific circRNAs using its divergent primer pairs in QuantStudio 3/6 Real-Time PCR System (Thermo Fisher Scientific). The delta-CT method was used to check for the relative abundance of individual circular RNA molecules ...
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bioRxiv - Plant Biology 2024Quote: ... were used to amplify a 1:16 dilution of cDNA in triplicate in a Quantstudio™6 PRO real-time thermal cycler (ThermoFisher, Inc.) with a 61°C annealing temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and the same number of cells were re-plated in 6-well plates and selected with G418 (800 µg/ml) (11811031, Thermo Fisher scientific) for 8-14 days ...
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bioRxiv - Neuroscience 2024Quote: ... iNeurons were resuspended and plated at 600K/well in PLO-lam coated 6-well plates in Neurobasal medium (NBM; Fisher Scientific, 11570556), supplemented with 200 mM GlutaMAX (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... the cell debris was sedimented using ultracentrifugation at 100000xg (SorvallTM WX+, Thermo Scientific with A-27 fixed angle rotor 6 x 50mL). The supernatant was then filtered using 0.2 nm syringe cap filters ...
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bioRxiv - Molecular Biology 2024Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/min for 6 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical column ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO) on ABI QuantStudio 6 flex Real-time PCR System (Thermo Fisher Scientific). The RT-qPCR Primer sequences for targeting respective genes are displayed in Table S1 ...
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bioRxiv - Immunology 2024Quote: ... Cleaned skin was then cut in small pieces with scissors and digested in 6 ml of CO2 independent medium (Gibco 18045-054) containing Hyaluronidase type IS 500 µg/ml (Sigma-Aldrich H3506) ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using a PowerUp SYBR Green Master Mix and standard protocols on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific). RPL19 was used to normalize RNA isolated from mouse samples ...
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bioRxiv - Molecular Biology 2024Quote: ... An additional size-fractionation step was performed for all EV and NVEP fractions using a Novex TBE PAGE gel (6%, Invitrogen, Cat# EC6265BOX) with a size range of 150 – 400 bp for cell line-derived cells and EVs ...