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Citations for Thermo Fisher :
4501 - 4550 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 1×106 HL-60 cells were seeded on cell 75 cm2 culture flask in 6 mL IMDM medium (Thermo Fisher Scientific, Waltham, MA, US) and incubated for 24 h in the presence of compound 1 at 10 nM and 10 μM concentrations ...
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bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
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bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Allele sizes were identified by fragment analysis using fluorescent primers (6-FAM, PET, NED, and VIC) and a 3730xl Genetic Analyzer with the LIZ-500 size standard (Applied Biosystems, operated by Genewiz). Alleles were called using Peak Scanner 2.0 (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: A live/dead assay on the cell-laden GelMa constructs (n=6 per condition) was performed using a LIVE/DEADTM Cell Imaging kit (Invitrogen, Waltham, Massachusetts, United States) 144 h after Gel synthesis ...
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bioRxiv - Cell Biology 2023Quote: siRNA probes targeting sfrp2 in human mesenchymal cells and frizzled-5 and -6 in AEC2 cells were obtained from Thermo Fisher (Supplemental Table 4). In brief ...
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bioRxiv - Immunology 2023Quote: ... Cells were counted using a Cellometer Auto T4 Automated Cell Counter (Nexcelom Bioscience LLC, MA, USA) and plated on 6 well-plates (Nunclon Delta Surface, Thermo Fisher Scientific; Cat #140675) precoated with 500 μg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight incubation in a cold room (6-7°C) was carried and anti-mouse/rabbit secondary antibody Alexa-488 conjugated (Thermo Fisher Scientific, Waltham, MA) incubation was performed (1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of cDNAs were amplified in 1X TaqMan™ Fast Advanced Master Mix and 1X TaqMan™ Assay in a 20-µL reaction using the QuantStudio 6 Flex real-time PCR Platform (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After several wash and centrifugations, nuclei were suspended in a solution (phosphate=buffered saline containing 1% 4’, 6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Eugene, OR, USA), and nuclei density was determined in a hemocytometer ...
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bioRxiv - Physiology 2024Quote: ... control siRNA or siRNA targeting ADAR1 (30 pmol per well in 6-well plate) and Lipofectamine RNAiMAX transfection reagent (9 µL; Cat. # 13778075; Thermo Fisher Scientific, Dreieich, Germany) were used to transfect the cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... A total volume of 10µl per reaction was run on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems Thermo Fisher Scientific, 4485691) for 45 cycles (2 minutes at 50 °C ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 1:10 for quantitative real-time PCR (qPCR) run on an Applied Biosystems QuantStudio™ 6 fast real-time PCR system (Applied Biosystems, Foster City, CA) using PowerUp SYBR green master mix (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using TransStart Top Green qPCR SuperMix (TransGen Biotech) on a QuantStudio TM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with the following thermal cycling parameters ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1: 25 and 6 µl was used for one qPCR reaction with PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific, A25776). Relative gene expression was calculated using the ΔΔCT method relative to ACTB control.
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time qPCR reactions were carried on using power up SYBR green master mix on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Microbiology 2021Quote: ... was added to the Lysing Matrix E tube and supplemented with 6 µL of lysozyme (50 mg mL-1, Thermo Fisher Scientific, Cat. no. 90082), followed by a 60 min incubation at 37°C with mixing at 300 rpm ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific, Scornsby, VIC, AUS), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cell Biology 2022Quote: ... collected every 8 hours from 24 to 72 hours post-stimulation on day 6 were lysed in radioimmunoprecipitation (RIPA) lysis buffer (Pierce, Thermo Fisher Scientific; Cat. No.: 89900) containing protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Several donor colonies and one recipient colony were independently inoculated in 2 mL of LB in 15-mL tubes and cultured for 6 hours (37°C and 250 rpm, Thermo Scientific™ MaxQ™ 8000). Cultures were centrifuged (15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM) and underwent blasticidin (ThermoFisher Scientific; 10 μg/ml) selection for 6 days ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from issues of C57BL/6 mice and isolated distal convoluted tubule (DCT) cells of parvalbumin-GFP mice using Trizol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... qPCR assays were performed using the Kapa SYBR Fast Universal 2X qPCR Master Mix (Geneworks, Thebarton, South Australia, Australia) on the QuantStudio 6 Flex (Applied Biosystems, Foster City, CA, USA) following these steps ...
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bioRxiv - Genetics 2019Quote: ... One μL of diluted cDNA (1:25) was used for real-time PCR in a QuantStuioTM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, California, USA). Peptidylprolyl isomerase A (PPIA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The polymer solution was added dropwise to 16 mL citric acid solution 250 mM at pH 2.0 at 300 mOsmol/kg under sonication in an ice bath using the following parameters: initial amplitude 50 (6 mm sonotrode, Fisher Scientific Model 705 Sonic Dismembrator) during the addition of the oil phase (approx ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 6 samples were prepared for proteomics analyses by means EasyPepTM Mini MS Sample Prep Kit (Thermo Fisher Scientific, Rock ford, IL, USA) for proteins extraction ...