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Citations for Thermo Fisher :
4401 - 4450 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies, Darmstadt, Germany) according to the company’s guidelines.
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Molecular Biology 2023Quote: Real-time PCR was performed as reported previously (Tan et al., 2019) using the QuantStudio 6 Flex Real-Time PCR System (ABI, Thermo Fisher, Shanghai, China) and Roche LightCycler® 480 (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... A total volume of 10µl per reaction was run on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems Thermo Fisher Scientific, 4485691) for 45 cycles (2 minutes at 50 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and mouse (n=6; kept on HFD for 28 weeks) livers were measured with the GeneChipTM miRNA 4.0 Array (Applied Biosystems, Foster City, US).
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from hind limb muscle of adult extensor digitorum longus (3-6 months) or newborns (P0) with TRIzol reagent (Thermo Fisher Scientific, 15596026) (41 ...
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bioRxiv - Genetics 2024Quote: ... and TaqMan Assay probes were loaded in technical quadruplicates for qRT-PCR on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485691). The ΔΔCt method was used to provide gene expression values after normalizing to the known reference gene Gapdh ...
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bioRxiv - Cell Biology 2024Quote: ... Cell aggregates were transferred into ultra-low attachment 6-well plates in Complete KSR EB medium (KnockOut™ SR (Life Technologies, #10828-028) 20% ...
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bioRxiv - Cell Biology 2024Quote: ... tissues from 6 different zebrafish were pooled in each condition and we used TriZol protocol to extract total RNA (Invitrogen, Carlsbad, California, USA). The protocol conditions for sample preparation and quantification have been previously detailed (Seiliez and al ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with the secondary antibody sections were washed three times for 6 min in PBS and mounted on microscopic slides covered with mounting dye with DAPI (Invitrogen, 00-4959-52).
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells stably expressing dCas9-GFP-3xFKBP were seeded in 6 well plates (120 000 cells/well, Thermo Fisher Scientific, Waltham, MA, USA) the day before lentivirus transduction ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µg of proteins were separated in each well using 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (ThermoFisher St. Louis, MO) and transferred to PVDF (polyvinylidene ...
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bioRxiv - Bioengineering 2024Quote: ... were complexed for 20 minutes with 6 μL of TransIT-X2 in 50 μL of Opti-MEM™ Reduced Serum Medium (Gibco, Cat# 31985062). After complexing ...
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bioRxiv - Cell Biology 2024Quote: ... 15 000 cells/cm2 were plated in a 6-wells plate and after one day transfected using Lipofectamine 2000 (Thermofisher, Cat no. 11668030) in OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: For nuclei and F-actin staining were visualized with 4’,6-diamidino-2-phenylindole (DAPI) (#D523, Dojindo; 1:1000 dilution) and phalloidin Alexa Fluor plus 405-conjugated (#A30104, Invitrogen; 1:400 dilution), phalloidin ATTO 565-conjugated (#94072 ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). GAPDH (AT1G13440 ...
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bioRxiv - Microbiology 2020Quote: Cells in 6- and 12-well format were washed with PBS and treated with 10 mM TrypLE™ Express Enzyme (Gibco; Cat. No. 12605028). Cells were then collected with PBS and added to 10% PFA to a final concentration of 4% ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 105 rabbit Sf1 epithelial cells were plated into each well of a 6-well plate and incubated at 37°C overnight in a 5% CO2 atmosphere within a HeraCell 150 incubator (Thermo Fisher Scientific, Waltham, MA). At the same time ...
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bioRxiv - Immunology 2021Quote: Human T cells were differentiated for 6–7 days in a humidified 5% CO2 incubator at 37°C with IMDM (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The cells are then transferred with warm hNSC medium to a 6-well plate coated with Geltrex (Gibco, A1413202, incubate for 1hr at 37C). The day after transfection the medium was refreshed and 1µg/ml puromycin (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Neuroscience 2019Quote: ... ∼25000 astrocytes were plated per 30 mm glass coverslip in 6-well plates and allowed to form micro-dot islands in DMEM (Thermo Fisher Scientific, Cat #11965092) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of interleukin (IL)-6 was performed using TaqMan Gene Expression Assays (Rn01410330_m1; Applied Biosystems, Foster City, CA, USA) and 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2019Quote: ... Bacteria were harvested by centrifugation for 10 minutes at 4800 rpm (Thermo Lynx 4000 F12-6 rotor, Thermo Fisher Scientific, Waltham, MA, United States). The obtained pellets were stored at −80 °C until needed ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... the 5’ end of the forward primer sequences for RheBAT26 and RheD18S58 MSI markers was labeled with a 6-FAM fluorescent dye (Thermo Fisher Scientific, Waltham, MA). A multiplex PCR was designed to amplify RheBAT26 and RheD18S58 MSI markers with labeled primer sequences ...
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bioRxiv - Plant Biology 2020Quote: ... 20 ng of reverse transcribed RNA was used as template in the real-time PCR using Applied Biosystems(tm) QuantStudio(tm) 6 Flex (Fisher Scientific GmbH, Schwerte, Germany). Specific primers binding the 3’-UTR of the NOS terminator were used for the detection of the transgene transcripts ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time qPCR reactions were carried on using power up SYBR green master mix on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 37°C in normoxia (21% O2) for Balb/c MSC or hypoxia (5% O2) for C57BL/6 MSC in culture medium consisting of MEM-α (ThermoFisher Scientific, Dublin, Ireland) supplemented with 10% heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... wound lysate cell suspensions were sorted from Tie-2Cre/CXCL12loxP/loxP and CXCL12loxP/loxP transgenic mice as single progenitor cells into each well of a 96-well plate using a Becton Dickinson FACSAria flow cytometer (Franklin Lakes, NJ) into 6 μl of lysis buffer and SUPERase-In RNAse inhibitor (Applied Biosystems, Foster City, CA) (N=5 mice) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using the QuantStudio 6 Flex Real-Time PCR System and Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA). Probe sets for Il6 (Rn01410330_m1) ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was blotted for 25 s with a force of 6 and then flash-frozen in liquid ethane and stored in liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, USA) with the environmental chamber set at 100% humidity ...
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bioRxiv - Biophysics 2022Quote: Human adipose-derived stem cells (hASCs, passage p<=6) were obtained from the Radboud Biobank and cultured in Minimum Essential Medium Eagle (α-MEM) (Invitrogen, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 1.6 μL of the RNA extracts were used for RNA measurement with the use of the NanoDrop™ 2000 Spectrophotometer (Thermo Fisher Scientific, UK). The RNA samples were then stored at -80 °C.
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bioRxiv - Microbiology 2020Quote: ... Then RNA was reverse transcribed into cDNA and the transcriptional levels of different genes were determined by qRT-PCR using SybrGreen Premix Low rox (MDbio, China) and the QuantStudio™ 6 Flex (Thermo Fisher Scientific, America). 16S rRNA was used as an internal reference and the merF gene expression was calculated using the 2−ΔΔCt method ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...