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Citations for Thermo Fisher :
4301 - 4350 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Peptides were dissolved in 25 μl 0.1% trifluoroacetic acid and 6 μl of the extracted peptides was analyzed by Orbitrap Q Exactive HF-X (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS 6 times for 5 minutes each followed by incubation with goat anti-rabbit IgG Alexa Fluor 594 (#A32740, ThermoFisher Scientific, Waltham, MA) and goat anti-mouse IgG Alexa Fluor 488 (#A11008 ...
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bioRxiv - Microbiology 2021Quote: ... Each siRNA including negative control siRNA at an amount of 20 pmol was transfected into cells with 6 µL Lipofectamine RNAiMAX Reagent (Life technologies, 13778-150). 24 h later ...
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bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
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bioRxiv - Cancer Biology 2021Quote: ... For dewaxing and rehydration sections were passed through xylol (#9713.3, Roth, Karlsruhe, Germany) and decreasing concentrations of EtOH (#200-678-6; Fisher Scientific, Waltham, Massachusetts, USA) until the solution evenly flowed across the slide ...
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bioRxiv - Bioengineering 2020Quote: ... During the course of the experiment, a syringe driver (Harvard, MKCB2159V) was used to deoxygenate the blood with 0.03 % w/v sodium hydrosulfite (ACROS Organics 7775-14-6) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA). PCR cycling ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Genetics 2022Quote: ... The styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64, 514/670 nm absorption/emission Invitrogen™, Waltham, Massachusetts) was used at a final concentration of 16.5 μM in ddH2O from a 16.5 mM stock solution in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... HPCs in suspension were collected and spun down at 300 xG for 6 minutes and resuspended into microglia basal media (MBM, 2mL/well) containing: DMEM/F12 no phenol (Gibco # 11-039-021), 2% Insulin Transferin Selenite (Gibco # 41400045) ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Physiology 2019Quote: ... larvae in 35 mm Petri dishes with 5 ml of E2 medium to the vortex flows caused by the spinning movements of small magnetic stir bars (6 × 3mm, Fischerbrand, #11888882, Fisher scientific, Leicestershire, UK.) inside the dishes ...
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bioRxiv - Physiology 2019Quote: ... containing thirty pre-exposed or control 10 dpf larvae and three magnetic stir bars (25 × 6 mm, Fisherbrand, #10226853, Fisher scientific, Leicestershire, UK) distributed equidistantly along the bottom of the cage were placed on top of the magnetic stirrer plate (Variomag ...
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bioRxiv - Developmental Biology 2019Quote: ... After 4 days the embryoid bodies were transferred to 24 well or 6 well plates coated with collagen (Thermo Scientific®; A10483-01) containing Melanocyte conversion media (45% DMEM – High glucose ...
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bioRxiv - Cell Biology 2019Quote: ... fibres were plated into 6-well plates (~30 fibres per well) in DMSO/high glucose/GlutaMAX supplement/pyruvate (Thermo Fisher Scientific, Cat# 31966021), containing 10% horse serum and 1% Penicillin/streptomycin solution ...
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bioRxiv - Physiology 2019Quote: ... animals were fasted for 6 hours and a single dose of 200□ mg STZ per kg body weight in phosphate buffered saline (PBS, Thermo Fisher Scientific, USA) was injected intraperitoneally ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... This primer mix was further diluted with 0.9 µL of UltraPure water then combined with 6 µL of PowerUp™ SYBR™ Green (ThermoFisher, Waltham, USA) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: Total RNA of THP-1 monocytes derived macrophages cultured in different circumstance for 7 days was digested from 6-well plates using trypsin and then TRIzol™ Reagent (Cat. #15596026, ThermoFisher Scientific, USA) was added ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amount of mRNA samples were subjected to qPCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) in a 384 plate ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Microbiology 2020Quote: ... Then RNA from corresponding sample was reverse transcribed into cDNA and the transcriptional levels of different genes were determined by qRT-PCR using SybrGreen Premix Low rox (MDbio, China) and the QuantStudioTM 6 Flex (Thermo Fisher Scientific, USA). The PCR condition was set as following ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR was performed on five replicates from each of the cultivars using an Applied Biosystems™ QuantStudio™ 6 Flex (Life Technologies). The reaction was performed in a 10 µl reaction consisting of two µL 1:20 diluted synthesised cDNA ...
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bioRxiv - Genetics 2020Quote: ... and 9ul Lipofectamine® reagent per well of a 6 well plate was each mixed with 150ul Optim-MEM I reduced serum media (Thermo Fisher Scientific, #31985062). After 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and the soluble fractions were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 6% Tris-Glycine gels (Life Technologies, Carlsbad, CA)64 ...
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bioRxiv - Physiology 2019Quote: ... Then cells were seeded on Collagenase I coated petri dishes (Collagen I, Coated Plate 6 Well, Gibco Life technologies A11428-01, Waltham MA), incubated at 32 to 35°C45 with 5% CO2 with high calcium DMEM medium overnight46 ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... or Nunc 6-well plates (2.5×10 cells/well) were or 2,5 μ jetPRIME® Polyplus transfection reagent or Lipofectamine 2000® (Thermo Fisher Scientific); according to the manufacturers’ instructions ...
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bioRxiv - Physiology 2020Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA) using the mouse primers shown in Table 1 ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured and expanded in 6-well plates coated with Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (A1413202, Gibco Life Technologies) diluted 1:100 in DMEM (31966021 ...
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bioRxiv - Pathology 2021Quote: ... Detailed immunohistochemistry staining and quantification procedures for each marker have been published (6, 16–26) or are in preparation for estrogen receptor alpha (antibody SP1; Thermo Scientific, Waltham, MA) and an antibody (PPG5/10 ...
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with (pRMB EDEN15) and BASU plasmids (6:1 ratio) using Lipofectamine 2000 (1:1 ratio, Life Technologies, California, USA). 40 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 500,00 cells were seeded into a 6-well format and were reverse transfected with 20 nM of gene-specific siRNA oligos using RNAiMax (Thermo Fisher Scientific #13778075) and then transfected again after 24 hours using forward transfection with 20 nM of siRNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... EndoC-βH1 cells were plated at a density of 1.37□105 cells/cm2 in 24 or 6 well plates and transfected after 24h using Lipofectamine 2000 (cat. 11668-019, Invitrogen, Pittsburgh, PA, USA) with different combinations of CRTC1 siRNA and/or miR-184 inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: ... Germany) and 2 drops of glacial acetic acid (#3738.1; Roth, Karlsruhe, Germany) in 70% EtOH (#200-678-6, Fisher Scientific, Waltham, Massachusetts, USA) for 30 s and subsequently rinsed in aqua bidest ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Developmental Biology 2022Quote: ... The 293T cells were seeded in a 6-well plate with coverslips and grown to 70% confluence for transfection with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000001) as recommended in the kit literature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
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bioRxiv - Microbiology 2022Quote: ... monolayers of HEK293T cells with 70% confluency were seeded in 6 well plate and was transfected with Lipofectamine 2000 (Thermo Fisher Scientific, USA) using various concentrations of siRNA (30 ...