Labshake search
Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Purified PCR products and fluorescent protein expression plasmids were digested with the corresponding restricted enzymes (ThermoFisher, see Table S1 for details) and then cleaned up with a PCR purification kit ...
-
bioRxiv - Plant Biology 2022Quote: ... of the AtHB23 gene was amplified with specific oligonucleotides (Supplementary Table S1) and cloned into the pMDC83 (Ka+) Gateway® vector system (Invitrogen), the Myc-pBA vector bearing the amino-terminal six-myc tag ...
-
bioRxiv - Microbiology 2023Quote: ... This gene was amplified using primer pair C01/C02 (see supplementary Table S1 for all primer sequences) and subcloned into pJET1.2/blunt (Thermo Fisher Scientific). Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB ...
-
bioRxiv - Bioengineering 2022Quote: ... 3D printed adapters and wires are washed with 70% ethanol and then further sterilized in the S1 cell culture hood (Safe 2020, Thermo Scientific) via UV-treatment for 1 hr ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Syber green-based qPCR was performed using primer sets (Table S1) with 50 ng of cDNA from each sample in the QuanStudio-6 qPCR system (Applied Biosystems) with thermocycle condition ...
-
bioRxiv - Plant Biology 2024Quote: ... the MpROPGAP and MpREN coding sequences were amplified using PCR by KOD Plus Neo DNA polymerase (TOYOBO) with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). To generate proMpE2F:XVE>>MpROPCA ...
-
bioRxiv - Plant Biology 2024Quote: ... were amplified from wild-type Tak-1 genomic DNA using PCR with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). These entry vectors were used in the Gateway LR reaction (Life Technologies ...
-
bioRxiv - Zoology 2024Quote: ... The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology) and specific primers (Table S1) upon ABI 3500 Genetic Analyzer (Applied Biosystems). The genome sequence of the M segment-coding region has been deposited in GenBank (accession numbers ...
-
bioRxiv - Microbiology 2024Quote: ... the adaptor-complementary primer P1 (Supplementary Table S1) was used for reverse transcription of the terminal of each strand using SuperScript™ III Reverse Transcriptase (18080093, Invitrogen) at 50°C as described by the product instruction ...
-
bioRxiv - Genetics 2024Quote: ... and reference and alternate alleles (Additional File S1) were submitted to the AgriSeq primer design team for the Ion Torrent system (ThermoFisher Scientific). Targets were passed through the AgriSeq quality control process (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... FLAG-YB-1 and EGFP-YB-1 (S1 File) in human cells were synthesized and subcloned in the vector pcDNA3.1(+) at GeneArt (Thermo Fisher Scientific). FLAG-M2-2 gene was inserted between the NcoI and AflII restriction sites ...
-
bioRxiv - Molecular Biology 2023Quote: ... operating at 300 kV and a magnification at specimen level corresponding to a pixel size of 2.27 Å (Table S1) controlled with the software EPU 1.8 (Thermo Fisher Scientific). Motion correction of the original movies was done using MotionCor2 (61) ...
-
bioRxiv - Microbiology 2023Quote: ... or 1 ml of unpurified passages of S1/HER2nhd or LS1D were subjected to RNA extraction via Trizol-LS (Life Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: The coding region of the Omp33 gene with an upstream region of 450 bp was amplified by PCR using gene-specific primers (Table S1) with AccuPrime™ Pfx SuperMix (Invitrogen) and cloned into the pVRL1Z plasmid using restriction enzymes EcoRI and NotI ...
-
bioRxiv - Biophysics 2023Quote: ... obtained from either healthy donors or donors with type2 diabetes (Table S1) and were cultured overnight in CMRL medium 1066 (Fisher Scientific) prior to initiating experiments.
-
bioRxiv - Microbiology 2023Quote: ... Expression of csuAB pili gene expression by RT-qPCR was performed with cDNA using the qPCR primer pairs listed in Table S1 and following the protocol for Fast SYBR Green Master Mix (Applied Biosystems) using a StepOnePlus real-time PCR instrument (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl in water) in presence or absence of 40 U/mL of the S1 nuclease (18001-016, ThermoFisher Scientific), and incubated at R.T ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl in water) in presence or absence of 40 U/mL of the S1 nuclease (18001-016, ThermoFisher Scientific), and incubated at R.T ...
-
bioRxiv - Biochemistry 2023Quote: ... FabWT F1 and SER variants (S1-S7) were checked by size exclusion chromatography using a Superdex 200 Increase 10/300 column (Fisher Scientific) and monitoring elution at 215 nm ...
-
bioRxiv - Plant Biology 2023Quote: ... Genotyping was performed with primers listed in Supplemental Table S1 using the Phire Tissue Direct PCR Master Mix (Thermo Fisher, #F170L). The BR-related mutants bzr1-1D ...
-
bioRxiv - Plant Biology 2023Quote: ... A total of 395 bp was amplified using specific primers (zma-394b-F/zma-mir394b-R; Table S1) and cloned into pCR8-GW vector (Thermo Fisher). The amplified region includes the annotated 122 bp hairpin (GRMZM5G871952 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We extracted DNA from all samples (Table S1) using the PureLink Microbiome DNA Purification Kit (Invitrogen, Thermo Fisher; Waltham MA, USA) according to the manufacturer’s instructions and following mechanical crushing using sterile L-shaped spreaders and a 2 min bead beating step ...
-
bioRxiv - Plant Biology 2023Quote: ... While qPCR was done to quantify the level of DTD2 overexpression for DTD2 overexpression line with appropriate primers (Table S1) and Power SYBR™ Green PCR Master Mix (ThermoFisher). Reactions were carried out in a Bio-Rad CFX384 thermocycler ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We extracted DNA from all samples (Table S1) using the PureLink Microbiome DNA Purification Kit (Invitrogen, Thermo Fisher; Waltham MA, USA) according to the manufacturer’s instructions and following mechanical crushing using sterile L-shaped spreaders and a 2 min bead beating step ...
-
bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (Table S1) were incubated for 1 hour and cell nuclei were counterstained using DAPI (Thermo Fisher Scientific; Cat# 62248).
-
bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... grown in 12-well plates in DMEM supplemented with 10% FCS (Gibco-BRL) and glutamine ...
-
bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... Opti-MEM and fetal calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
-
bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific), 50µM β-mercaptoethanol (Sigma) ...
-
bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in FACS buffer (5% FCS in PBS, Life Technologies). After adding Fcγ receptor block (1:100 dilution ...
-
bioRxiv - Genomics 2019Quote: A375 cells were grown in DMEM + 10% FCS + Penicillin/streptomycin (Gibco, Carlsbad, MA) and were treated with 10,000 J/m2 UVB 310 nm (7 mins @ 25 J/m2/s ...
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific). Imaging was performed in a heating chamber preheated to 37 °C and placed on an inverted microscope (IX83 from Olympus ...
-
bioRxiv - Systems Biology 2020Quote: ... PHHs were cultivated in Williams medium E (Biochrom) supplemented with 10% FCS (Gibco), 100 nM dexamethasone ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: ... All media were supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
-
bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FCS and 1% Non-essential amino acids (Thermo Fisher #11140050)) ...
-
bioRxiv - Cell Biology 2021Quote: ... washed in sterile Schneider’s medium containing 20% fetal calf serum (Schneider’s/FCS; Gibco), and eventually homogenized with micro-pestles in 1.5 centrifuge tubes containing 21 embryos per 100 μl dispersion medium (Prokop et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Interpath services) and 2mM L-glutamine (Invitrogen) at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain leukocytes were washed and blocked with mouse Fc Block (ThermoFisher, clone 93) prior to staining with primary antibody-conjugated flourophores ...
-
bioRxiv - Immunology 2020Quote: ... 2.5% FCS and 100 IU/ml penicillin and 100 mg/ml streptomycin (Gibco). CD4+ T cells were isolated from PBMC using CD4+ T cell isolation kit (Miltenyi Biotec ...
-
bioRxiv - Microbiology 2021Quote: ... Colorimetric changes were measured using an ELISA microplate reader (MULTISKAN FC, Thermo Scientific) at 450 nm ...
-
bioRxiv - Molecular Biology 2020Quote: ... Opti-MEM and fetal Calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
-
bioRxiv - Immunology 2020Quote: ... incubated in RPMI containing 10% FCS and 0.2 mg/ml Collagenase IV (GIBCO) for 30 min and then passed through a 19G needle to obtain a homogenous cell suspension ...
-
bioRxiv - Immunology 2021Quote: ... Lonza) with 10% fetal calf serum (FCS) with low LPS (HyClone, Thermo Scientific) and 10% conditioned L929 media as a source of CSF-1 ...
-
bioRxiv - Genetics 2019Quote: ... + 10% FCS (Biological Industries, Cromwell, USA) + 1% penicillin/streptomycin (Gibco-Life Technologies, USA) at 37°C with 5% CO2 ...
-
bioRxiv - Genetics 2019Quote: ... + 10% FCS (Biological Industries, Cromwell, USA) + 1% penicillin/streptomycin (Gibco-Life Technologies, USA) at 37°C with 5% CO2 ...
-
bioRxiv - Pathology 2019Quote: ... Plates were read at 405nm with a Multiskan FC® spectrophotometer (Thermo scientific).
-
Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... supplemented with 20% FCS and 1X insulin-transferin-sodium selenite (GIBCO, 41400-045). HEC-265 cells were cultured in EMEM (SIGMA ...