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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Free Biotin was removed using Zeba™ Spin Desalting Columns (Thermo Scientific™ 89882) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... EZ-Link NHS-PEG4-biotin or DyLight 650 NHS ester (both from ThermoFisher Scientific) was also added to the solution ...
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bioRxiv - Cancer Biology 2024Quote: ... All channels were blocked by injecting 100 μg/mL amine-PEG-biotin (Thermo Fisher). Biotin was also used in the buffer to ensure no non-specific binding of the ligase proteins to the sensorchip through the biotinylated-tags ...
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bioRxiv - Immunology 2024Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-Biotin (Thermo Scientific, 21217). Media was aspirated from wells and cells rinsed with PBS ...
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bioRxiv - Genetics 2022Quote: ... 20 μg of H3K9me2 antibody or 20 μg of H3K9me3 antibody (Hayashi-Takanaka et al. 2011) were preloaded to 40 μl Dynabeads M-280 Sheep anti-Mouse IgG (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... we used sheep Alba antiserum (kind gift of Malcolm White, University of St. Andrews, UK) in combination with donkey anti-sheep IgG Alexa488 (Thermo Fisher). Blots were scanned on a Typhoon FLA 9500 scanner (GE Lifesciences).
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bioRxiv - Genetics 2019Quote: ... 500mM NaCl using 5μg of mouse anti-DMC1 2H12/4 (Novus NB100-2617) pre-bound to 50μl of Dynabeads™ M-280 sheep anti-mouse IgG (Life Technologies).
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bioRxiv - Genetics 2019Quote: ... the sonicate was pre-cleared for 2h at 4°C with 65μl of Dynabeads™ M-280 sheep anti-rabbit IgG (Life Technologies) and a 1% input chromatin sample was set aside ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction was performed from isolate cultures grown overnight on Sheep Blood Agar by using the MagMax DNA Multi-Sample extraction kit (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... the reserved 1 mL of ham solution was subjected to decimal dilutions and plated on Columbia agar with 5% sheep blood (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with a polyclonal sheep anti-mouse THP (LifeSpan Bio, LS-C126349) and imaged using Super Signal Femto West Maximum Sensitivity Substrate (ThermoFisher Scientific). Blots were stripped using Restore Western Blot Stripping Buffer (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated overnight at 4°C with 1 mg/ml BSA and 4% sheep serum in PBST and one or more of the following: Alexa-fluor 488 Phalloidin 1:300 (Molecular Probes), anti-beta tubulin antibody 1:100 (E7 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were subsequently processed with donkey anti-sheep secondary antibody conjugated to Alexa Fluor 488 at 3 µl/ml and (Life Technologies), donkey-anti rabbit secondary antibody conjugated to Alexa Fluor 555 at 5 µl/ml (Life Technologies) ...
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bioRxiv - Pathology 2020Quote: ... the aAs isolates were grown on tryptone soya agar (TSA, Oxoid, UK) or blood agar (BA; TSA + 5% sheep blood Thermo Fisher) while the Vibrionaceae isolates were on sea water agar (SWA ...
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bioRxiv - Microbiology 2019Quote: ... single colonies were selected and grown separately on tryptic soy agar (TSA) plates with 5% sheep blood (blood agar) (ThermoFisher Scientific) under nonselective conditions ...
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bioRxiv - Cell Biology 2019Quote: ... Endothelial cells were then purified by a 10-minute incubation in suspension with magnetic sheep anti-rat Dynabeads (Invitrogen, Carlsbad, CA) coated with rat anti-mouse PECAM-1 (rat ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation in secondary antibodies (AlexaFluor488-donkey-anti-mouse, AlexaFluor594-donkey-anti-rabbit, AlexaFluor647-donkey-anti-sheep, ThermoFisher, 1:500) in PBS-TSP ...
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bioRxiv - Bioengineering 2021Quote: ... pyogenes was cultured on Trypticase™ Soy Agar (TSA II™) with Sheep Blood (BD BBL™, Fisher Scientific, Cat# B21239X). A sterile disposable inoculating loop (Globe Scientific ...
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bioRxiv - Immunology 2022Quote: ... A total of 250 µg of sonicated chromatin fragments were incubated with 10 µg of NAC1 antibody for crosslinking with magnetic beads (no. 11201D, Dynabeads® M280 sheep anti-mouse IgG, Dynal Biotech, Invitrogen). The cross-linked samples were reversed at 65°C for overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488 conjugated rabbit anti-sheep antibody (Jackson Immu-noResearch 313-545-045) for colocalization experiments or Alexa Fluor 488 conjugated anti-mouse antibody (Invitrogen 21042) for GFAP immunostaining in 5% BSA for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in sheep anti-TH (1:2000; Pelfreeze P60101-0) and rabbit anti-GFP (1:2000; Invitrogen A11122), or chicken anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were washed using TBS and incubated in secondary antibodies (Alexa Fluor-conjugated anti-rabbit/sheep 1:1000) and DAPI (Thermo Fisher Scientific Cat# D3571 ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep antibodies were incubated for 1h and after thorough washing and blocking with 5% normal goat serum (Life Technologies) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Colony forming units (CFUs) of frozen stocks were calculated based on serial dilution spotting on TSA + 5% sheep’s blood plates (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... or normal IgG (4 µg) was incubated with 50 µl Dynabeads M-280 sheep anti-mouse IgG magnetic beads (Thermo Fisher Scientific Cat# 11201D ...
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bioRxiv - Developmental Biology 2023Quote: ... Each PLAC-seq library was prepared using DpnII as the restriction enzyme and Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen #11203D) mixed with 5 µg of H3K4me3 (04-745 ...
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bioRxiv - Genomics 2023Quote: ... anti-FMRP and purchased anti-IgG (anti-rabbit IgG M-280 and anti-mouse IgG M-280 Dynabeads raised in sheep, Invitrogen, USA) were blocked to reduce background the night before the immunoprecipitation ...
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bioRxiv - Neuroscience 2023Quote: ... Mn) followed by a secondary antibody (1:200) coupled to a green (donkey anti-sheep AF488 Cat#A11015, Thermo Fisher Scientific) fluorophore for 3 hrs at RT ...
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bioRxiv - Microbiology 2023Quote: ... Columbia colistin and nalidixic acid (CNA) agar with 5% sheep blood (5%SB-CNA) and chocolate agar medium agar plates were purchased from Fisher Scientific.
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bioRxiv - Microbiology 2023Quote: ... pylori 26695 strains were grown on Trypticase Soy Agar II plates with 5% sheep blood and in Sulfite-Free Brucella Broth containing cholesterol (Gibco-BRL) (SFBB-chol ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 594-conjugated goat anti-mouse IgG (Cat# A-11032) and Alexa Fluor 647-conjugated donkey anti-sheep IgG (Cat# A-21448) secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 488-conjugated donkey antibody (Invitrogen A21206), (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31573). Washed three times for 30 minutes each in TNTT at 4 ℃ to remove secondary antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... We first incubated whole pre-fixed tissue in 1ml blocking solution (ice-cold PBS solution with 10% (v/v) Heat Inactivated Sheep Serum (HISS) (ThermoFisher, 16070096) and 0.5% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with specific primers (Table S1) using the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Systems Biology 2019Quote: GC-FID (Supplementary Figure S1) analysis was accomplished on the TRACE™ 1310 gas chromatograph (Thermo Fisher Scientific™, Austin, TX). The detector temperature was set at 305 °C where the ignition threshold was 0.5 Pa ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of biotinylated S1 protein (Genscripts) was used to saturate the binding sites on 1 µl of FluoroSphere neutravidin beads (Thermo Fisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ng of cDNA for each sample was preamplified using a 0.2X pool of 74 primers (TaqMan; Thermo Fisher; Table S1) for 18 cycles using PreAmp Master Mix (Fluidigm ...
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bioRxiv - Bioengineering 2021Quote: ... 9 mL blood samples (from the same pool) were poured into 9 mL S-Monovette opened syringes using S1 Pipet Filler (Thermo Scientific) which enabled fatigue-free pipetting ...
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bioRxiv - Microbiology 2019Quote: ... Remaining primers and viral ss/DNA were removed in a 50 µl reaction using 10 units S1 nuclease (#EN0321, Thermo Scientific) for 30 min at 25°C ...
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bioRxiv - Synthetic Biology 2022Quote: On Day 1: 500 µl of 10 µg/ml S1-hFc or RBD-mFc was coated in a 5 ml immunotube (NUNC, 444202) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with indicated cDNAs and primers (primer sequences are listed in Supplemental Table S1) in a QuantStudio Real-Time PCR system (Applied Biosystems). Expression levels were normalized by 18S rRNA.
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bioRxiv - Neuroscience 2019Quote: ... After thorough buffer rinses the tissues were then incubated with the appropriate secondary antibodies (Table S1) diluted 1:500 or with streptavidin conjugated to Alexa Fluor 488 (1:500, ThermoFisher, S32354) Alexa Fluor 555 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... and gene specific primer sets for the core clock genes (Integrated DNA Technologies; see Table S1) on the StepOne Real-Time PCR system (Applied Biosystems). Housekeeping genes were determined by running samples from patients (Human geNorm kit ...
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bioRxiv - Microbiology 2021Quote: Constructs based on pEGFP-C1 or 362 pCS mCherry DEST as listed in Table S1 were transiently transfected into HeLa cells for 16-20 hours using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific). Cells were then fixed in PBS with 4% paraformaldehyde for 20 min at room temperature and coverslips were mounted using ProLong diamond anti-fade mountant (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The genes of interest and pfhsp70-1LID- were PCR-amplified using specific primer pairs (SI Appendix, Table S1) and Platinum Taq DNA polymerase (ThermoFisher Scientific) (2 U/reaction) ...
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bioRxiv - Microbiology 2020Quote: ... The rclR-lacZ and rclX-lacZ plasmids were constructed by PCR amplification of the upstream promoter DNA (117 bp) of rclR and rclX using primers in Table S1 and ligation into pCR™-Blunt (Invitrogen) for sequence-verification using M13F and M13R primers ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the isolated DNA were examined by 1% agarose gels (Figure S1) and a Qubit Fluorometer v2.0 (Thermo Scientific, USA) respectively ...