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Citations for Thermo Fisher :
4151 - 4200 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen). All final clones were verified using full DNA sequence analysis.
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bioRxiv - Biochemistry 2022Quote: ... Each well was transfected with 360 ng of plasmid using Lipofectamine 3000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... pBv2-U6-6X and pBv1-U6-BB plasmids using Neon Transfection System (Invitrogen) under condition of 1050 V ...
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bioRxiv - Biochemistry 2022Quote: ... and the plasmid was introduced in BL21-star (DE3) cells (Thermo Fisher Scientific). GroEL expression was induced by adding 0.4 mM IPTG to the culture medium when the OD600 reached 0.65 ...
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bioRxiv - Biochemistry 2022Quote: ... DNA plasmids were introduced into cells using Lipofectamine 3000 (Life Technologies, Carlsbad, CA), at a concentration of 1 μg/well (3 μL of L-3000 and 2 μL P3000 ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5α and extracted by using PureLink™ HiPure Plasmid Miniprep Kit (Invitrogen). Antibodies were expressed using the ExpiCHO™ expression system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids expressing fusion protein were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using polyethyleneimine (Polysciences) ...
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bioRxiv - Microbiology 2022Quote: ... GFP plasmid is EGFP cloned into pcDNA DEST40 vector (Thermo Fisher Scientific 12274015). RIG-I plasmid DNA was generated by (Dittmann et al. ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Cell Biology 2022Quote: ... MEF cells were transiently transfected with plasmids above using Lipofectamine 3000 (Invitrogen, USA) following the manufacturer’s protocol and grown overnight on a round glass-bottom dish (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and c-Flag pcDNA3/ERα/ERβ plasmids using Lipofectamine 3000 (Thermo Fisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with 600ng total plasmid DNA using Lipofetamine 3000 reagent (Invitrogen). For experiment shown in Fig 1 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were transfected with plasmid DNA indicated in Results using Lipofectamine 2000 (Invitrogen). On the day of imaging ...
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bioRxiv - Biophysics 2022Quote: ... The linearized plasmid was in vitro transcribed using MEGAscriptTM T7 Transcription Kit (Invitrogen). The CV-B3-RLuc RNA was then electroporated into H1-Hela+CDHR3 cells using the Gene Pulser XcellTM Electroporation System ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transiently transfected using the ExpiFectamine 293 Transfection Kit and protocol (GIBCO).
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bioRxiv - Biochemistry 2022Quote: The resulting pApoA1-ApoM plasmid was transfected into adherent CHO-S cells (InvitroGen) using PEI (Polyethylenimine ...
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bioRxiv - Immunology 2022Quote: The generated plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using the FreeStyle MAX reagent ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then purified using the Quick Plasmid Purification Kit (Thermo Fisher Scientific, USA). Antisense probes were synthesized by in vitro transcription (MEGAScript Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmids were transfected using Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific) according to manufacture’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Transfection of plasmid (10 µg) or 10-20 nM synthetic siRNA (Life Technologies) was performed using Lipofectamine® 2000 transfection reagent (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant plasmids were linearized with PacI before transfection with Lipofectamine 2000 (ThermoFisher) into the adenovirus packaging cell lines HEK293 cells ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells were transfected with 750 ng of plasmids using Lipofectamine 2000 (Invitrogen) in 24-well-plates according to the manufacturer’s recommendations and washed 5 h later.
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bioRxiv - Microbiology 2021Quote: ... and subsequently cloned into the pACYC177 plasmid using the Subcloning Service (Invitrogen, Germany). All plasmids were sequenced to check for the presence of the desired point mutations and to ensure there were no unwanted secondary mutations.
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant BACs were purified using PureLink™ HiPure Plasmid Midiprep Kit (ThermoFisher Scientific) and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Next day 1 μl of reporter plasmid was transfected using Lipofectamine2000 (Life Technologies). After 48 h the cells were lysed and luminescence was measured with Dual-Glo Luciferase assay system (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection into HEK293T cells was performed using Lipofectamine 2000 (Thermo Fisher Scientific) or polyethylenimine (PEI) ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific) and Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 and donor plasmids were co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) into HEK293E cells which were seeded in a 6-well plate one day before transfection ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding heavy and light chains were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... along with 3μg pAX2 and 2μg VSVG packaging plasmids using Lipofectamine 3000 (Invitrogen). 48 hours later ...
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bioRxiv - Microbiology 2021Quote: ... PCR products and plasmids were digested with Eco52I and KpnI (Thermo Fisher Scientific), purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... After 24 hours cells were transfected with indicated plasmids using Lipofectamine 3000 (Invitrogen) in Opti-MEM™ medium ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were transiently transfected into CHO cells using ExpiFectamine CHO Transfection Kit (ThermoFisher), and protein was purified one the sixth day post-transfection ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were transiently transfected with EMTB-3XGFP plasmid using the Neon (ThermoFisher Scientific) electroporation system two days before imaging ...
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bioRxiv - Molecular Biology 2022Quote: ... Sense and antisense oligos were annealed and cloned into pENTR/U6 plasmid (Invitrogen). Using LR recombination (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... and plasmids encoding various S genes by using Lipofectamine 3000 (Invitrogen, L3000-015). The supernatants were harvested at 24 ± 2 h post transfection and centrifuged at 1500rpm for 5 min to remove cell debris and then stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids containing Cas9 and single-guide RNAs were transfected (Lipofectamine 2000; 11668019; Invitrogen) into the LAMP1Δ-RUSH cell line according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... purified (reference) plasmids were quantified (individually) by fluorometry (Qubit™, Thermo Fisher Scientific), and copy number estimated ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids used for transfection were generated using the Gateway cloning system (Life technologies): transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC ...
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bioRxiv - Biochemistry 2022Quote: ... 50μg of the LentiCRISPRv2 hygro plasmid was digested with BsmBI (ThermoFisher cat # FD0454) and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher cat # EF0654 ...
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bioRxiv - Genomics 2022Quote: ... the plasmid was first digested and dephosphorylated with FastDigest BsmBI and FastAP (Thermofisher) at 37°C for 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids were introduced into the two cell types using Lipofectamine (Gibco-BRL) according to the manufacturer instruction ...
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bioRxiv - Biophysics 2019Quote: ... were transformed with 100 ng of pET24a His8–MBP plasmid (Thermo Fisher Scientific) encoding the desired HEV constructs and incubated overnight at 37 °C on LB Agar plates containing kanamycin (50 μg/mL) ...
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bioRxiv - Genetics 2020Quote: ... The plasmids were transformed to Stellar™ Competent Cells (Invitrogen, cat. no. 636766) using a standard heat shock protocol (30 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... and transfected with plasmids encoding indicated Gag-YFP derivatives using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was introduced into Escherichia coli BL21 Star (DE3) (Life Technologies, USA). The resulting expression strain was pre-grown for 16 hours in liquid culture at 37 °C in LB supplemented with 50 μg ml−1 ampicillin ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-ANK or pEGFP-SET plasmids using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). HDFs were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: ... 500μL of DMEM containing 4μg plasmid mixed with 10μL Lipofectmine 2000 (Thermo Fisher) was added to each well of the 6-well culture plate when cells grew to 60-70% confluency ...
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bioRxiv - Genomics 2019Quote: ... Ampicillin resistant colonies were collected and extracted for plasmids using Maxiprep kit (Invitrogen). Ten sampling clones from the agar plate were analyzed by PCR and Sanger sequencing to verify successful cassette insertion and barcode diversity (Primer details in Table S1).