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Citations for Thermo Fisher :
4101 - 4150 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid concentration was quantified using a Nanodrop 2000 (Thermo Scientific, Waltham, MA). One microliter of each dilution was used as a template for LAMP.
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bioRxiv - Molecular Biology 2020Quote: ... 1ug digested plasmid was added to mMessage mMachine SP6 reaction (Ambion, Austin, TX) followed by DNA digestion and RNA clean-up using RNA Clean and Concentrator kit (Zymo ...
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bioRxiv - Molecular Biology 2020Quote: Cells were transfected with plasmid DNA (Supplementary Table 3) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 µg of 53BP1-expression plasmids were transfected using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer guidelines ...
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bioRxiv - Genomics 2020Quote: ... The resulting plasmids were transfected into HeLa cells using Lipofectamine 2000 (Life Technologies). These cells were engineered to contain a single loxP-flanked cassette within their genome (Khandelia et al. ...
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bioRxiv - Biochemistry 2019Quote: pET151/D-TOPO plasmids containing the sequences of interest were acquired from Invitrogen; fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene ...
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bioRxiv - Genetics 2019Quote: ... Entry clone plasmids were propagated in One Shot Top10 chemically competent E.coli (Invitrogen) with kanamycin 50 micrograms/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... mCherry-NLS-DEF6 plasmid was establish by inserting annealed NLS oligos (ThermoFisher Scientific) between mCherry and DEF6 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with indicated plasmids using Lipofectamine 2000 (#10696153, Thermo Fisher Scientific).
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... Transfection assays for plasmids were performed using Lipofectamine 2000 (Invitrogen; Carlsbad, CA, US) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Ligated plasmid was used to transform DH5α E.coli cells per manufacturer’s instructions (Invitrogen). Colonies were isolated from LB plates containing 50 µg/ml ampicillin and at least 10 colonies per construct were grown in 100 µl of LB containing ampicillin at 37 °C ON ...
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bioRxiv - Genetics 2019Quote: ... This plasmid was transfected into INS832/13 cells using Lipofectamine 2000 (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Genetics 2019Quote: ... A total of 2 μg plasmid DNA was transfected using Lipofectamine 2000 (Invitrogen). For metabolic labeling ...
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bioRxiv - Microbiology 2019Quote: ... and introduced into the plasmid with restriction enzyme-based techniques (Thermo Scientific, Finland). The ligated plasmid was transformed into E ...
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bioRxiv - Genomics 2020Quote: ... f1 G8PPD plasmid (1.5 μg) were transfected into cells using Lipofectamine 3000 (Invitrogen). At 24 h post G8PPD transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... pCS2nCas9n plasmid and mMessage mMachine SP6 in vitro transcription kits (Thermo Fisher Scientific) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... All plasmids and siRNAs were transfected into different cells using lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Plasmids producing the Sup35NMG protein were based on either pYES2 (Thermo Fisher Scientific) or pCM190 (46 ...
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bioRxiv - Developmental Biology 2019Quote: The amplified products were cloned into the pENTR plasmid (Life Technologies, cat # K240020), digested with Eco RI and Bam HI ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfection was achieved by electroporation with the NEON transfection system (Invitrogen, MPK5000). 36 hours post transfection cells were FACS sorted for Cas9-BFP and 1000-2000 cells seeded for clonal expansion on a 15cm plate ...
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bioRxiv - Genetics 2020Quote: ... and quantified plasmid DNA using Qubit dsDNA HS Assay Kit (Thermo Fisher #Q32854). We digested 10 µg of RCP83 using 10 µL SfiI enzyme (NEB ...
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bioRxiv - Cancer Biology 2020Quote: USP18 cDNA plasmids were transfected with Lipofectamine LTX and Plus (Invitrogen #15338-100) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: The pPvvCY-p230p-gfpLuc plasmid was constructed using MultiSite Gateway cloning system (Invitrogen). attB-flanked 5’and 3’homology arms were obtained by amplifying 800bp regions upstream and downstream of PVVCY_0300700 ...
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Tuning Cell-free Composition Controls the Time-delay, Dynamics, and Productivity of TX-TL ExpressionbioRxiv - Synthetic Biology 2021Quote: ... after which any remaining plasmid DNA was removed via digestion with TurboDNase (Invitrogen). RNA integrity was verified via agarose gel electrophoresis ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: 3 × 104 cells were transfected BRE-Luciferase reporter plasmid using Lipofectamine 2000 (Invitrogen). The cell lysates collected 48 hr after transfection ...
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bioRxiv - Bioengineering 2021Quote: ... pcDNA3.1-Sox2 plasmids were constructed and transfected using Lipofectamine 3000 (Thermo Fisher Scientific).The expression levels of nestin (forward primer ...
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bioRxiv - Neuroscience 2020Quote: ... and 24h after plasmid transfection using Lipofectamine® LTX and Plus reagent (Invitrogen). siRNA sequences used are ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transformed and grown in subcloning efficiency DH5α competent cells (Invitrogen # 18265017) before extraction by Miniprep (QIAGEN #27104 ...
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bioRxiv - Cancer Biology 2021Quote: ... Coli competent cells and amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). Diagnostic PCR with the MIGR1 primers set ...
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bioRxiv - Cancer Biology 2020Quote: ... Reporter plasmids were transfected at 200ng per 12-well with lipofectamine 3000 (Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Resulting plasmids were introduced into E.coli DH5α competent cells (ThermoFisher, Breda, the Netherlands) by heat shock transformation ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective construct plasmid using TurboFect (Thermo Fisher Scientific, Waltham, MA, USA). After 48 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... Expression constructs were assembled from pENTR plasmids using Gateway cloning (Thermo Fisher Scientific). All plasmids are described in Supplementary Table 3.
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bioRxiv - Genetics 2020Quote: ... LentiCas9-puro-sGArid1a or LentiCas9-Blast plasmid by Lipofectamine 3000 (Life Technologies # L3000015). Virus containing medium was collected at 60h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... 0.25 μg of plasmid DNA and 0.375 μl Lipofectamine™ 2000 (#743517, Invitrogen) were mixed for one well of a 24-well plate ...
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bioRxiv - Microbiology 2020Quote: STm SL1344-GFP (with pFPV25.1 plasmid) was maintained under Ampicillin selection (11593027, Gibco). STm SL1344 wildtype strain was maintained in the presence of streptomycin (11860038 ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into mammalian cells by using Lipofectamine 3000 (Invitrogen, cat# L3000150). After 24 h transfection ...
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bioRxiv - Synthetic Biology 2021Quote: ... Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific). Transformations were performed in accordance with the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Parental 690.cl2 cells were transfected with that plasmid using Lipofectamine (Life Technologies) following manufacturer’s instructions and GFP positive cells were single sorted into 96 well plates after 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA and siRNA transfections were performed using lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The lentiviral plasmid was transfected along with the ViraPower lentiviral expression system (ThermoFisher), following the recommended protocol from the vendor ...
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bioRxiv - Cell Biology 2021Quote: HeLa or HEK293T cells were transfected with plasmids using Lipofectamine 3000 (ThermoFisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmids was performed by using lipofectamine 2000™ transfection reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: PX459-sgRNA plasmid (2μg) was transfected using 10 μL of Lipofectamine 2000 (Invitrogen). After 4 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then transfected with the following plasmid concentrations using lipofectamine 2000 (Invitrogen): p1A1-FL (244 ng/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfections of plasmids were performed with Lipofectamine® LTX and Plus Reagent (Invitrogen), siRNAs were transfected using Lipofectamin RNAiMAX ...
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bioRxiv - Cell Biology 2022Quote: The pCMV-Gaussia-Dura Luc and pTK-Red Firefly Luc plasmids from ThermoFisher were used to construct the dual-luciferase reporter plasmid (pGL-RF) ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids were transfected into HEK293 cells in triplicate using Lipofectamine 2000 (Thermofisher Scientific) in the presence of various amounts of DHT ...